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Changes in the phosphorylation of nucleotide metabolism‑associated proteins by leukemia inhibitory factor in mouse embryonic stem cells

  • Authors:
    • Hwa-Ryung Song
    • Han-Kyu Kim
    • Seung-Gook Kim
    • Hyung-Jin Lim
    • Hyun-Yi Kim
    • Myung-Kwan Han
  • View Affiliations / Copyright

    Affiliations: Department of Microbiology, Jeonbuk National University Medical School, Jeonju, Jeollabuk 54896, Republic of Korea, Division of Anatomy and Developmental Biology, Department of Oral Biology, Yonsei University College of Dentistry, Seoul 03722, Republic of Korea
    Copyright: © Song et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 431
    |
    Published online on: April 8, 2021
       https://doi.org/10.3892/mmr.2021.12070
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Abstract

Leukemia inhibitory factor (LIF) is a stem cell growth factor that maintains self‑renewal of mouse embryonic stem cells (mESCs). LIF is a cytokine in the interleukin‑6 family and signals via the common receptor subunit gp130 and ligand‑specific LIF receptor. LIF causes heterodimerization of the LIF receptor and gp130, activating the Janus kinase/STAT and MAPK pathways, resulting in changes in protein phosphorylation. The present study profiled LIF‑mediated protein phosphorylation changes in mESCs via proteomic analysis. mESCs treated in the presence or absence of LIF were analyzed via two‑dimensional differential in‑gel electrophoresis and protein and phosphoprotein staining. Protein identification was performed by matrix‑assisted laser desorption/ionization‑time of flight mass spectrophotometry. Increased phosphorylation of 16 proteins and decreased phosphorylation of 34 proteins in response to LIF treatment was detected. Gene Ontology terms enriched in these proteins included ‘organonitrogen compound metabolic process’, ‘regulation of mRNA splicing via spliceosome' and 'nucleotide metabolic process’. The present results revealed that LIF modulated phosphorylation levels of nucleotide metabolism‑associated proteins, thus providing insight into the mechanism underlying LIF action in mESCs.
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1 

Martin GR: Isolation of a pluripotent cell line from early mouse embryos cultured in medium conditioned by teratocarcinoma stem cells. Proc Natl Acad Sci USA. 78:7634–7638. 1981. View Article : Google Scholar : PubMed/NCBI

2 

Thomson JA, Itskovitz-Eldor J, Shapiro SS, Waknitz MA, Swiergiel JJ, Marshall VS and Jones JM: Embryonic stem cell lines derived from human blastocysts. Science. 282:1145–1147. 1998. View Article : Google Scholar : PubMed/NCBI

3 

Aladjem MI, Spike BT, Rodewald LW, Hope TJ, Klemm M, Jaenisch R and Wahl GM: ES cells do not activate p53-dependent stress responses and undergo p53-independent apoptosis in response to DNA damage. Curr Biol. 8:145–155. 1998. View Article : Google Scholar : PubMed/NCBI

4 

Takahashi K, Tanabe K, Ohnuki M, Narita M, Ichisaka T, Tomoda K and Yamanaka S: Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell. 131:861–872. 2007. View Article : Google Scholar : PubMed/NCBI

5 

Yu J, Vodyanik MA, Smuga-Otto K, Antosiewicz-Bourget J, Frane JL, Tian S, Nie J, Jonsdottir GA, Ruotti V, Stewart R, et al: Induced pluripotent stem cell lines derived from human somatic cells. Science. 318:1917–1920. 2007. View Article : Google Scholar : PubMed/NCBI

6 

Rajarajan K, Engels MC and Wu SM: Reprogramming of mouse, rat, pig, and human fibroblasts into iPS cells. Curr Protoc Mol Biol. Jan 1–2012.(Epub ahead of print). doi: 10.1002/0471142727.mb2315s97. View Article : Google Scholar : PubMed/NCBI

7 

Niwa H, Burdon T, Chambers I and Smith A: Self-renewal of pluripotent embryonic stem cells is mediated via activation of STAT3. Genes Dev. 12:2048–2060. 1998. View Article : Google Scholar : PubMed/NCBI

8 

Ernst M, Oates A and Dunn AR: Gp130-mediated signal transduction in embryonic stem cells involves activation of Jak and Ras/mitogen-activated protein kinase pathways. J Biol Chem. 271:30136–30143. 1996. View Article : Google Scholar : PubMed/NCBI

9 

Migone TS, Rodig S, Cacalano NA, Berg M, Schreiber RD and Leonard WJ: Functional cooperation of the interleukin-2 receptor beta chain and Jak1 in phosphatidylinositol 3-kinase recruitment and phosphorylation. Mol Cell Biol. 18:6416–6422. 1998. View Article : Google Scholar : PubMed/NCBI

10 

Bechard M and Dalton S: Subcellular localization of glycogen synthase kinase 3beta controls embryonic stem cell self-renewal. Mol Cell Biol. 29:2092–2104. 2009. View Article : Google Scholar : PubMed/NCBI

