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miR‑654‑5p inhibits autophagy by targeting ATG7 via mTOR signaling in intervertebral disc degeneration

  • Authors:
    • Shanzheng Wang
    • Yudong Guo
    • Xuejun Zhang
    • Chen Wang
  • View Affiliations / Copyright

    Affiliations: Department of Orthopaedics, Zhongda Hospital, Medical School of Southeast University, Nanjing, Jiangsu 210009, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 444
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    Published online on: April 12, 2021
       https://doi.org/10.3892/mmr.2021.12083
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Abstract

Intervertebral disc degeneration (IDD) is a common chronic disease characterized by the loss of extracellular matrix (ECM) in the nucleus pulposus (NP). Accumulating evidence has revealed that abnormal expression of microRNAs (miRs) is closely associated with IDD development. The present study aimed to investigate the precise role and possible mechanism underlying the effects of miR‑654‑5p in the pathogenesis of IDD. NP cells were isolated from patients with IDD. Monodansylcadaverine staining was conducted to reveal cell autophagy, while western blotting was performed to detect the expression of ECM‑related proteins in NP cells. Luciferase reporter and RNA immunoprecipitation assays were conducted to identify the binding between RNAs. The results demonstrated that miR‑654‑5p was significantly upregulated in degenerated NP tissues from patients with IDD and high miR‑654‑5p expression was positively associated with disc degeneration grade. Functional assays suggested that miR‑654‑5p facilitated ECM degradation by increasing the expression levels of MMP‑3, MMP‑9 and MMP‑13, as well as decreasing collagen I, collagen II, SOX9 and aggrecan expression by inhibiting autophagy. Furthermore, autophagy‑related gene 7 (ATG7) was verified as a direct downstream target gene of miR‑654‑5p. miR‑654‑5p could bind to the 3' untranslated region of ATG7 to inhibit its mRNA expression and further reduce its translation. Notably, ATG7 knockdown abrogated the effects of the miR‑654‑5p inhibitor on ECM degradation and autophagy regulation. Furthermore, miR‑654‑5p inhibited autophagy in NP cells by increasing the protein expression levels of phosphorylated (p)‑PI3K, p‑AKT and p‑mTOR in an ATG7‑dependent manner. In conclusion, the results of the present study revealed that miR‑654‑5p may enhance ECM degradation via inhibition of autophagy by targeting ATG7 to activate the PI3K/AKT/mTOR signaling pathway. These findings may provide novel insights into the treatment of IDD.
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View References

1 

Clouet J, Fusellier M, Camus A, Le Visage C and Guicheux J: Intervertebral disc regeneration: From cell therapy to the development of novel bioinspired endogenous repair strategies. Adv Drug Deliv Rev. 146:306–324. 2019. View Article : Google Scholar : PubMed/NCBI

2 

Karran EL, McAuley JH, Traeger AC, Hillier SL, Grabherr L, Russek LN and Moseley GL: Can screening instruments accurately determine poor outcome risk in adults with recent onset low back pain? A systematic review and meta-analysis. BMC Med. 15:132017. View Article : Google Scholar : PubMed/NCBI

3 

Wang WJ, Yu XH, Wang C, Yang W, He WS, Zhang SJ, Yan YG and Zhang J: MMPs and ADAMTSs in intervertebral disc degeneration. Clin Chim Acta. 448:238–246. 2015. View Article : Google Scholar : PubMed/NCBI

4 

Evangelatos G, Fragoulis GE, Koulouri V and Lambrou GI: MicroRNAs in rheumatoid arthritis: From pathogenesis to clinical impact. Autoimmun Rev. 18:1023912019. View Article : Google Scholar : PubMed/NCBI

5 

Beermann J, Piccoli MT, Viereck J and Thum T: Non-coding RNAs in development and disease: Background, mechanisms, and therapeutic approaches. Physiol Rev. 96:1297–1325. 2016. View Article : Google Scholar : PubMed/NCBI

6 

Zhou X, Chen L, Grad S, Alini M, Pan H, Yang D, Zhen W, Li Z, Huang S and Peng S: The roles and perspectives of microRNAs as biomarkers for intervertebral disc degeneration. J Tissue Eng Regen Med. 11:3481–3487. 2017. View Article : Google Scholar : PubMed/NCBI

