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Article

Salvianolic acid B inhibits myofibroblast differentiation and extracellular matrix accumulation in nasal polyp fibroblasts via the TGF-β1 signaling pathway

  • Authors:
    • Zhenfeng Sun
    • Dan Luo
    • Sang Wang
    • Ruofei Huang
    • Pin Dong
  • View Affiliations / Copyright

    Affiliations: Department of Otolaryngology, Head and Neck Surgery, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 201620, P.R. China
  • Article Number: 478
    |
    Published online on: April 22, 2021
       https://doi.org/10.3892/mmr.2021.12117
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Abstract

In the process of nasal tissue remodeling, nasal fibroblasts serve an important role via myofibroblast differentiation and the production of extracellular matrix (ECM). Nasal fibroblast abnormalities can lead to conditions such as chronic rhinosinusitis. Salvianolic acid B (Sal B), a water-soluble active pharmaceutical compound extract from the root of the traditional Chinese medicine Salvia miltiorrhiza, displays antioxidative, antiproliferative and antifibrosis properties. The present study aimed to investigate the mechanism underlying the effects of Sal B on nasal polyp fibroblast (NPF) myofibroblast differentiation and ECM accumulation. Primary NPFs were obtained from nasal polyps of patients with chronic sinusitis. The proliferative and cytotoxic effects of Sal B on NPFs were evaluated by performing the Cell Counting Kit-8 assay. The Transwell assay was conducted to assess cell migration. α-smooth muscle actin (α-SMA), TGF-β1 receptor (TβR)-I, TβR-II, Smad2/3 mRNA and protein expression levels and (p)-Smad2/3 phosphorylation levels were measured via reverse transcription-quantitative PCR and western blotting, respectively. Type III collagen and fibronectin levels were analyzed by ELISA. The results indicated that Sal B significantly downregulated TGF-β1-induced α-SMA, fibronectin and collagen III expression levels in NPFs. Similarly, Sal B significantly decreased TGF-β1-induced TβR-I, TβR-II, p-Smad2/3, MMP-2 and MMP-9 mRNA and protein expression levels in NPFs. Furthermore, Sal B significantly decreased TGF-β1-induced NPF migration. Therefore, the present study indicated that Sal B inhibited myofibroblast differentiation and ECM accumulation in nasal fibroblasts, suggesting that Sal B may inhibit nasal polyp formation via certain mechanisms.
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Copy and paste a formatted citation
Spandidos Publications style
Sun Z, Luo D, Wang S, Huang R and Dong P: Salvianolic acid B inhibits myofibroblast differentiation and extracellular matrix accumulation in nasal polyp fibroblasts via the TGF-β1 signaling pathway. Mol Med Rep 23: 478, 2021.
APA
Sun, Z., Luo, D., Wang, S., Huang, R., & Dong, P. (2021). Salvianolic acid B inhibits myofibroblast differentiation and extracellular matrix accumulation in nasal polyp fibroblasts via the TGF-β1 signaling pathway. Molecular Medicine Reports, 23, 478. https://doi.org/10.3892/mmr.2021.12117
MLA
Sun, Z., Luo, D., Wang, S., Huang, R., Dong, P."Salvianolic acid B inhibits myofibroblast differentiation and extracellular matrix accumulation in nasal polyp fibroblasts via the TGF-β1 signaling pathway". Molecular Medicine Reports 23.6 (2021): 478.
Chicago
Sun, Z., Luo, D., Wang, S., Huang, R., Dong, P."Salvianolic acid B inhibits myofibroblast differentiation and extracellular matrix accumulation in nasal polyp fibroblasts via the TGF-β1 signaling pathway". Molecular Medicine Reports 23, no. 6 (2021): 478. https://doi.org/10.3892/mmr.2021.12117
Copy and paste a formatted citation
x
Spandidos Publications style
Sun Z, Luo D, Wang S, Huang R and Dong P: Salvianolic acid B inhibits myofibroblast differentiation and extracellular matrix accumulation in nasal polyp fibroblasts via the TGF-β1 signaling pathway. Mol Med Rep 23: 478, 2021.
APA
Sun, Z., Luo, D., Wang, S., Huang, R., & Dong, P. (2021). Salvianolic acid B inhibits myofibroblast differentiation and extracellular matrix accumulation in nasal polyp fibroblasts via the TGF-β1 signaling pathway. Molecular Medicine Reports, 23, 478. https://doi.org/10.3892/mmr.2021.12117
MLA
Sun, Z., Luo, D., Wang, S., Huang, R., Dong, P."Salvianolic acid B inhibits myofibroblast differentiation and extracellular matrix accumulation in nasal polyp fibroblasts via the TGF-β1 signaling pathway". Molecular Medicine Reports 23.6 (2021): 478.
Chicago
Sun, Z., Luo, D., Wang, S., Huang, R., Dong, P."Salvianolic acid B inhibits myofibroblast differentiation and extracellular matrix accumulation in nasal polyp fibroblasts via the TGF-β1 signaling pathway". Molecular Medicine Reports 23, no. 6 (2021): 478. https://doi.org/10.3892/mmr.2021.12117
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