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Hydrogen sulfide improves ox‑LDL‑induced expression levels of Lp‑PLA2 in THP‑1 monocytes via the p38MAPK pathway

  • Authors:
    • Heng-Jing Hu
    • Jie Qiu
    • Chi Zhang
    • Zhi-Han Tang
    • Shun-Lin Qu
    • Zhi-Sheng Jiang
  • View Affiliations / Copyright

    Affiliations: Department of Cardiology Laboratory, First Affiliated Hospital of University of South China, Hengyang, Hunan 421001, P.R. China, Department of Cardiology Laboratory, Huazhong University of Science and Technology Tongji Medical College First Clinical College, Wuhan, Hubei 430000, P.R. China, Institute of Cardiovascular Disease and Key Lab for Arteriosclerology of Hunan Province, University of South China, Hengyang, Hunan 421001, P.R. China
    Copyright: © Hu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 358
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    Published online on: March 12, 2021
       https://doi.org/10.3892/mmr.2021.11997
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Abstract

Hydrogen sulfide (H2S) exerts an anti‑atherosclerotic effect and decreases foam cell formation. Lipoprotein‑associated phospholipase A2 (Lp‑PLA2) is a key factor involved in foam cell formation. However, the association between H2S and Lp‑PLA2 expression levels with respect to foam cell formation has not yet been elucidated. The present study investigated whether H2S can affect foam cell formation and potential signalling pathways via regulation of the expression and activity of Lp‑PLA2. Using human monocytic THP‑1 cells as a model system, it was observed that oxidized low‑density lipoprotein (ox‑LDL) not only upregulates the expression level and activity of Lp‑PLA2, it also downregulates the expression level and activity of Cystathionine γ lyase. Exogenous supplementation of H2S decreased the expression and activity of Lp‑PLA2 induced by ox‑LDL. Moreover, ox‑LDL induced the expression level and activity of Lp‑PLA2 via activation of the p38MAPK signalling pathway. H2S blocked the expression levels and activity of Lp‑PLA2 induced by ox‑LDL via inhibition of the p38MAPK signalling pathway. Furthermore, H2S inhibited Lp‑PLA2 activity by blocking the p38MAPK signaling pathway and significantly decreased lipid accumulation in ox‑LDL‑induced macrophages, as detected by Oil Red O staining. The results of the present study indicated that H2S inhibited ox‑LDL‑induced Lp‑PLA2 expression levels and activity by blocking the p38MAPK signalling pathway, thereby improving foam cell formation. These findings may provide novel insights into the role of H2S intervention in the progression of atherosclerosis.
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Copy and paste a formatted citation
Spandidos Publications style
Hu H, Qiu J, Zhang C, Tang Z, Qu S and Jiang Z: Hydrogen sulfide improves ox‑LDL‑induced expression levels of Lp‑PLA<sub>2</sub> in THP‑1 monocytes via the p38MAPK pathway. Mol Med Rep 23: 358, 2021.
APA
Hu, H., Qiu, J., Zhang, C., Tang, Z., Qu, S., & Jiang, Z. (2021). Hydrogen sulfide improves ox‑LDL‑induced expression levels of Lp‑PLA<sub>2</sub> in THP‑1 monocytes via the p38MAPK pathway. Molecular Medicine Reports, 23, 358. https://doi.org/10.3892/mmr.2021.11997
MLA
Hu, H., Qiu, J., Zhang, C., Tang, Z., Qu, S., Jiang, Z."Hydrogen sulfide improves ox‑LDL‑induced expression levels of Lp‑PLA<sub>2</sub> in THP‑1 monocytes via the p38MAPK pathway". Molecular Medicine Reports 23.5 (2021): 358.
Chicago
Hu, H., Qiu, J., Zhang, C., Tang, Z., Qu, S., Jiang, Z."Hydrogen sulfide improves ox‑LDL‑induced expression levels of Lp‑PLA<sub>2</sub> in THP‑1 monocytes via the p38MAPK pathway". Molecular Medicine Reports 23, no. 5 (2021): 358. https://doi.org/10.3892/mmr.2021.11997
Copy and paste a formatted citation
x
Spandidos Publications style
Hu H, Qiu J, Zhang C, Tang Z, Qu S and Jiang Z: Hydrogen sulfide improves ox‑LDL‑induced expression levels of Lp‑PLA<sub>2</sub> in THP‑1 monocytes via the p38MAPK pathway. Mol Med Rep 23: 358, 2021.
APA
Hu, H., Qiu, J., Zhang, C., Tang, Z., Qu, S., & Jiang, Z. (2021). Hydrogen sulfide improves ox‑LDL‑induced expression levels of Lp‑PLA<sub>2</sub> in THP‑1 monocytes via the p38MAPK pathway. Molecular Medicine Reports, 23, 358. https://doi.org/10.3892/mmr.2021.11997
MLA
Hu, H., Qiu, J., Zhang, C., Tang, Z., Qu, S., Jiang, Z."Hydrogen sulfide improves ox‑LDL‑induced expression levels of Lp‑PLA<sub>2</sub> in THP‑1 monocytes via the p38MAPK pathway". Molecular Medicine Reports 23.5 (2021): 358.
Chicago
Hu, H., Qiu, J., Zhang, C., Tang, Z., Qu, S., Jiang, Z."Hydrogen sulfide improves ox‑LDL‑induced expression levels of Lp‑PLA<sub>2</sub> in THP‑1 monocytes via the p38MAPK pathway". Molecular Medicine Reports 23, no. 5 (2021): 358. https://doi.org/10.3892/mmr.2021.11997
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