7 

Makki MS and Haqqi TM: MiR-139 modulates MCPIP1/IL-6 expression and induces apoptosis in human OA chondrocytes. Exp Mol Med. 47:e1892015. View Article : Google Scholar : PubMed/NCBI

8 

Huang Z, Pang G, Huang YG and Li C: MiR-133 inhibits proliferation and promotes apoptosis by targeting LASP1 in lupus nephritis. Exp Mol Pathol. 114:1043842020. View Article : Google Scholar : PubMed/NCBI

9 

Wang J, Liu X, Sun B, Du W, Zheng Y and Sun Y: Upregulated miR-154 promotes ECM degradation in intervertebral disc degeneration. J Cell Biochem. 2019.(Epub ahead of print).

10 

Zhu W, Li L and Li D: Rs11655237 polymorphism of LINC00673 affects the prognosis of cervical cancer by interfering with the interaction between LINC00673 and microRNA-1231. J Cell Physiol. 235:8155–8166. 2020. View Article : Google Scholar : PubMed/NCBI

11 

Majem B, Parrilla A, Jiménez C, Suárez-Cabrera L, Barber M, Marín A, Castellví J, Tamayo G, Moreno-Bueno G, Ponce J, et al: MicroRNA-654-5p suppresses ovarian cancer development impacting on MYC, WNT and AKT pathways. Oncogene. 38:6035–6050. 2019. View Article : Google Scholar : PubMed/NCBI

12 

Lu M, Wang C, Chen W, Mao C and Wang J: MiR-654-5p Targets GRAP to promote proliferation, metastasis, and chemoresistance of oral squamous cell carcinoma through Ras/MAPK Signaling. DNA Cell Biol. 37:381–388. 2018. View Article : Google Scholar : PubMed/NCBI

13 

Pu M, Chen J, Tao Z, Miao L, Qi X, Wang Y and Ren J: Regulatory network of miRNA on its target: Coordination between transcriptional and post-transcriptional regulation of gene expression. Cell Mol Life Sci. 76:441–451. 2019. View Article : Google Scholar : PubMed/NCBI

14 

Liu Y, Zhang Y, Liu P, Bai H, Li X, Xiao J, Yuan Q, Geng S, Yin H, Zhang H, et al: MicroRNA-128 knockout inhibits the development of Alzheimer's disease by targeting PPARγ in mouse models. Eur J Pharmacol. 843:134–144. 2019. View Article : Google Scholar : PubMed/NCBI

15 

Yang H, Liao D, Tong L, Zhong L and Wu K: MiR-373 exacerbates renal injury and fibrosis via NF-κB/MatrixMetalloproteinase-9 signaling by targeting Sirtuin1. Genomics. 111:786–792. 2019. View Article : Google Scholar : PubMed/NCBI

16 

Yang W and Sun P: Downregulation of microRNA-129-5p increases the risk of intervertebral disc degeneration by promoting the apoptosis of nucleus pulposus cells via targeting BMP2. J Cell Biochem. 120:19684–19690. 2019. View Article : Google Scholar : PubMed/NCBI

17 

Tan YY, Xu XY, Wang JF, Zhang CW and Zhang SC: MiR-654-5p attenuates breast cancer progression by targeting EPSTI1. Am J Cancer Res. 6:522–532. 2016.PubMed/NCBI

18 

Pfirrmann CW, Metzdorf A, Zanetti M, Hodler J and Boos N: Magnetic resonance classification of lumbar intervertebral disc degeneration. Spine (Phila Pa 1976). 26:1873–1878. 2001. View Article : Google Scholar : PubMed/NCBI

19 

World Medical Association: World medical association declaration of Helsinki: Ethical principles for medical research involving human subjects. JAMA. 310:2191–2194. 2013. View Article : Google Scholar

20 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

21 

Hochberg Y and Benjamini Y: More powerful procedures for multiple significance testing. Stat Med. 9:811–818. 1990. View Article : Google Scholar : PubMed/NCBI

22 

Feng C, Liu H, Yang Y, Huang B and Zhou Y: Growth and differentiation factor-5 contributes to the structural and functional maintenance of the intervertebral disc. Cell Physiol Biochem. 35:1–16. 2015. View Article : Google Scholar : PubMed/NCBI

