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Role of P2X7R in eosinophilic and non‑eosinophilic chronic rhinosinusitis with nasal polyps

  • Authors:
    • Yan Wang
    • Shan Chen
    • Wenwen Wang
    • Jianjun Chen
    • Weijia Kong
    • Yanjun Wang
  • View Affiliations / Copyright

    Affiliations: Department of Otorhinolaryngology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430022, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 521
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    Published online on: May 20, 2021
       https://doi.org/10.3892/mmr.2021.12160
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Abstract

Chronic rhinosinusitis with nasal polyps (CRSwNP) is an inflammation‑mediated disease of the nasal mucosa. P2X7R has been reported to be a potential biomarker for inflammation. The aim of the present study was to explore the role of P2X7R in CRSwNP, and the interaction between P2X7R and the NLRP3 inflammasome in the development of CRSwNP. Firstly, the expression profiles of P2X7R in nasal mucosa were investigated using western blotting (WB), polymerase chain reaction (PCR) and immunofluorescence (IF) staining. Next, the effect of inflammatory stimulation with lipopolysaccharides (LPS) combined with 2'(3')‑O‑(4‑benzoylbenzoyl) adenosine 5'‑triphosphate triethylammonium salt (BzATP) on primary human nasal epithelial cells (HNECs) was determined. Then, the therapeutic effect of the selective P2X7R antagonist, A740003, on P3X7R, NOD‑like receptor pyrin domain containing 3 (NLRP3) inflammasome and IL‑1β alterations in HNECs was explored using enzyme‑linked immunosorbent assay, WB and PCR. It was found that P2X7R was overexpressed in CRSwNP, especially in eosinophilic CRSwNP, the expression of P2X7R, NLRP3 and IL‑1β were upregulated in HNECs after induction by LPS combined with BzATP; but the expression of NLRP3 and IL‑1β were downregulated after stimulation with A740003. The aforementioned results indicate that P2X7R‑mediated NLRP3 inflammasome activation may have a role in the pathogenesis of CRSwNP.
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Copy and paste a formatted citation
Spandidos Publications style
Wang Y, Chen S, Wang W, Chen J, Kong W and Wang Y: Role of P2X7R in eosinophilic and non‑eosinophilic chronic rhinosinusitis with nasal polyps. Mol Med Rep 24: 521, 2021.
APA
Wang, Y., Chen, S., Wang, W., Chen, J., Kong, W., & Wang, Y. (2021). Role of P2X7R in eosinophilic and non‑eosinophilic chronic rhinosinusitis with nasal polyps. Molecular Medicine Reports, 24, 521. https://doi.org/10.3892/mmr.2021.12160
MLA
Wang, Y., Chen, S., Wang, W., Chen, J., Kong, W., Wang, Y."Role of P2X7R in eosinophilic and non‑eosinophilic chronic rhinosinusitis with nasal polyps". Molecular Medicine Reports 24.1 (2021): 521.
Chicago
Wang, Y., Chen, S., Wang, W., Chen, J., Kong, W., Wang, Y."Role of P2X7R in eosinophilic and non‑eosinophilic chronic rhinosinusitis with nasal polyps". Molecular Medicine Reports 24, no. 1 (2021): 521. https://doi.org/10.3892/mmr.2021.12160
Copy and paste a formatted citation
x
Spandidos Publications style
Wang Y, Chen S, Wang W, Chen J, Kong W and Wang Y: Role of P2X7R in eosinophilic and non‑eosinophilic chronic rhinosinusitis with nasal polyps. Mol Med Rep 24: 521, 2021.
APA
Wang, Y., Chen, S., Wang, W., Chen, J., Kong, W., & Wang, Y. (2021). Role of P2X7R in eosinophilic and non‑eosinophilic chronic rhinosinusitis with nasal polyps. Molecular Medicine Reports, 24, 521. https://doi.org/10.3892/mmr.2021.12160
MLA
Wang, Y., Chen, S., Wang, W., Chen, J., Kong, W., Wang, Y."Role of P2X7R in eosinophilic and non‑eosinophilic chronic rhinosinusitis with nasal polyps". Molecular Medicine Reports 24.1 (2021): 521.
Chicago
Wang, Y., Chen, S., Wang, W., Chen, J., Kong, W., Wang, Y."Role of P2X7R in eosinophilic and non‑eosinophilic chronic rhinosinusitis with nasal polyps". Molecular Medicine Reports 24, no. 1 (2021): 521. https://doi.org/10.3892/mmr.2021.12160
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