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Effects of sterol derivatives in cationic liposomes on biodistribution and gene-knockdown in the lungs of mice systemically injected with siRNA lipoplexes

  • Authors:
    • Yoshiyuki Hattori
    • Hiromu Saito
    • Teruaki Oku
    • Kei-Ichi Ozaki
  • View Affiliations / Copyright

    Affiliations: Department of Molecular Pharmaceutics, Hoshi University, Tokyo 142-8501, Japan, Department of Microbiology, Hoshi University, Tokyo 142-8501, Japan, Department of Molecular Pathology, Faculty of Pharmaceutical Sciences, Doshisha Women's College of Liberal Arts, Kyotanabe, Kyoto 610-0395, Japan
    Copyright: © Hattori et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 598
    |
    Published online on: June 23, 2021
       https://doi.org/10.3892/mmr.2021.12237
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Abstract

Cationic liposomes can be intravenously injected to deliver short interfering (si)RNAs into the lungs. The present study investigated the effects of sterol derivatives in systemically injected siRNA/cationic liposome complexes (siRNA lipoplexes) on gene‑knockdown in the lungs of mice. Cationic liposomes composed of 1,2‑dioleoyl‑3‑trimethylammonium‑propane or dimethyldioctadecylammonium bromide (DDAB) were prepared as a cationic lipid, with sterol derivatives such as cholesterol (Chol), β‑sitosterol, ergosterol (Ergo) or stigmasterol as a neutral helper lipid. Transfected liposomal formulations composed of DDAB/Chol or DDAB/Ergo did not suppress the expression of the luciferase gene in LLC‑Luc and Colon 26‑Luc cells in vitro, whereas other formulations induced moderate gene‑silencing. The systemic injection of siRNA lipoplexes formulated with Chol or Ergo into mice resulted in abundant siRNA accumulation in the lungs. In comparison, systemically injected DDAB/Chol or DDAB/Ergo lipoplexes of Tie2 siRNA effectively increased the suppression of the Tie2 mRNA expression in the lungs of mice. These findings indicated that DDAB/Chol and DDAB/Ergo liposomes could function as vectors for siRNA delivery to the lungs.
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Copy and paste a formatted citation
Spandidos Publications style
Hattori Y, Saito H, Oku T and Ozaki K: Effects of sterol derivatives in cationic liposomes on biodistribution and gene-knockdown in the lungs of mice systemically injected with siRNA lipoplexes. Mol Med Rep 24: 598, 2021.
APA
Hattori, Y., Saito, H., Oku, T., & Ozaki, K. (2021). Effects of sterol derivatives in cationic liposomes on biodistribution and gene-knockdown in the lungs of mice systemically injected with siRNA lipoplexes. Molecular Medicine Reports, 24, 598. https://doi.org/10.3892/mmr.2021.12237
MLA
Hattori, Y., Saito, H., Oku, T., Ozaki, K."Effects of sterol derivatives in cationic liposomes on biodistribution and gene-knockdown in the lungs of mice systemically injected with siRNA lipoplexes". Molecular Medicine Reports 24.2 (2021): 598.
Chicago
Hattori, Y., Saito, H., Oku, T., Ozaki, K."Effects of sterol derivatives in cationic liposomes on biodistribution and gene-knockdown in the lungs of mice systemically injected with siRNA lipoplexes". Molecular Medicine Reports 24, no. 2 (2021): 598. https://doi.org/10.3892/mmr.2021.12237
Copy and paste a formatted citation
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Spandidos Publications style
Hattori Y, Saito H, Oku T and Ozaki K: Effects of sterol derivatives in cationic liposomes on biodistribution and gene-knockdown in the lungs of mice systemically injected with siRNA lipoplexes. Mol Med Rep 24: 598, 2021.
APA
Hattori, Y., Saito, H., Oku, T., & Ozaki, K. (2021). Effects of sterol derivatives in cationic liposomes on biodistribution and gene-knockdown in the lungs of mice systemically injected with siRNA lipoplexes. Molecular Medicine Reports, 24, 598. https://doi.org/10.3892/mmr.2021.12237
MLA
Hattori, Y., Saito, H., Oku, T., Ozaki, K."Effects of sterol derivatives in cationic liposomes on biodistribution and gene-knockdown in the lungs of mice systemically injected with siRNA lipoplexes". Molecular Medicine Reports 24.2 (2021): 598.
Chicago
Hattori, Y., Saito, H., Oku, T., Ozaki, K."Effects of sterol derivatives in cationic liposomes on biodistribution and gene-knockdown in the lungs of mice systemically injected with siRNA lipoplexes". Molecular Medicine Reports 24, no. 2 (2021): 598. https://doi.org/10.3892/mmr.2021.12237
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