23 

Feng C, Liu H, Yang M, Zhang Y, Huang B and Zhou Y: Disc cell senescence in intervertebral disc degeneration: Causes and molecular pathways. Cell Cycle. 15:1674–1684. 2016. View Article : Google Scholar : PubMed/NCBI

24 

Li ZY, Wang XL, Dang Y, Zhu XZ, Zhang YH, Cai BX and Zheng L: Long non-coding RNA UCA1 promotes the progression of paclitaxel resistance in ovarian cancer by regulating the miR-654-5p/SIK2 axis. Eur Rev Med Pharmacol Sci. 24:591–603. 2020.PubMed/NCBI

25 

Le Maitre CL, Pockert A, Buttle DJ, Freemont AJ and Hoyland JA: Matrix synthesis and degradation in human intervertebral disc degeneration. Biochem Soc Trans. 35:652–655. 2007. View Article : Google Scholar : PubMed/NCBI

26 

Vo NV, Hartman RA, Yurube T, Jacobs LJ, Sowa GA and Kang JD: Expression and regulation of metalloproteinases and their inhibitors in intervertebral disc aging and degeneration. Spine J. 13:331–341. 2013. View Article : Google Scholar : PubMed/NCBI

27 

Lippai M and Szatmari Z: Autophagy-from molecular mechanisms to clinical relevance. Cell Biol Toxicol. 33:145–168. 2017. View Article : Google Scholar : PubMed/NCBI

28 

Jiang W, Zhang X, Hao J, Shen J, Fang J, Dong W, Wang D, Zhang X, Shui W, Luo Y, et al: SIRT1 protects against apoptosis by promoting autophagy in degenerative human disc nucleus pulposus cells. Sci Rep. 4:74562014. View Article : Google Scholar : PubMed/NCBI

29 

Wang XH, Zhu L, Hong X, Wang YT, Wang F, Bao JP, Xie XH, Liu L and Wu XT: Resveratrol attenuated TNF-α-induced MMP-3 expression in human nucleus pulposus cells by activating autophagy via AMPK/SIRT1 signaling pathway. Exp Biol Med (Maywood). 241:848–853. 2016. View Article : Google Scholar : PubMed/NCBI

30 

Xu K, Chen W, Wang X, Peng Y, Liang A, Huang D, Li C and Ye W: Autophagy attenuates the catabolic effect during inflammatory conditions in nucleus pulposus cells, as sustained by NF-κB and JNK inhibition. Int J Mol Med. 36:661–668. 2015. View Article : Google Scholar : PubMed/NCBI

31 

Akkoc Y and Gozuacik D: MicroRNAs as major regulators of the autophagy pathway. Biochim Biophys Acta Mol Cell Res. 1867:1186622020. View Article : Google Scholar : PubMed/NCBI

32 

Gaviraghi M, Vivori C, Pareja Sanchez Y, Invernizzi F, Cattaneo A, Santoliquido BM, Frenquelli M, Segalla S, Bachi A, Doglioni C, et al: Tumor suppressor PNRC1 blocks rRNA maturation by recruiting the decapping complex to the nucleolus. EMBO J. 37:e991792018. View Article : Google Scholar : PubMed/NCBI

33 

Chen DY, Chen YM, Lin CF, Lo CM, Liu HJ and Liao TL: MicroRNA-889 inhibits autophagy to maintain mycobacterial survival in patients with latent tuberculosis infection by targeting TWEAK. mBio. 11:e03045–19. 2020. View Article : Google Scholar

34 

Wang WJ, Yang W, Ouyang ZH, Xue JB, Li XL, Zhang J, He WS, Chen WK, Yan YG and Wang C: MiR-21 promotes ECM degradation through inhibiting autophagy via the PTEN/akt/mTOR signaling pathway in human degenerated NP cells. Biomed Pharmacother. 99:725–734. 2018. View Article : Google Scholar : PubMed/NCBI

35 

Xiong J: Atg7 in development and disease: Panacea or Pandora's Box? Protein Cell. 6:722–734. 2015. View Article : Google Scholar : PubMed/NCBI

