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MicroRNA‑524‑5p regulates the proliferation and invasion of HTR‑8/SVneo trophoblasts by targeting NUMB in the Notch signaling pathway

  • Authors:
    • Linmei Zheng
    • Jie Song
    • Rong Tang
    • Xiaoju Chen
    • Li Wang
    • Dongcai Wu
    • Hui Cen
    • Lei Shi
  • View Affiliations / Copyright

    Affiliations: Department of Obstetrics, Hainan General Hospital, Hainan Affiliated Hospital of Hainan Medical University, Haikou, Hainan 570311, P.R. China
    Copyright: © Zheng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 436
    |
    Published online on: April 8, 2021
       https://doi.org/10.3892/mmr.2021.12075
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Abstract

Preeclampsia is a pregnancy disorder that is primarily associated with maternal and neonatal or fetal morbidity and mortality. The discovery of dysregulated microRNAs (miRs) and their roles in preeclampsia has provided new insight into the mechanisms involved in pregnancy‑related disorders. In the present study, quantitative PCR demonstrated that the expression levels of miR‑524‑5p were lower in patients with preeclampsia than those in normal pregnant women. Cell Counting Kit‑8 and Transwell assays indicated that overexpression of miR‑524‑5p promoted the proliferation and invasion of HTR‑8/SVneo cells, whereas inhibition of miR‑524‑5p suppressed HTR‑8/SVneo cell proliferation and invasion. Furthermore, NUMB endocytic adaptor protein (NUMB), a negative regulator of the Notch signaling pathway and a target gene of miR‑524‑5p, limited the effects of miR‑524‑5p on HTR‑8/SVneo cell invasion and migration. The present study demonstrated that miR‑524‑5p regulated the proliferation and invasion of HTR‑8/SVneo cells at least partly by targeting NUMB to regulate the Notch signaling pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Zheng L, Song J, Tang R, Chen X, Wang L, Wu D, Cen H and Shi L: MicroRNA‑524‑5p regulates the proliferation and invasion of HTR‑8/SVneo trophoblasts by targeting NUMB in the Notch signaling pathway. Mol Med Rep 23: 436, 2021.
APA
Zheng, L., Song, J., Tang, R., Chen, X., Wang, L., Wu, D. ... Shi, L. (2021). MicroRNA‑524‑5p regulates the proliferation and invasion of HTR‑8/SVneo trophoblasts by targeting NUMB in the Notch signaling pathway. Molecular Medicine Reports, 23, 436. https://doi.org/10.3892/mmr.2021.12075
MLA
Zheng, L., Song, J., Tang, R., Chen, X., Wang, L., Wu, D., Cen, H., Shi, L."MicroRNA‑524‑5p regulates the proliferation and invasion of HTR‑8/SVneo trophoblasts by targeting NUMB in the Notch signaling pathway". Molecular Medicine Reports 23.6 (2021): 436.
Chicago
Zheng, L., Song, J., Tang, R., Chen, X., Wang, L., Wu, D., Cen, H., Shi, L."MicroRNA‑524‑5p regulates the proliferation and invasion of HTR‑8/SVneo trophoblasts by targeting NUMB in the Notch signaling pathway". Molecular Medicine Reports 23, no. 6 (2021): 436. https://doi.org/10.3892/mmr.2021.12075
Copy and paste a formatted citation
x
Spandidos Publications style
Zheng L, Song J, Tang R, Chen X, Wang L, Wu D, Cen H and Shi L: MicroRNA‑524‑5p regulates the proliferation and invasion of HTR‑8/SVneo trophoblasts by targeting NUMB in the Notch signaling pathway. Mol Med Rep 23: 436, 2021.
APA
Zheng, L., Song, J., Tang, R., Chen, X., Wang, L., Wu, D. ... Shi, L. (2021). MicroRNA‑524‑5p regulates the proliferation and invasion of HTR‑8/SVneo trophoblasts by targeting NUMB in the Notch signaling pathway. Molecular Medicine Reports, 23, 436. https://doi.org/10.3892/mmr.2021.12075
MLA
Zheng, L., Song, J., Tang, R., Chen, X., Wang, L., Wu, D., Cen, H., Shi, L."MicroRNA‑524‑5p regulates the proliferation and invasion of HTR‑8/SVneo trophoblasts by targeting NUMB in the Notch signaling pathway". Molecular Medicine Reports 23.6 (2021): 436.
Chicago
Zheng, L., Song, J., Tang, R., Chen, X., Wang, L., Wu, D., Cen, H., Shi, L."MicroRNA‑524‑5p regulates the proliferation and invasion of HTR‑8/SVneo trophoblasts by targeting NUMB in the Notch signaling pathway". Molecular Medicine Reports 23, no. 6 (2021): 436. https://doi.org/10.3892/mmr.2021.12075
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