Open Access

Effects of miR‑210‑3p on the erythroid differentiation of K562 cells under hypoxia

  • Authors:
    • Caiyan Hu
    • Yupeng Yan
    • Chengbing Fu
    • Jin Ding
    • Tiantian Li
    • Shuqiong Wang
    • Liu Fang
  • View Affiliations

  • Published online on: June 7, 2021     https://doi.org/10.3892/mmr.2021.12202
  • Article Number: 563
  • Copyright: © Hu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

GATA binding protein 1 (GATA‑1) is one of the most important hematopoietic transcription factors in the production of blood cells, such as platelets, eosinophils, mast cells and erythrocytes. GATA‑1 regulates the participation of microRNA (miRNAs/miRs) in erythroid differentiation under normoxia. However, GATA‑1 expression and the regulation of miR‑210‑3p in the context of erythroid differentiation under hypoxia remain unknown. The present study examined the expression levels of GATA‑1 and miR‑210‑3p in the model of erythroid differentiation in K562 cells under hypoxia, and determined the effects of GATA‑1, miR‑210‑3p and SMAD2 on erythroid differentiation through lentivirus transfection experiments. The present study detected increased GATA‑1 expression under hypoxia. Moreover, miR‑210‑3p was identified as a positive regulator of erythroid differentiation, which was upregulated both during erythroid differentiation and in GATA‑1 overexpression experiments under hypoxia. Importantly, in the K562 cell model of erythroid differentiation under hypoxia, miR‑210‑3p was upregulated in a GATA‑1‑dependent manner. Using a double luciferase reporter assay, miR‑210‑3p was identified as a downstream target of GATA‑1‑mediated regulation of erythropoiesis. Gain‑ or loss‑of‑function analysis of miR‑210‑3p identified its importance in erythroid differentiation. Furthermore, it was found that SMAD2 may be a downstream target gene for miR‑210‑3p. Bioinformatics predictions suggested that SMAD2 mediated miR‑210‑3p‑induced regulation of erythroid differentiation. Collectively, the present study provides novel insights into the miRNA regulation of erythroid differentiation.
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August-2021
Volume 24 Issue 2

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Hu C, Yan Y, Fu C, Ding J, Li T, Wang S and Fang L: Effects of miR‑210‑3p on the erythroid differentiation of K562 cells under hypoxia. Mol Med Rep 24: 563, 2021
APA
Hu, C., Yan, Y., Fu, C., Ding, J., Li, T., Wang, S., & Fang, L. (2021). Effects of miR‑210‑3p on the erythroid differentiation of K562 cells under hypoxia. Molecular Medicine Reports, 24, 563. https://doi.org/10.3892/mmr.2021.12202
MLA
Hu, C., Yan, Y., Fu, C., Ding, J., Li, T., Wang, S., Fang, L."Effects of miR‑210‑3p on the erythroid differentiation of K562 cells under hypoxia". Molecular Medicine Reports 24.2 (2021): 563.
Chicago
Hu, C., Yan, Y., Fu, C., Ding, J., Li, T., Wang, S., Fang, L."Effects of miR‑210‑3p on the erythroid differentiation of K562 cells under hypoxia". Molecular Medicine Reports 24, no. 2 (2021): 563. https://doi.org/10.3892/mmr.2021.12202