Sevoflurane inhibits cell proliferation and migration of glioma by targeting the miR‑27b/VEGF axis

Zhan,Xi; Lei,Changcheng; Yang,Linzhu

doi:10.3892/mmr.2021.12047

Sevoflurane inhibits cell proliferation and migration of glioma by targeting the miR‑27b/VEGF axis

Authors:
- Xi Zhan
- Changcheng Lei
- Linzhu Yang
View Affiliations

Affiliations: Department of Anesthesiology, The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650032, P.R. China
Published online on: March 29, 2021 https://doi.org/10.3892/mmr.2021.12047
Article Number: 408
Copyright: © Zhan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

Poor prognosis in patients with glioma is primarily due to rapid tumor growth and cell invasion and migration. In addition, microRNA (miR)‑27b is decreased in metastatic glioma. The present study investigated whether sevoflurane inhibited glioma cell progression by targeting miR‑27b. Cell proliferation was analyzed using a Cell Counting Kit‑8 assay and a wound healing assay was used to detect cell migration. Western blotting and reverse transcription‑quantitative PCR analysis were performed to determine the protein and mRNA expression levels. A dual luciferase assay was used to determine the relationship between vascular epithelial growth factor (VEGF) and miR‑27b. VEGF was identified to be a direct target of miR‑27b. Moreover, sevoflurane treatment increased the expression of miR‑27b and decreased the expression of VEGF in U251 and U87 cells. Compared with the control group, sevoflurane inhibited the proliferation and migration of U251 and U87 cells, as well as the expression of matrix metalloproteinase (MMP)‑2 and MMP‑9, which were subsequently abolished by pre‑treatment with an miR‑27b inhibitor. The present results indicated the potential use of sevoflurane by anesthesiologists for the surgical resection of glioma, which may improve patient outcomes in the clinical setting.

