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LINC01116 promotes the proliferation and invasion of glioma by regulating the microRNA‑744‑5p‑MDM2‑p53 axis

  • Authors:
    • Li Jiang
    • Chao Cheng
    • Wei Ji
    • Hao Wang
    • Quan Du
    • Xiaoqiao Dong
    • Junfei Shao
    • Wenhua Yu
  • View Affiliations / Copyright

    Affiliations: Department of Neurosurgery, Hangzhou First People's Hospital of Nanjing Medical University, Hangzhou, Zhejiang 310006, P.R. China, Department of Neurosurgery, Wuxi People's Hospital of Nanjing Medical University, Wuxi, Jiangsu 214023, P.R. China
    Copyright: © Jiang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 366
    |
    Published online on: March 16, 2021
       https://doi.org/10.3892/mmr.2021.12005
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Abstract

Long non‑coding RNAs (lncRNAs) have been implicated in the development and progression of tumors. However, the roles and underlying mechanisms of long intergenic non‑protein coding RNA 1116 (LINC01116), a member of the lncRNA family, in glioma progression are largely unclear. The expression of LINC01116 and microRNA (miR)‑744‑5p in glioma tissues and cells was detected by reverse transcription‑quantitative PCR. The influences of LINC01116 or miR‑744‑5p on cell proliferation and invasion were evaluated by Cell Counting Kit‑8, colony formation and Transwell assays, and western blotting was used to detect the expression of p53 pathway proteins. A dual‑luciferase reporter system was used to locate common binding sites between miR‑744‑5p and LINC01116 or the 3' untranslated region of E3 ubiquitin‑protein ligase Mdm2 (MDM2). RNA immunoprecipitation was used to determine the interactions between RNAs and proteins. Moreover, a xenograft mouse model was constructed to investigate the effects of LINC01116 in vivo, followed by a TdT‑mediated dUTP nick end labeling assay to determine the degree of apoptosis in nude mouse tumors. LINC01116 was found to be highly expressed in glioma tissues, which was associated with a malignant phenotype. LINC01116 promoted the proliferation and invasiveness of glioma cells, and inhibited the p53 pathway by preserving the expression of MDM2 mRNA via miR‑744‑5p sponging. Furthermore, a low degree of miR‑744‑5p expression was observed in glioma tissues, which was negatively associated with the expression of LINC01116. Overexpression of miR‑744‑5p inhibited the proliferation and invasiveness of glioma cells, which was rescued by LINC01116. Finally, LINC01116 knockdown inhibited tumor growth in nude mice. In conclusion, LINC01116 is aberrantly expressed and promotes the progression of glioma by regulating the miR‑744‑5p‑MDM2‑p53 pathway. In future, targeting LINC01116 may therefore be a potential therapeutic approach for patients with glioma.
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Copy and paste a formatted citation
Spandidos Publications style
Jiang L, Cheng C, Ji W, Wang H, Du Q, Dong X, Shao J and Yu W: LINC01116 promotes the proliferation and invasion of glioma by regulating the microRNA‑744‑5p‑MDM2‑p53 axis. Mol Med Rep 23: 366, 2021.
APA
Jiang, L., Cheng, C., Ji, W., Wang, H., Du, Q., Dong, X. ... Yu, W. (2021). LINC01116 promotes the proliferation and invasion of glioma by regulating the microRNA‑744‑5p‑MDM2‑p53 axis. Molecular Medicine Reports, 23, 366. https://doi.org/10.3892/mmr.2021.12005
MLA
Jiang, L., Cheng, C., Ji, W., Wang, H., Du, Q., Dong, X., Shao, J., Yu, W."LINC01116 promotes the proliferation and invasion of glioma by regulating the microRNA‑744‑5p‑MDM2‑p53 axis". Molecular Medicine Reports 23.5 (2021): 366.
Chicago
Jiang, L., Cheng, C., Ji, W., Wang, H., Du, Q., Dong, X., Shao, J., Yu, W."LINC01116 promotes the proliferation and invasion of glioma by regulating the microRNA‑744‑5p‑MDM2‑p53 axis". Molecular Medicine Reports 23, no. 5 (2021): 366. https://doi.org/10.3892/mmr.2021.12005
Copy and paste a formatted citation
x
Spandidos Publications style
Jiang L, Cheng C, Ji W, Wang H, Du Q, Dong X, Shao J and Yu W: LINC01116 promotes the proliferation and invasion of glioma by regulating the microRNA‑744‑5p‑MDM2‑p53 axis. Mol Med Rep 23: 366, 2021.
APA
Jiang, L., Cheng, C., Ji, W., Wang, H., Du, Q., Dong, X. ... Yu, W. (2021). LINC01116 promotes the proliferation and invasion of glioma by regulating the microRNA‑744‑5p‑MDM2‑p53 axis. Molecular Medicine Reports, 23, 366. https://doi.org/10.3892/mmr.2021.12005
MLA
Jiang, L., Cheng, C., Ji, W., Wang, H., Du, Q., Dong, X., Shao, J., Yu, W."LINC01116 promotes the proliferation and invasion of glioma by regulating the microRNA‑744‑5p‑MDM2‑p53 axis". Molecular Medicine Reports 23.5 (2021): 366.
Chicago
Jiang, L., Cheng, C., Ji, W., Wang, H., Du, Q., Dong, X., Shao, J., Yu, W."LINC01116 promotes the proliferation and invasion of glioma by regulating the microRNA‑744‑5p‑MDM2‑p53 axis". Molecular Medicine Reports 23, no. 5 (2021): 366. https://doi.org/10.3892/mmr.2021.12005
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