Chemokine CCL20 promotes the paclitaxel resistance of CD44+CD117+ cells via the Notch1 signaling pathway in ovarian cancer
- Authors:
- Published online on: July 6, 2021 https://doi.org/10.3892/mmr.2021.12274
- Article Number: 635
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Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Abstract
Introduction
One of the most common lethal malignancies in women worldwide is ovarian cancer (1). Ovarian cancer is ranked as the 7th most prevalent female cancer type, as shown by a 2018 report, accounting for >180,000 deaths in China (2). Moreover, ~70% of ovarian cancer is diagnosed at an advanced stage due to silent symptoms, the hidden growth of the tumor and the lack of appropriate early diagnostic methods (3). Despite advancements in chemotherapy and surgery, the prognosis of patients with ovarian cancer has not changed significantly over the last few decades. Moreover, drug resistance has become a new challenge for the therapy of ovarian cancer (4). Thus, it is important to illustrate the mechanism of drug resistance and overcome this challenge for improving the therapy and prognosis of ovarian cancer.
The standard treatment for ovarian cancer is surgery combined with paclitaxel (PTX)- and platinum-based adjuvant chemotherapy (5). However, the first line obstruction in ovarian cancer treatment is PTX and platinum resistance (6). As indicated by the mechanism of the drug-resistant tumor, there may be a pool of self-renewing malignant cells, namely ovarian cancer stem-like cells, which could enhance the self-renewal, reproduction and drug resistance of tumors, thereby serving key roles in the initiation, diffusion, metastasis and recurrence of cancer (7,8). As shown in previous studies, a potential marker for ovarian stem-like cancer cells could be CD44, along with CD117 (9,10). Increased expression levels of CD44, CD117 and aldehyde dehydrogenase 1 family member A1 in SKOV3 cells are significantly correlated with the enhancing features of epithelial-mesenchymal transition (EMT), sphere formation, metastasis and multidrug resistance abilities (11,12). At present, the sensitivity of CD44+CD117+ cells to chemotherapy drugs, including PTX, remains unknown and needs to be further investigated. Moreover, in the drug resistance of ovarian cancer, the exact mechanism of cancer stem-like cells are yet to be fully elucidated.
C-C motif chemokine ligand 20 (CCL20), as a ligand of C-C motif chemokine receptor 6 (CCR6), serves a key role in the development of numerous cancer types, such as lung cancer, colorectal cancer and thyroid cancer (13,14). Moreover, the CCL20/CCR6 axis serves an important role in the process of drug resistance (15). As shown in a recent study, cisplatin can stimulate macrophages to secrete CCL20 and promote ovarian cancer cell migration via the CCL20/CCR6 axis (16). However, in ovarian cancer, the role of the CCL20/CCR6 axis and its mechanism remain unclear and require further examination.
In embryonic development and numerous cellular physiological processes, notch receptor (Notch) is involved in regulating cell proliferation, apoptosis and differentiation (17). As a member of the Notch family, Notch1 is implicated in multiple cancer types, such as breast cancer (especially triple-negative breast cancer), leukemias and brain tumors, among others (18,19). Notch1 is closely associated with numerous signaling pathways that are therapeutically involved in tumorigenesis, such as TLR4/NF-κB and PI3K/AKT (20). Together these impact apoptosis, proliferation, chemosensitivity, immune response and the population of cancer stem cells.
In the present study, a population of CD44+CD117+ subgroup cells was isolated from SKOV3 cells, followed by determining their stemness and drug resistance. Furthermore, the underlying mechanism of CD44+CD117+ cells in drug resistance and stemness maintenance was examined. The current study also provides novel insights into the mechanism and clinical therapy of PTX resistance in ovarian cancer.
Materials and methods
Cell culture and isolation
The human ovarian cancer cell line, SKOV-3, was purchased from The Shanghai Stem Cell Institute, and was cultured in DMEM (Invitrogen; Thermo Fisher Scientific, Inc.) supplemented with 10% FBS (Gibco; Thermo Fisher Scientific, Inc.) and 1% penicillin-streptomycin mixture (Sigma-Aldrich; Merck KGaA) at 37°C in a humidified atmosphere with 5% CO2.