View Figures

View References

1	Mamelak AN and Jacoby DB: Targeted delivery of antitumoral therapy to Glioma and other malignancies with synthetic chlorotoxin (TM-601). Expert Opin Drug Deliv. 4:175–186. 2007. View Article : Google Scholar : PubMed/NCBI
2	Radner H, El-Shabrawi Y, Eibl RH, Brüstle O, Kenner L, Kleihues P and Wiestler OD: Tumor induction by ras and myc oncogenes in fetal and neonatal brain: Modulating effects of developmental stage and retroviral dose. Acta Neuropathol. 86:456–465. 1993. View Article : Google Scholar : PubMed/NCBI
3	Wong ET and Brem S: Taming glioblastoma: Targeting angiogenesis. J Clin Oncol. 25:4705–4706. 2007. View Article : Google Scholar : PubMed/NCBI
4	Jiang B, Chaichana K, Veeravagu A, Chang SD, Black KL and Patil CG: Biopsy versus resection for the management of low-grade gliomas. The Cochrane Database of Systematic Reviews. 4:CD0093192017.PubMed/NCBI
5	Stewart L and Burdett S; Glioma Meta-analysis Trialists Group (GMT), : Chemotherapy for high-grade glioma. Cochrane Database Syst Rev. CD0039132012.
6	Davis FG and McCarthy BJ: Current epidemiological trends and surveillance issues in brain tumors. Expert Rev Anticancer Ther. 1:395–401. 2001. View Article : Google Scholar : PubMed/NCBI
7	Giese A, Bjerkvig R, Berens ME and Westphal M: Cost of migration: Invasion of malignant gliomas and implications for treatment. J Clin Oncol. 21:1624–1636. 2003. View Article : Google Scholar : PubMed/NCBI
8	Snyder GL and Greenberg S: Effect of anaesthetic technique and other perioperative factors on cancer recurrence. Br J Anaesth. 105:106–115. 2010. View Article : Google Scholar : PubMed/NCBI
9	Kvolik S, Glavas-Obrovac L, Bares V and Karner I: Effects of inhalation anesthetics halothane, sevoflurane, and isoflurane on human cell lines. Life Sci. 77:2369–2383. 2005. View Article : Google Scholar : PubMed/NCBI
10	Kvolik S, Dobrosevic B, Marczi S, Prlic L and Glavas-Obrovac L: Different apoptosis ratios and gene expressions in two human cell lines after sevoflurane anaesthesia. Acta Anaesthesiol Scand. 53:1192–1199. 2009. View Article : Google Scholar : PubMed/NCBI
11	Liang H, Gu M, Yang C, Wang H, Wen X and Zhou Q: Sevoflurane inhibits invasion and migration of lung cancer cells by inactivating the p38 MAPK signaling pathway. J Anesth. 26:381–392. 2012. View Article : Google Scholar : PubMed/NCBI
12	Goto G, Hori Y, Ishikawa M, Tanaka S and Sakamoto A: Changes in the gene expression levels of microRNAs in the rat hippocampus by sevoflurane and propofol anesthesia. Mol Med Rep. 9:1715–1722. 2014. View Article : Google Scholar : PubMed/NCBI
13	Ambros V: microRNAs: Tiny regulators with great potential. Cell. 107:823–826. 2001. View Article : Google Scholar : PubMed/NCBI
14	Bartel DP: MicroRNAs: Genomics, biogenesis, mechanism, and function. Cell. 116:281–297. 2004. View Article : Google Scholar : PubMed/NCBI
15	Kloosterman WP and Plasterk RH: The diverse functions of microRNAs in animal development and disease. Dev Cell. 11:441–450. 2006. View Article : Google Scholar : PubMed/NCBI
16	Ciafre SA, Galardi S, Mangiola A, Ferracin M, Liu CG, Sabatino G, Negrini M, Maira G, Croce CM and Farace MG: Extensive modulation of a set of microRNAs in primary glioblastoma. Biochem Biophys Res Commun. 334:1351–1358. 2005. View Article : Google Scholar : PubMed/NCBI
17	Feng Q, Wu X, Li F, Ning B, Lu X, Zhang Y, Pan Y and Guan W: miR-27b inhibits gastric cancer metastasis by targeting NR2F2. Protein Cell. 8:114–122. 2017. View Article : Google Scholar : PubMed/NCBI
18	Sun Y, Xu T, Cao YW and Ding XQ: Antitumor effect of miR-27b-3p on lung cancer cells via targeting Fzd7. Eur Rev Med Pharmacol Sci. 21:4113–4123. 2017.PubMed/NCBI
19	Luo Y, Yu SY, Chen JJ, Qin J, Qiu YE, Zhong M and Chen M: MiR-27b directly targets Rab3D to inhibit the malignant phenotype in colorectal cancer. Oncotarget. 9:3830–3841. 2017. View Article : Google Scholar : PubMed/NCBI
20	Khan FH, Pandian V, Ramraj S, Aravindan S, Herman TS and Aravindan N: Reorganization of metastamiRs in the evolution of metastatic aggressive neuroblastoma cells. BMC Genomics. 16:5012015. View Article : Google Scholar : PubMed/NCBI
21	Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI
22	Yi W, Li D, Guo Y, Zhang Y, Huang B and Li X: Sevoflurane inhibits the migration and invasion of glioma cells by upregulating microRNA-637. Int J Mol Med. 38:1857–1863. 2016. View Article : Google Scholar : PubMed/NCBI
23	Lin J, Teo S, Lam DH, Jeyaseelan K and Wang S: MicroRNA-10b pleiotropically regulates invasion, angiogenicity and apoptosis of tumor cells resembling mesenchymal subtype of glioblastoma multiforme. Cell Death Dis. 3:e3982012. View Article : Google Scholar : PubMed/NCBI
24	Song L, Huang Q, Chen K, Liu L, Lin C, Dai T, Yu C, Wu Z and Li J: miR-218 inhibits the invasive ability of glioma cells by direct downregulation of IKK-β. Biochem Biophys Res Commun. 402:135–140. 2010. View Article : Google Scholar : PubMed/NCBI
25	Fan YC, Mei PJ, Chen C, Miao FA, Zhang H and Li ZL: MiR-29c inhibits glioma cell proliferation, migration, invasion and angiogenesis. J Neurooncol. 115:179–188. 2013. View Article : Google Scholar : PubMed/NCBI
26	Chen Y, Zhang B, Jin Y, Wu Q and Cao L: MiR-27b targets PI3K p110α to inhibit proliferation and migration in colorectal cancer stem cell. Am J Transl Res. 11:5988–5997. 2019.PubMed/NCBI
27	Sun W, Zhang L, Yan R, Yang Y and Meng X: LncRNA DLX6-AS1 promotes the proliferation, invasion, and migration of non-small cell lung cancer cells by targeting the miR-27b-3p/GSPT1 axis. Onco Targets Ther. 12:3945–3954. 2019. View Article : Google Scholar : PubMed/NCBI
28	Liu C, Liang S, Xiao S, Lin Q, Chen X, Wu Y and Fu J: MicroRNA-27b inhibits Spry2 expression and promotes cell invasion in glioma U251 cells. Oncol Lett. 9:1393–1397. 2015. View Article : Google Scholar : PubMed/NCBI
29	Chen L, Li H, Han L, Zhang K, Wang G, Wang Y, Liu Y, Zheng Y, Jiang T, Pu P, et al: Expression and function of miR-27b in human glioma. Oncol Rep. 26:1617–1621. 2011.PubMed/NCBI
30	Wen MM: Getting miRNA therapeutics into the target cells for neurodegenerative diseases: A mini-review. Front Mol Neurosci. 9:1292016. View Article : Google Scholar : PubMed/NCBI
31	Dhup S, Dadhich RK, Porporato PE and Sonveaux P: Multiple biological activities of lactic acid in cancer: Influences on tumor growth, angiogenesis and metastasis. Curr Pharm Des. 18:1319–1330. 2012. View Article : Google Scholar : PubMed/NCBI
32	Yancopoulos GD: Clinical application of therapies targeting VEGF. Cell. 143:13–16. 2010. View Article : Google Scholar : PubMed/NCBI
33	Stetler-Stevenson WG and Seo DW: TIMP-2: An endogenous inhibitor of angiogenesis. Trends M ol Med. 11:97–103. 2005. View Article : Google Scholar
34	Tapia A, Salamonsen LA, Manuelpillai U and Dimitriadis E: Leukemia inhibitory factor promotes human first trimester extravillous trophoblast adhesion to extracellular matrix and secretion of tissue inhibitor of metalloproteinases-1 and −2. Hum Reprod. 23:1724–1732. 2008. View Article : Google Scholar : PubMed/NCBI