Open Access

Inhibition of lncRNA SNHG8 plays a protective role in hypoxia-ischemia-reoxygenation-induced myocardial injury by regulating miR-335 and RASA1 expression

  • Authors:
    • Yanfeng Liu
    • Ping Zhou
    • Fengxiao Wang
    • Xuehong Zhang
    • Dongmei Yang
    • Lang Hong
    • Dongyun Ruan
  • View Affiliations

  • Published online on: June 22, 2021     https://doi.org/10.3892/mmr.2021.12236
  • Article Number: 597
  • Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Long non‑coding (lnc)RNAs serve a role in a number of diseases, including different types of cancer and acute myocardial infarction. The aim of the present study was to investigate the protective role of lncRNA small nucleolar RNA host gene 8 (SNHG8) in hypoxia‑ischemia‑reoxygenation (HI/R)‑induced myocardial injury and its potential mechanism of action. Cell viability, proliferation, creatine kinase myocardial band, cell apoptosis and protein expression levels were determined by Cell Counting Kit‑8 assay, EdU assay, ELISA, flow cytometry and western blotting, respectively. The association between SNHG8 and microRNA (miR)‑335 was confirmed using a dual‑luciferase reporter gene assay. The effects of the miR‑335 inhibitor transfections had on increasing apoptosis and decreasing H9C2 cell viability were reversed in cells co‑transfected with SNHG8 small interfering (si)RNA. Furthermore, it was found that miR‑335 could regulate RAS p21 protein activator 1 (RASA1) expression and that transfection with SNHG8 siRNA downregulated RASA1 expression. Silencing of RASA1 protected against HI/R‑induced H9C2 cell injury. However, SNHG8 siRNA did not further reduce apoptosis, demonstrating that SNHG8 may act through RASA1, and RASA1 may mediate the protection of SNHG8 siRNA in HI/R myocardial injury. Thus, inhibition of lncRNA SNHG8 alleviated HI/R‑induced myocardial damage by regulating miR‑335 and RASA1.
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August-2021
Volume 24 Issue 2

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Liu Y, Zhou P, Wang F, Zhang X, Yang D, Hong L and Ruan D: Inhibition of lncRNA <em>SNHG8</em> plays a protective role in hypoxia-ischemia-reoxygenation-induced myocardial injury by regulating miR-335 and RASA1 expression. Mol Med Rep 24: 597, 2021
APA
Liu, Y., Zhou, P., Wang, F., Zhang, X., Yang, D., Hong, L., & Ruan, D. (2021). Inhibition of lncRNA <em>SNHG8</em> plays a protective role in hypoxia-ischemia-reoxygenation-induced myocardial injury by regulating miR-335 and RASA1 expression. Molecular Medicine Reports, 24, 597. https://doi.org/10.3892/mmr.2021.12236
MLA
Liu, Y., Zhou, P., Wang, F., Zhang, X., Yang, D., Hong, L., Ruan, D."Inhibition of lncRNA <em>SNHG8</em> plays a protective role in hypoxia-ischemia-reoxygenation-induced myocardial injury by regulating miR-335 and RASA1 expression". Molecular Medicine Reports 24.2 (2021): 597.
Chicago
Liu, Y., Zhou, P., Wang, F., Zhang, X., Yang, D., Hong, L., Ruan, D."Inhibition of lncRNA <em>SNHG8</em> plays a protective role in hypoxia-ischemia-reoxygenation-induced myocardial injury by regulating miR-335 and RASA1 expression". Molecular Medicine Reports 24, no. 2 (2021): 597. https://doi.org/10.3892/mmr.2021.12236