CD44+CD117+ cells were then separated from SKOV-3 cells using the magnetic-activated cell sorting kit (Miltenyi Biotec GmbH) according to the manufacturer's instructions. The kit included CD44+ and CD117+ antibodies. CD44+ cells were sorted using a mouse anti-human CD44+ antibody combined with magnetic microbeads. After magnetic column depletion, among CD44+ SKOV3 cells, CD117+ cells were isolated using CD117+ antibody combined with magnetic microbeads. Moreover, among CD44− cells, the CD117+ subset was isolated using CD117+ antibody combined with magnetic microbeads. The CD44+CD117+ stem-like subsets and CD44−CD117− cells were harvested after magnetic column depletion and maintained in a DMEM/F12 medium containing 5 µg/ml insulin (Sigma-Aldrich; Merck KGaA), 10 ng/ml basic fibroblast growth factor (bFGF; Sigma-Aldrich; Merck KGaA), 20 ng/ml human recombinant epidermal growth factor (EGF; Sigma-Aldrich; Merck KGaA) and 0.5% FBS.
Construction of transient small interfering RNA (siRNA/si)Notch1 cell lines
According to GenBank (NM_017617.4), a siRNA sequence of human Notch1 was designed. The sequence was as follows: siNotch1-1 forward (F), 5′-AGUGGACAUCAGUACUGUA-3′ and reverse (R), 3′-UACAGUACUGAUGUCCACU-5′; siNotch1-2 F, 5′-AUGCGGGCAAGUGCAUCAACA-3′ and R, 3′-UGUUGAUGCACUUGCCCGCAU-5′; and siNotch1-3 F, 5′-GCUACACAGGGAGCAUGUGUA-3′ and R, 3′-TACACATGCTCCCTGTGTAGC-5′. The negative control (siNC) was designed as follows: F, 5′-UUCUCCGAACGUGUCACGUTT-3′ and R, 3′-ACGUGACACGUUCGGAGAATT-5′. All primers were synthesized and recombined to the plasmids (pcDNA3.1) by Shanghai GenePharma Co., Ltd.. After cells were inoculated overnight in a 6-well plate with 60% confluency, 20 µl siNotch1 or siNC recombinant were transfected into cells using Lipofectamine® 2000 (Invitrogen; Thermo Fisher Scientific, Inc.), according to the manufacturer's protocol at 37° and 5% CO2 in an incubator and for the following experiments 48 h later as indicated.
Xenograft and grouping
In total, 16 male nude mice (age, 6–8 weeks; weight, 25–30 g) were purchased from the Experimental Animal Center of the Shandong University and raised at the Experimental Animal Center. Mice were housed at 25±1°C with 12-h light/dark cycles, 50±5% humidity and free access to food and water. The mice were randomly divided into four groups: i) CD44+CD117+ + PTX (Beijing Solarbio Science & Technology Co., Ltd.) group (n=4); ii) CD44+CD117+ + PTX + CCL20 (Beijing Solarbio Science & Technology Co., Ltd.) group (n=4); iii) CD44−CD117− + PTX group (n=4); and iv) CD44−CD117− + PTX + CCL20 group (n=4). For xenografts, CD44+CD117+ (2×106) subsets or CD44−CD117− subsets cells (2×106) suspended in 0.5 ml PBS were subcutaneously injected into the left flank of each nude mouse to establish tumors. Then, PTX (10 mg/kg) and/or CCL20 (1 mg/kg) were injected intraperitoneally simultaneously on days 1, 3 and 7. Tumor size was monitored on the 3rd, 7th, 14th, 21st and 28th days (15). At the end of the experiment and under continuous anesthesia, the animals were euthanized by an overdose of pentobarbital (125 mg/kg). All experiments were approved by The Animal Care and Use Committee of the Shandong Provincial Hospital, Cheeloo College of Medicine, Shandong University.
Tumor sphere formation assay
In a 24-well plate, CD44+CD117+ or CD44−CD117− cells were harvested, counted and seeded with a low attachment (Corning, Inc.) at a density of 1.0×103 per well after isolation or treatment. Then, the cells were maintained in a DMEM/F12 medium (Sigma-Aldrich; Merck KGaA), without FBS, supplemented with 4 mg/ml heparin (Sigma-Aldrich; Merck KGaA), 20 ng/ml EGF (Sigma-Aldrich; Merck KGaA), 20 ng/ml bFGF (Sigma-Aldrich; Merck KGaA) and 2% B27 (Invitrogen; Thermo Fisher Scientific, Inc.). After 10–14 days, spheres were analyzed and images were captured with an inverted fluorescent microscope (Nikon Ni-U; Nikon Corporation; magnification, ×100).