36 

Yu K, Li N, Cheng Q, Zheng J, Zhu M, Bao S, Chen M and Shi G: MiR-96-5p prevents hepatic stellate cell activation by inhibiting autophagy via ATG7. J Mol Med (Berl). 96:65–74. 2018. View Article : Google Scholar : PubMed/NCBI

37 

Wang C, Zhang ZZ, Yang W, Ouyang ZH, Xue JB, Li XL, Zhang J, Chen WK, Yan YG and Wang WJ: MiR-210 facilitates ECM degradation by suppressing autophagy via silencing of ATG7 in human degenerated NP cells. Biomed Pharmacother. 93:470–479. 2017. View Article : Google Scholar : PubMed/NCBI

38 

Zhang H, Zhang X and Zhang J: MiR-129-5p inhibits autophagy and apoptosis of H9c2 cells induced by hydrogen peroxide via the PI3K/AKT/mTOR signaling pathway by targeting ATG14. Biochem Biophys Res Commun. 506:272–277. 2018. View Article : Google Scholar : PubMed/NCBI

39 

He W and Cheng Y: Inhibition of miR-20 promotes proliferation and autophagy in articular chondrocytes by PI3K/AKT/mTOR signaling pathway. Biomed Pharmacother. 97:607–615. 2018. View Article : Google Scholar : PubMed/NCBI

40 

Zhang Y, Kwok-Shing Ng P, Kucherlapati M, Chen F, Liu Y, Tsang YH, de Velasco G, Jeong KJ, Akbani R, Hadjipanayis A, et al: A pan-cancer proteogenomic atlas of PI3K/AKT/mTOR pathway alterations. Cancer Cell. 31:820–832.e3. 2017. View Article : Google Scholar : PubMed/NCBI

41 

Zhao D, Zhang S, Wang X, Gao D, Liu J, Cao K, Chen L, Liu R, Liu J and Long J: ATG7 regulates hepatic Akt phosphorylation through the c-JUN/PTEN pathway in high fat diet-induced metabolic disorder. FASEB J. 33:14296–14306. 2019. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Wang S, Guo Y, Zhang X and Wang C: miR‑654‑5p inhibits autophagy by targeting ATG7 via mTOR signaling in intervertebral disc degeneration. Mol Med Rep 23: 444, 2021.
APA
Wang, S., Guo, Y., Zhang, X., & Wang, C. (2021). miR‑654‑5p inhibits autophagy by targeting ATG7 via mTOR signaling in intervertebral disc degeneration. Molecular Medicine Reports, 23, 444. https://doi.org/10.3892/mmr.2021.12083
MLA
Wang, S., Guo, Y., Zhang, X., Wang, C."miR‑654‑5p inhibits autophagy by targeting ATG7 via mTOR signaling in intervertebral disc degeneration". Molecular Medicine Reports 23.6 (2021): 444.
Chicago
Wang, S., Guo, Y., Zhang, X., Wang, C."miR‑654‑5p inhibits autophagy by targeting ATG7 via mTOR signaling in intervertebral disc degeneration". Molecular Medicine Reports 23, no. 6 (2021): 444. https://doi.org/10.3892/mmr.2021.12083
Copy and paste a formatted citation
x
Spandidos Publications style
Wang S, Guo Y, Zhang X and Wang C: miR‑654‑5p inhibits autophagy by targeting ATG7 via mTOR signaling in intervertebral disc degeneration. Mol Med Rep 23: 444, 2021.
APA
Wang, S., Guo, Y., Zhang, X., & Wang, C. (2021). miR‑654‑5p inhibits autophagy by targeting ATG7 via mTOR signaling in intervertebral disc degeneration. Molecular Medicine Reports, 23, 444. https://doi.org/10.3892/mmr.2021.12083
MLA
Wang, S., Guo, Y., Zhang, X., Wang, C."miR‑654‑5p inhibits autophagy by targeting ATG7 via mTOR signaling in intervertebral disc degeneration". Molecular Medicine Reports 23.6 (2021): 444.
Chicago
Wang, S., Guo, Y., Zhang, X., Wang, C."miR‑654‑5p inhibits autophagy by targeting ATG7 via mTOR signaling in intervertebral disc degeneration". Molecular Medicine Reports 23, no. 6 (2021): 444. https://doi.org/10.3892/mmr.2021.12083
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