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Berberine ameliorates the LPS-induced imbalance of osteogenic and adipogenic differentiation in rat bone marrow-derived mesenchymal stem cells

  • Authors:
    • Rong Zhou
    • Fubo Chen
    • Haixia Liu
    • Xueqin Zhu
    • Xueyun Wen
    • Fang Yu
    • Guangwei Shang
    • Shengcai Qi
    • Yuanzhi Xu
  • View Affiliations / Copyright

    Affiliations: Department of Stomatology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai 200072, P.R. China
    Copyright: © Zhou et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 350
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    Published online on: March 11, 2021
       https://doi.org/10.3892/mmr.2021.11989
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Abstract

Lipopolysaccharide (LPS) from oral pathogenic bacteria is an important factor leading to alveolar bone absorption and the implant failure. The present study aimed to evaluate the modulation of berberine hydrochloride (BBR) on the LPS-mediated osteogenesis and adipogenesis imbalance in rat bone marrow-derived mesenchymal stem cells (BMSCs). Cell viability, osteoblastic and adipogenic differentiation levels were measured using the Cell Counting Kit-8 assay, alkaline phosphatase (ALP) staining and content assay, and oil red O staining, respectively. Reverse transcription-quantitative PCR and immunoblotting were used to detect the related gene and protein expression levels. In undifferentiated cells, BBR increased the mRNA expression levels of the osteoblastic genes (Alp, RUNX family transcription factor 2, osteocalcin and secreted phosphoprotein 1) but not the adipogenic genes (fatty acid binding protein 4, Adipsin and peroxisome proliferator-activated receptorγ). LPS-induced osteoblastic gene downregulation, adipogenic gene enhancement and NF-κB activation were reversed by BBR treatment. In osteoblastic differentiated cells, decreased ALP production by LPS treatment was recovered with BBR co-incubation. In adipogenic differentiated cells, LPS-mediated lipid accumulation was decreased by BBR administration. The mRNA expression levels of the pro-inflammatory factors (MCP-1, TNF-α, IL-6 and IL-1β) were increased by LPS under both adipogenic and osteoblastic conditions, which were effectively ameliorated by BBR. The actions of BBR were attenuated by compound C, suggesting that the role of BBR may be partly due to AMP-activated protein kinase activation. The results demonstrated notable pro-osteogenic and anti-adipogenic actions of BBR in a LPS-stimulated inflammatory environment. This indicated a potential role of BBR for bacterial infected-related peri-implantitis medication.
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Copy and paste a formatted citation
Spandidos Publications style
Zhou R, Chen F, Liu H, Zhu X, Wen X, Yu F, Shang G, Qi S and Xu Y: Berberine ameliorates the LPS-induced imbalance of osteogenic and adipogenic differentiation in rat bone marrow-derived mesenchymal stem cells. Mol Med Rep 23: 350, 2021.
APA
Zhou, R., Chen, F., Liu, H., Zhu, X., Wen, X., Yu, F. ... Xu, Y. (2021). Berberine ameliorates the LPS-induced imbalance of osteogenic and adipogenic differentiation in rat bone marrow-derived mesenchymal stem cells. Molecular Medicine Reports, 23, 350. https://doi.org/10.3892/mmr.2021.11989
MLA
Zhou, R., Chen, F., Liu, H., Zhu, X., Wen, X., Yu, F., Shang, G., Qi, S., Xu, Y."Berberine ameliorates the LPS-induced imbalance of osteogenic and adipogenic differentiation in rat bone marrow-derived mesenchymal stem cells". Molecular Medicine Reports 23.5 (2021): 350.
Chicago
Zhou, R., Chen, F., Liu, H., Zhu, X., Wen, X., Yu, F., Shang, G., Qi, S., Xu, Y."Berberine ameliorates the LPS-induced imbalance of osteogenic and adipogenic differentiation in rat bone marrow-derived mesenchymal stem cells". Molecular Medicine Reports 23, no. 5 (2021): 350. https://doi.org/10.3892/mmr.2021.11989
Copy and paste a formatted citation
x
Spandidos Publications style
Zhou R, Chen F, Liu H, Zhu X, Wen X, Yu F, Shang G, Qi S and Xu Y: Berberine ameliorates the LPS-induced imbalance of osteogenic and adipogenic differentiation in rat bone marrow-derived mesenchymal stem cells. Mol Med Rep 23: 350, 2021.
APA
Zhou, R., Chen, F., Liu, H., Zhu, X., Wen, X., Yu, F. ... Xu, Y. (2021). Berberine ameliorates the LPS-induced imbalance of osteogenic and adipogenic differentiation in rat bone marrow-derived mesenchymal stem cells. Molecular Medicine Reports, 23, 350. https://doi.org/10.3892/mmr.2021.11989
MLA
Zhou, R., Chen, F., Liu, H., Zhu, X., Wen, X., Yu, F., Shang, G., Qi, S., Xu, Y."Berberine ameliorates the LPS-induced imbalance of osteogenic and adipogenic differentiation in rat bone marrow-derived mesenchymal stem cells". Molecular Medicine Reports 23.5 (2021): 350.
Chicago
Zhou, R., Chen, F., Liu, H., Zhu, X., Wen, X., Yu, F., Shang, G., Qi, S., Xu, Y."Berberine ameliorates the LPS-induced imbalance of osteogenic and adipogenic differentiation in rat bone marrow-derived mesenchymal stem cells". Molecular Medicine Reports 23, no. 5 (2021): 350. https://doi.org/10.3892/mmr.2021.11989
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