Cytotoxicity determination
The IC50 of cells exposed to PTX was determined using a Cell Counting Kit (CCK)-8 assay (Dojindo Molecular Technologies, Inc.) according to the manufacturer's instructions. In brief, cells were inoculated at a concentration of 4×103/100 µl per well and cultured at 37°C for 12 h in a 96-well plate. The cells were then maintained at 37°C with 200 µl fresh medium containing different concentrations of PTX (0–50 nM) for 48 h. Following this, the cells were incubated with 20 µl CCK-8 solution at 37°C for 24 h. Then, the optical density value of each well was detected using a microplate reader at 450 nm (Thermo Fisher Scientific, Inc.).
Reactive oxygen species (ROS) detection
For ROS detection, 5×105 cells were seeded overnight in a 6-well plate and then treated with PTX (5 nM) at 37°C for 48 h. Subsequently, 5 µM carboxy 2′-7′-dichlorofluorescein diacetate was added in Hanks' Balanced Salt Solution and incubated with cells at room temperature for 0.5 h. Fresh tissues were harvested, followed by a single cell suspension preparation. Tissue was digested with trypsin to obtain single cell suspension. Cells were then inoculated in a 6-well plate as aforementioned. The levels of intracellular peroxide were measured using a flow cytometer (BD FACSAria; BD Biosciences) at 485 nm excitation and 520 nm emission to analyze the ROS generation.
Analysis of apoptosis
The apoptosis of cells was determined using flow cytometry and Hoechst assays. SKOV3 cells were seeded in a 6-well plate for flow cytometry and treated with PTX (5 nM) at 37°C for 48 h. The adherent and floating cells were harvested, washed with cold PBS and stained with FITC-conjugated Annexin V/PI (Beijing Solarbio Science & Technology Co., Ltd.), according to the manufacturer's instructions. The double-stained cells were then detected via Accuri C6 plus flow cytometry (BD Bioscience).
For Hoechst staining, 5×105 cells were seeded overnight in a 6-well plate and treated with PTX (5 nM) at 37°C for 48 h. Cells were then stained with 0.5 µg/ml Hoechst 33342 dye in a 5% CO2 incubator at 37°C for 25 min and were analyzed using a Nikon Ni-U fluorescence microscope (Nikon Corporation; magnification, ×100).
H&E staining
The tumors were removed and were fixed with 4% paraformaldehyde at 37°C for >24 h. Specimens were then dehydrated in isopropyl alcohol by xylene and embedded in paraffin. According to the manufacturer's instructions, specimens were cut into slices (thickness, 5 µm) and stained with H&E (Nanjing Jiancheng Bioengineering Institute) at 37°C for 24 h. For subsequent analysis, the slices were dehydrated and sealed with neutral resins. Images were captured with an inverted fluorescent microscope (Nikon Ni-U; Nikon Corporation; magnification, ×100). A total of 3 field of view were observed by microscopy.
TUNEL assay
The apoptosis of tumor tissue slices was assessed using the Clicl-iT Plus TUNEL assay (Thermo Fisher Scientific, Inc.). The tumors were removed and were fixed with 4% paraformaldehyde at 37°C for 24 h. According to the manufacturer's instructions, specimens were sectioned at 5 µm and stained with TUNEL reagent (Nanjing Jiancheng Bioengineering Institute) at 37°C for 1 h. After staining, the sections were sealed with a Prolong Gold antifade reagent with DAPI and images were captured with an inverted fluorescent microscope (Nikon Ni-U; Nikon Corporation; magnification, ×100). A total of 3 field of view were observed by microscopy.
Immunohistochemistry (IHC)
IHC was used to detect the expression level of CCR6 in xenograft mice. The tumors were removed and were fixed with 4% paraformaldehyde at 37°C for 48 h. To retrieve the antigen, 5-µm-thick slices from the center of paraffin-embedded tumor samples were collected, deparaffinized in xylene, rehydrated with gradient ethanol, immersed in a 10 mM citrate buffer (pH 6.0) and then heated in a microwave oven for 10 min. Subsequently, slices were incubated with 0.3% H2O2 at room temperature for 30 min to block the endogenous peroxidase activity and then with 1.5% normal goat serum at 37°C for 30 min (Takara Biotechnology Co., Ltd.) for blocking non-specific protein binding. Subsequently, the slices were immersed in the primary antibody in a humidity chamber at 4°C overnight. Slices were then incubated with a biotinylated goat anti-mouse IgG antibody (1:1,000; cat. no. AP124; Sigma-Aldrich; Merck KGaA) at 37°C for 30 min and visualized using the 3′3-diaminovbenzidine method. For further analysis, slices were then counterstained with hematoxylin for 30 min at 37°C and sealed with neutral resins. Images were captured with an inverted fluorescent microscope (Nikon Ni-U; Nikon Corporation; magnification, ×100).
