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Article Open Access

Circular RNA profiling reveals a potential role of hsa_circ_IPCEF1 in papillary thyroid carcinoma

  • Authors:
    • Min Guo
    • Yushuang Sun
    • Junzhu Ding
    • Yong Li
    • Sihan Yang
    • Yanna Zhao
    • Xin Jin
    • Shan-Shan Li
  • View Affiliations / Copyright

    Affiliations: Department of Endocrinology, The Fourth Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China, College of Pharmacy, Harbin Medical University, Harbin, Heilongjiang 150081, P.R. China, School of Medicine, Nankai University, Tianjin 300071, P.R. China
    Copyright: © Guo et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 603
    |
    Published online on: June 24, 2021
       https://doi.org/10.3892/mmr.2021.12241
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Abstract

Circular RNAs (circRNAs) are a novel type of non‑coding RNAs that are expressed across species and are implicated in cellular biological processes, displaying dysregulated expression in various tumorigeneses. Therefore, circRNA deregulation could be a crucial event in thyroid carcinoma. The present study identified circRNA signatures in several patients with papillary thyroid carcinoma (PTC) to complement the understanding of PTC pathogenesis. Using microarray technology, the circRNA profiles in three pairs of PTC tumors and matching adjacent normal tissues were screened. Differentially expressed circRNAs were further validated by reverse transcription‑quantitative PCR in whole blood from 57 pairs of subjects. Bioinformatics data analyses including miRNA response element prediction, Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway, competing endogenous RNA and KEGG Orthology‑Based Annotation System analyses were performed to predict circRNA associations with cancer‑related putative downstream miRNAs and target genes. Receiver operating characteristic curves and the area under the curve (AUC) values were acquired to assess the performance of validated circRNAs in predicting potential associations with PTC. In total, 158 dysregulated circRNAs were identified in PTC tumors relative to adjacent normal tissues. Notably, one downregulated circRNA (hsa_circ_IPCEF1) showed the preferable predictive power (AUC=0.8010, P<0.0001) and interactions with four cancer‑related genes (CASR, CDC25B, NFκB1 and SHOC2). From these analyses, one PTC‑related miRNA (hsa‑miR‑3619‑5p) was identified as a potential target for hsa_circ_IPCEF1 sponging, indicating the hsa_circ_IPCEF1/hsa‑miR‑3619‑5p axis in pathogenesis.
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Copy and paste a formatted citation
Spandidos Publications style
Guo M, Sun Y, Ding J, Li Y, Yang S, Zhao Y, Jin X and Li S: Circular RNA profiling reveals a potential role of hsa_circ_IPCEF1 in papillary thyroid carcinoma. Mol Med Rep 24: 603, 2021.
APA
Guo, M., Sun, Y., Ding, J., Li, Y., Yang, S., Zhao, Y. ... Li, S. (2021). Circular RNA profiling reveals a potential role of hsa_circ_IPCEF1 in papillary thyroid carcinoma. Molecular Medicine Reports, 24, 603. https://doi.org/10.3892/mmr.2021.12241
MLA
Guo, M., Sun, Y., Ding, J., Li, Y., Yang, S., Zhao, Y., Jin, X., Li, S."Circular RNA profiling reveals a potential role of hsa_circ_IPCEF1 in papillary thyroid carcinoma". Molecular Medicine Reports 24.2 (2021): 603.
Chicago
Guo, M., Sun, Y., Ding, J., Li, Y., Yang, S., Zhao, Y., Jin, X., Li, S."Circular RNA profiling reveals a potential role of hsa_circ_IPCEF1 in papillary thyroid carcinoma". Molecular Medicine Reports 24, no. 2 (2021): 603. https://doi.org/10.3892/mmr.2021.12241
Copy and paste a formatted citation
x
Spandidos Publications style
Guo M, Sun Y, Ding J, Li Y, Yang S, Zhao Y, Jin X and Li S: Circular RNA profiling reveals a potential role of hsa_circ_IPCEF1 in papillary thyroid carcinoma. Mol Med Rep 24: 603, 2021.
APA
Guo, M., Sun, Y., Ding, J., Li, Y., Yang, S., Zhao, Y. ... Li, S. (2021). Circular RNA profiling reveals a potential role of hsa_circ_IPCEF1 in papillary thyroid carcinoma. Molecular Medicine Reports, 24, 603. https://doi.org/10.3892/mmr.2021.12241
MLA
Guo, M., Sun, Y., Ding, J., Li, Y., Yang, S., Zhao, Y., Jin, X., Li, S."Circular RNA profiling reveals a potential role of hsa_circ_IPCEF1 in papillary thyroid carcinoma". Molecular Medicine Reports 24.2 (2021): 603.
Chicago
Guo, M., Sun, Y., Ding, J., Li, Y., Yang, S., Zhao, Y., Jin, X., Li, S."Circular RNA profiling reveals a potential role of hsa_circ_IPCEF1 in papillary thyroid carcinoma". Molecular Medicine Reports 24, no. 2 (2021): 603. https://doi.org/10.3892/mmr.2021.12241
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