11 

Hermanns HM, Radtke S, Schaper F, Heinrich PC and Behrmann I: Non-redundant signal transduction of interleukin-6-type cytokines. The adapter protein Shc is specifically recruited to rhe oncostatin M receptor. J Biol Chem. 275:40742–40748. 2000. View Article : Google Scholar : PubMed/NCBI

12 

Huang W, Sherman BT and Lempicki RA: Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources. Nat Protoc. 4:44–57. 2009. View Article : Google Scholar : PubMed/NCBI

13 

Walter W, Sánchez-Cabo F and Ricote M: GOplot: An R package for visually combining expression data with functional analysis. Bioinformatics. 31:2912–2914. 2015. View Article : Google Scholar : PubMed/NCBI

14 

Wei Q, Khan IK, Ding Z, Yerneni S and Kihara D: NaviGO: Interactive tool for visualization and functional similarity and coherence analysis with gene ontology. BMC Bioinformatics. 18:1772017. View Article : Google Scholar : PubMed/NCBI

15 

Toumadje A, Kusumoto K, Parton A, Mericko P, Dowell L, Ma G, Chen L, Barnes DW and Sato JD: Pluripotent differentiation in vitro of murine ES-D3 embryonic stem cells. In Vitro Cell Dev Biol Anim. 39:449–453. 2003. View Article : Google Scholar : PubMed/NCBI

16 

Meng GL, Zur Nieden NI, Liu SY, Cormier JT, Kallos MS and Rancourt DE: Properties of murine embryonic stem cells maintained on human foreskin fibroblasts without LIF. Mol Reprod Dev. 75:614–622. 2008. View Article : Google Scholar : PubMed/NCBI

17 

Johansson H and Simonsson S: Core transcription factors, Oct4, Sox2 and Nanog, individually form complexes with nucleophosmin (Npm1) to control embryonic stem (ES) cell fate determination. Aging (Albany NY). 2:815–822. 2010. View Article : Google Scholar : PubMed/NCBI

18 

Wu Y, Chen K, Xing G, Li L, Ma B, Hu Z, Duan L and Liu X: Phospholipid remodeling is critical for stem cell pluripotency by facilitating mesenchymal-to-epithelial transition. Sci Adv. 5:eaax75252019. View Article : Google Scholar : PubMed/NCBI

19 

Xu Y, Zhao W, Olson SD, Prabhakara KS and Zhou X: Alternative splicing links histone modifications to stem cell fate decision. Genome Biol. 19:1332018. View Article : Google Scholar : PubMed/NCBI

20 

Kwon YW, Ahn HS, Park JY, Yang HM, Cho HJ and Kim HS: Imprinted gene Zinc finger protein 127 is a novel regulator of master pluripotency transcription factor, Oct4. BMB Rep. 51:242–248. 2018. View Article : Google Scholar : PubMed/NCBI

21 

Pyo JH, Jeon HJ, Park JS, Lee JS, Chung HY and Yoo MA: Drosophila PEBP1 inhibits intestinal stem cell aging via suppression of ERK pathway. Oncotarget. 9:17980–17993. 2018. View Article : Google Scholar : PubMed/NCBI

22 

Ratnadiwakara M, Archer SK, Dent CI, Mozos IR, Beilharz TH, Knaupp AS, Nefzger CM, Polo JM and Anko ML: SRSF3 promotes pluripotency through Nanog mRNA export and coordination of the pluripotency gene expression program. Elife. 7:e374192018. View Article : Google Scholar : PubMed/NCBI

23 

Li J, Sejas DP, Rani R, Koretsky T, Bagby GC and Pang Q: Nucleophosmin regulates cell cycle progression and stress response in hematopoietic stem/progenitor cells. J Biol Chem. 281:16536–16545. 2006. View Article : Google Scholar : PubMed/NCBI

24 

Alekseev OM, Widgren EE, Richardson RT and O'Rand MG: Association of NASP with HSP90 in mouse spermatogenic cells: Stimulation of ATPase activity and transport of linker histones into nuclei. J Biol Chem. 280:2904–2911. 2005. View Article : Google Scholar : PubMed/NCBI

25 

Alekseev OM, Bencic DC, Richardson RT, Widgren EE and O'Rand MG: Overexpression of the linker histone-binding protein tNASP affects progression through the cell cycle. J Biol Chem. 278:8846–8852. 2003. View Article : Google Scholar : PubMed/NCBI

26 

Lomelino CL, Andring JT, McKenna R and Kilberg MS: Asparagine synthetase: Function, structure, and role in disease. J Biol Chem. 292:19952–19958. 2017. View Article : Google Scholar : PubMed/NCBI

27 

Gantt JS, Chiang CS, Hatfield GW and Arfin SM: Chinese hamster ovary cells resistant to beta-aspartylhydroxamate contain increased levels of asparagine synthetase. J Biol Chem. 255:4808–4813. 1980. View Article : Google Scholar : PubMed/NCBI

