Anti‑angiogenic effect of mountain ginseng <em>in vitro</em> and <em>in vivo</em>: Comparison with farm‑cultivated ginseng

Kim,Jin Soo; Yoo,Jae-Myung; Park,Jong Eel; Kim,Jungeun; Kim,Sun-Gun; Seok,Young Mi; Son,Jun-Ho; Kim,Hyo Jung

doi:10.3892/mmr.2021.12254

August-2021 Volume 24 Issue 2

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August-2021 Volume 24 Issue 2

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Article

Anti‑angiogenic effect of mountain ginseng in vitro and in vivo: Comparison with farm‑cultivated ginseng

Authors:
- Jin Soo Kim
- Jae-Myung Yoo
- Jong Eel Park
- Jungeun Kim
- Sun-Gun Kim
- Young Mi Seok
- Jun-Ho Son
- Hyo Jung Kim
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Affiliations: Korean Medicine R&D Team 1, National Institute for Korean Medicine Development (NIKOM), Gyeongsan 38540, Republic of Korea, Unimed Pharmaceutical Inc., Seoul 05567, Republic of Korea
Article Number: 615
|
Published online on: June 29, 2021

https://doi.org/10.3892/mmr.2021.12254
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Abstract

Mountain ginseng (Panax ginseng) has been used for cancer patient therapy in Northeast Asia. Although it is well known that cancer cells are able to induce angiogenesis, the effect of mountain ginseng on angiogenesis is still unknown. In the present study, we investigated whether ethanolic extract of mountain ginseng (MGE) could inhibit angiogenesis in in vitro and in vivo models. In comparison with farm‑cultivated ginseng extract (FGE), MGE more strongly inhibited cell migration and formation of capillary‑like network within non‑cytotoxic ranges in SVEC4‑10 cells. In addition, MGE dose‑dependently suppressed Transwell cell migration of the cells. Moreover, MGE reduced the phosphorylation and expression of VEGF‑R2 as well as the phosphorylation of FAK, Src, Akt and ERK, the intermediate proteins in the VEGF‑R2 signaling cascade, in the cells. As expected, MGE dramatically decreased hemoglobin content in Matrigel plugs in mice. In conclusion, MGE possesses stronger anti‑angiogenic properties than FGE in vascular endothelial cells. Such effect of MGE is correlated with inhibition of activation of the VEGF‑R2 signaling pathway. Therefore, the novel features of MGE may be helpful for understanding its anticancer mechanism for the treatment of cancer patients.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Anti‑angiogenic effect of mountain ginseng in vitro and in vivo: Comparison with farm‑cultivated ginseng

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