Reverse transcription-quantitative (RT-q) PCR
TRIzol® reagent (Takara Biotechnology Co., Ltd.) was used to isolate total RNA samples from cells and tissues according to the manufacturer's instructions. A RT-qPCR kit (Takara Biotechnology Co., Ltd.) was used for cDNA synthesis, according to the manufacturer's instructions. With cDNA as a template, RT-qPCR was performed using SYBR Green (Takara Biotechnology Co., Ltd.) in an ABI 6500 system (Applied Biosciences; Thermo Fisher Scientific, Inc.) under the following conditions: Initial denaturation at 95°C for 90 sec, followed by 40 cycles of 95°C for 15 sec, 58°C for 21 sec and 72°C for 24 sec. The primers of genes are presented in Table I. With GAPDH as the internal control, the relative gene expression was calculated using the 2−ΔΔCq method (21).
Western blotting
The cells and tissue samples were lysed with a RIPA lysis buffer containing protease inhibitors (Sigma-Aldrich; Merck KGaA). The protein suspension was then collected and quantified using the BCA Protein Assay kit (Pierce; Thermo Fisher Scientific, Inc.). Then, 5 µg protein was boiled with a load buffer for 10 min, separated with 10% SDS-PAGE and transferred onto PVDF membranes (MilliporeSigma). Then, 5% skimmed milk was used to block the membrane at room temperature for 0.5 h, followed by primary antibody incubation at 4°C overnight. After washing with TBS −0.05% Tween-20 and polysorbate buffer, the membrane was incubated at room temperature with the secondary antibodies for 1 h. The secondary antibodies were horseradish peroxidase-labeled goat anti-rabbit IgG (1:5,000; cat. no. AP156P; Sigma-Aldrich; Merck KGaA) or goat anti-mouse IgG (1:5,000; cat. no. AP-308P; Sigma-Aldrich; Merck KGaA). The protein bands were visualized using an enhanced chemiluminescence kit (Beyotime Institute of Biotechnology) according to the manufacturer's protocol and quantified using the Gel-Pro-Analyzer 4.0 software (Media Cybernetics, Inc.). The primary antibodies were: CCR6 (1:1,000; cat. no. GTX71397; GeneTex, Inc.), ATP binding cassette subfamily G member 1 (ABCG1; cat. no. ab201776; 1:1,000; Abcam), Oct4 (1:1,000; cat. no. ab200834; Abcam), Notch1 (1:1,000; cat. no. 4380; Cell Signaling Technology, Inc.) and GAPDH (1:5,000; cat. no. SAB2103104; Sigma-Aldrich; Merck KGaA).
Statistical analysis
GraphPad Prism 7.0 (GraphPad Software, Inc.) was used for statistical analysis. All experiments were conducted in triplicate, and the data are presented as the mean ± SD. Comparisons between groups were conducted using paired Student's t-test, and multiple comparisons were made using a one-way ANOVA, followed by Tukey's test. P<0.05 was considered to indicate a statistically significant difference.
Results
CD44+CD117+ subgroup cells present higher stemness and PTX resistance than CD44−CD117−cells
The results demonstrated that the expression levels of CD44 and CD117 in the total subclones was significantly increased (P<0.05; Fig. S1). Once cells were isolated, CCR6 and Oct4 expression was determined using RT-qPCR. It was found that both Oct4 and CCR6 expression levels were significantly increased in CD44+CD117+ cells compared with CD44−CD117− cells (P<0.0001 and P<0.001; Fig. 1A). CD44+CD117+ cells also showed a higher sphere formation ability compared with CD44−CD117− subgroup cells, as detected in the sphere formation assay (Fig. 1B). Moreover, CD44+CD117+ subgroup cells exhibited significant higher protein expression levels of Oct4, CCR6, Notch1 and ABCG1 than CD44−CD117− cells (P<0.001; Fig. 1C and D).
Considering these findings, the PTX resistance of cells was determined using CCK-8 assay. The results indicated that the IC50 of CD44+CD117+ subgroup cells for PTX at 24 h (10.22 nM) and 48 h (5.04 nM) was higher compared with that in CD44−CD117− subgroups at 24 h (4.99 nM) and 48 h (2.51 nM), respectively (Fig. 1E). Moreover, the ROS level was lower in CD44+CD117 cells compared with CD44−CD117− cells after treatment with PTX (Fig. 2A and B). Furthermore, a lower apoptotic rate was observed in CD44+CD117+ subgroup cells compared with that in CD44−CD117− subgroup cells after treatment with PTX (P<0.01, Fig. 2C and D). The CCK-8 assay also indicated that CD44+CD117+ cells showed increased proliferation compared with CD44−CD117− cells after treatment with PTX (P<0.05 and P<0.001; Fig. 2E). These findings suggest that increased stemness and PTX resistance were observed in CD44+CD117+ subgroup cells than in CD44−CD117− cells, which could be attributed to the involvement of the Notch1 signaling pathway.
