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Article

Salvia miltiorrhiza induces depolarization of pacemaker potentials in murine small intestinal interstitial cells of Cajal via extracellular Ca2+ and Na+ influx

  • Authors:
    • Donghun Han
    • Jeong Nam Kim
    • Min Ji Kwon
    • Taewon Han
    • Yun Tai Kim
    • Byung Joo Kim
  • View Affiliations / Copyright

    Affiliations: Division of Longevity and Biofunctional Medicine, Pusan National University School of Korean Medicine, Yangsan, Gyeongsangnam‑do 50612, Republic of Korea, Korea Food Research Institute, Wanju‑gun, Jeollabuk‑do 55365, Republic of Korea
  • Article Number: 348
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    Published online on: March 11, 2021
       https://doi.org/10.3892/mmr.2021.11987
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Abstract

Interstitial cells of Cajal (ICCs) are pacemaker cells that control smooth muscle contraction in the gastrointestinal (GI) tract. The present study investigated the effects of Salvia miltiorrhiza (SM) on the pacemaker potentials of ICCs from the mouse small intestine in vitro and on GI motility in vivo. The whole‑cell patch‑clamp configuration was used to record pacemaker potential in ICCs in vitro, and GI motility was investigated in vivo by recording intestinal transit rate (ITR). Using the whole‑cell patch‑clamp configuration, SM depolarized the pacemaker potentials of ICCs in a dose‑dependent manner. Fulvestrant blocked SM‑induced effects but 1,3‑dihydro‑3,3‑bis(4‑hydroxyphenyl)-7-methyl‑2H‑indol‑2‑one did not. Additionally, 4‑[2‑phenyl-5,7‑bis(trifluoromethyl) pyrazolo[1,5‑a]pyrimidin‑3‑yl] phenol blocked SM‑induced effects. Intracellular guanosine 5'‑O‑(2‑thiodiphosphate), and pretreatment with extracellular Ca2+‑ and Na+‑free solutions also blocked SM‑induced effects. Furthermore, ITR values were increased by SM in vivo and SM elevated the levels of motilin (MTL). The SM‑induced increase in ITR was associated with increased protein expression levels of c‑kit and the transmembrane protein 16A (TMEM16A) channel. In addition, SM induced pacemaker potential depolarization through estrogen receptor β in a G protein‑dependent manner via extracellular Ca2+ and Na+ regulation in the murine small intestine in vitro. Moreover, SM increased the ITR in vivo through the MTL hormone via c‑kit and TMEM16A‑dependent pathways. Taken together, these results suggested that SM may have the ability to control GI motility and could be used as a GI motility regulator.
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Copy and paste a formatted citation
Spandidos Publications style
Han D, Kim J, Kwon M, Han T, Kim Y and Kim B: <em>Salvia miltiorrhiza</em> induces depolarization of pacemaker potentials in murine small intestinal interstitial cells of Cajal via extracellular Ca<sup>2+</sup> and Na<sup>+</sup> influx. Mol Med Rep 23: 348, 2021.
APA
Han, D., Kim, J., Kwon, M., Han, T., Kim, Y., & Kim, B. (2021). <em>Salvia miltiorrhiza</em> induces depolarization of pacemaker potentials in murine small intestinal interstitial cells of Cajal via extracellular Ca<sup>2+</sup> and Na<sup>+</sup> influx. Molecular Medicine Reports, 23, 348. https://doi.org/10.3892/mmr.2021.11987
MLA
Han, D., Kim, J., Kwon, M., Han, T., Kim, Y., Kim, B."<em>Salvia miltiorrhiza</em> induces depolarization of pacemaker potentials in murine small intestinal interstitial cells of Cajal via extracellular Ca<sup>2+</sup> and Na<sup>+</sup> influx". Molecular Medicine Reports 23.5 (2021): 348.
Chicago
Han, D., Kim, J., Kwon, M., Han, T., Kim, Y., Kim, B."<em>Salvia miltiorrhiza</em> induces depolarization of pacemaker potentials in murine small intestinal interstitial cells of Cajal via extracellular Ca<sup>2+</sup> and Na<sup>+</sup> influx". Molecular Medicine Reports 23, no. 5 (2021): 348. https://doi.org/10.3892/mmr.2021.11987
Copy and paste a formatted citation
x
Spandidos Publications style
Han D, Kim J, Kwon M, Han T, Kim Y and Kim B: <em>Salvia miltiorrhiza</em> induces depolarization of pacemaker potentials in murine small intestinal interstitial cells of Cajal via extracellular Ca<sup>2+</sup> and Na<sup>+</sup> influx. Mol Med Rep 23: 348, 2021.
APA
Han, D., Kim, J., Kwon, M., Han, T., Kim, Y., & Kim, B. (2021). <em>Salvia miltiorrhiza</em> induces depolarization of pacemaker potentials in murine small intestinal interstitial cells of Cajal via extracellular Ca<sup>2+</sup> and Na<sup>+</sup> influx. Molecular Medicine Reports, 23, 348. https://doi.org/10.3892/mmr.2021.11987
MLA
Han, D., Kim, J., Kwon, M., Han, T., Kim, Y., Kim, B."<em>Salvia miltiorrhiza</em> induces depolarization of pacemaker potentials in murine small intestinal interstitial cells of Cajal via extracellular Ca<sup>2+</sup> and Na<sup>+</sup> influx". Molecular Medicine Reports 23.5 (2021): 348.
Chicago
Han, D., Kim, J., Kwon, M., Han, T., Kim, Y., Kim, B."<em>Salvia miltiorrhiza</em> induces depolarization of pacemaker potentials in murine small intestinal interstitial cells of Cajal via extracellular Ca<sup>2+</sup> and Na<sup>+</sup> influx". Molecular Medicine Reports 23, no. 5 (2021): 348. https://doi.org/10.3892/mmr.2021.11987
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