28 

Ortega JA, Nesbit ME Jr, Donaldson MH, Hittle RE, Weiner J, Karon M and Hammond D: L-Asparaginase, vincristine, and prednisone for induction of first remission in acute lymphocytic leukemia. Cancer Res. 37:535–540. 1977.PubMed/NCBI

29 

Zhang X, Xiao S, Rameau RD, Devany E, Nadeem Z, Caglar E, Ng K, Kleiman FE and Saxena A: Nucleolin phosphorylation regulates PARN deadenylase activity during cellular stress response. RNA Biol. 15:251–260. 2018. View Article : Google Scholar : PubMed/NCBI

30 

Yang A, Shi G, Zhou C, Lu R, Li H, Sun L and Jin Y: Nucleolin maintains embryonic stem cell self-renewal by suppression of p53 protein-dependent pathway. J Biol Chem. 286:43370–43382. 2011. View Article : Google Scholar : PubMed/NCBI

31 

Leach FS, Nicolaides NC, Papadopoulos N, Liu B, Jen J, Parsons R, Peltomäki P, Sistonen P, Aaltonen LA, Nyström-Lahti M, et al: Mutations of a mutS homolog in hereditary nonpolyposis colorectal cancer. Cell. 75:1215–1225. 1993. View Article : Google Scholar : PubMed/NCBI

32 

Christmann M, Tomicic MT and Kaina B: Phosphorylation of mismatch repair proteins MSH2 and MSH6 affecting MutSalpha mismatch-binding activity. Nucleic Acids Res. 30:1959–1966. 2002. View Article : Google Scholar : PubMed/NCBI

33 

Kwon SC, Yi H, Eichelbaum K, Föhr S, Fischer B, You KT, Castello A, Krijgsveld J, Hentze MW and Kim VN: The RNA-binding protein repertoire of embryonic stem cells. Nat Struct Mol Biol. 20:1122–1130. 2013. View Article : Google Scholar : PubMed/NCBI

34 

Chen K, Dai X and Wu J: Alternative splicing: An important mechanism in stem cell biology. World J Stem Cells. 7:1–10. 2015. View Article : Google Scholar : PubMed/NCBI

35 

Das S, Jena S and Levasseur DN: Alternative splicing produces Nanog protein variants with different capacities for self-renewal and pluripotency in embryonic stem cells. J Biol Chem. 286:42690–42703. 2011. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Song H, Kim H, Kim S, Lim H, Kim H and Han M: Changes in the phosphorylation of nucleotide metabolism‑associated proteins by leukemia inhibitory factor in mouse embryonic stem cells. Mol Med Rep 23: 431, 2021.
APA
Song, H., Kim, H., Kim, S., Lim, H., Kim, H., & Han, M. (2021). Changes in the phosphorylation of nucleotide metabolism‑associated proteins by leukemia inhibitory factor in mouse embryonic stem cells. Molecular Medicine Reports, 23, 431. https://doi.org/10.3892/mmr.2021.12070
MLA
Song, H., Kim, H., Kim, S., Lim, H., Kim, H., Han, M."Changes in the phosphorylation of nucleotide metabolism‑associated proteins by leukemia inhibitory factor in mouse embryonic stem cells". Molecular Medicine Reports 23.6 (2021): 431.
Chicago
Song, H., Kim, H., Kim, S., Lim, H., Kim, H., Han, M."Changes in the phosphorylation of nucleotide metabolism‑associated proteins by leukemia inhibitory factor in mouse embryonic stem cells". Molecular Medicine Reports 23, no. 6 (2021): 431. https://doi.org/10.3892/mmr.2021.12070
Copy and paste a formatted citation
x
Spandidos Publications style
Song H, Kim H, Kim S, Lim H, Kim H and Han M: Changes in the phosphorylation of nucleotide metabolism‑associated proteins by leukemia inhibitory factor in mouse embryonic stem cells. Mol Med Rep 23: 431, 2021.
APA
Song, H., Kim, H., Kim, S., Lim, H., Kim, H., & Han, M. (2021). Changes in the phosphorylation of nucleotide metabolism‑associated proteins by leukemia inhibitory factor in mouse embryonic stem cells. Molecular Medicine Reports, 23, 431. https://doi.org/10.3892/mmr.2021.12070
MLA
Song, H., Kim, H., Kim, S., Lim, H., Kim, H., Han, M."Changes in the phosphorylation of nucleotide metabolism‑associated proteins by leukemia inhibitory factor in mouse embryonic stem cells". Molecular Medicine Reports 23.6 (2021): 431.
Chicago
Song, H., Kim, H., Kim, S., Lim, H., Kim, H., Han, M."Changes in the phosphorylation of nucleotide metabolism‑associated proteins by leukemia inhibitory factor in mouse embryonic stem cells". Molecular Medicine Reports 23, no. 6 (2021): 431. https://doi.org/10.3892/mmr.2021.12070
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