Silencing Notch1 reverses the effect of CCL20 on enhancing the stemness and PTX resistance of CD44+CD117+ cells
In order to investigate the underlying pathway involved in the regulation of stemness and PTX resistance, Notch1 was silenced in CD44+CD117+ cells that were treated with CCL20, a ligand of CCR6, followed by stemness and PTX resistance analyses. It was found that transfection of siNotch1 significantly inhibited the mRNA and protein expression levels of Notch1 (P<0.05, P<0.01 and P<0.001; Fig. S1).
The RT-qPCR analysis revealed that CCL20 treatment could significantly increase the expression level of Oct4, but silencing Notch1 could markedly reverse this upregulation in CD44+CD117+ cells (P<0.001; Fig. 3A). Moreover, via western blot analysis, it was identified that the protein expression levels of Oct4, ABCG1 and CCR6 in CD44+CD117+ cells were significantly be increased by CCL20, but that siNotch1 could markedly reverse the effect of CCL20 in elevating Oct4, ABCG1 and CCR6 expression in CD44+CD117+ cells (P<0.001 and P<0.001; Fig. 3B and C). Sphere formation assays also demonstrated that CCL20 could increase the sphere formation capacity of CD44+CD117+ cells, while siNotch1 could notably impair the promotive effect of CCL20 (Fig. 3D). Thus, it was suggested that the CCL20/CCR6 axis may increase the stemness of CD44+CD117+ cells via the Notch1 pathway.
Next, the PTX resistance of CD44+CD117+ cells after transfection with siNotch1 and CCL20 treatment was determined. The results demonstrated that CCL20 could markedly increase IC50 of CD44+CD117+ cells (IC50=17.51 nM, 8.41 nM) to PTX compared with the control group (IC50=10.98 nM, 4.86 nM) at 24 and 48 h, while siNotch1 could significantly reverse the effect of CCL20 on PTX resistance of CD44+CD117+ at 24 h (IC50=7.92 nM, 4.22 nM; Fig. 4A), respectively. After further analysis, it was observed that CCL20 could also markedly ameliorate the ROS level in CD44+CD117+ cells induced by PTX, while siNotch1 could attenuate the effect of CCL20 in alleviating ROS levels in CD44+CD117+ cells after treatment with PTX (Fig. 4B). Moreover, the apoptosis of CD44+CD117+ cells was significantly decreased by CCL20 after treatment with PTX (P<0.0001), but siNotch1 could partially reverse the effect of CCL20 in suppressing apoptosis of CD44+CD117+ cells induced by PTX (P<0.001; Fig. 4C-E). In addition, the CCK-8 assays identified that CCL20 could increase the proliferation of CD44+CD117+ cells, and the promotive effect of CCL20 after treatment with PTX could be impaired by siNotch1 (Fig. 4F). This evidence suggested that the CCL20/CCR6 axis nay increase the stemness and PTX resistance of CD44+CD117+ cells via the Notch1 signaling pathway.
A CCL20/CCR6 axis promotes tumorigenicity and PTX resistance of CD44+CD117+ cells in ovarian cancer via the Notch1 signaling pathway
To confirm the role of the CCL20/CCR6 axis in PTX resistance, SKOV3 CD44+CD117+ and CD44−CD117− subgroup cells were subjected to the xenograft assay, followed by CCL20 and/or PTX treatment. The results demonstrated that CCL20 could significantly increase the tumor volume of CD44+CD117+ and CD44−CD117− cells in nude mice compared with the respective PTX treatment alone groups (Fig. 5A and B). From the tumor growth curve analysis, it was found that tumors formed by CD44+CD117+ cells grew more quickly than those formed by CD44−CD117− (P<0.001; Fig. 5B). The growth of all tumors could be significantly increased by treating with PTX, but the volume of tumor-derived from CD44+CD117+ cells was significantly higher compared with that from CD44−CD117− cells (P<0.01; Fig. 5B). Moreover, the weight of tumors derived from CD44+CD117+ cells was significantly greater than that derived from CD44−CD117− cells treated with PTX (P<0.0001; Fig. 5C). The tumor weight was significantly increased by CCL20 treatment in CD44+CD117+ and CD44−CD117− cells treated with PTX. However, tumor weight in the CD44+CD117+ group was significantly higher than that in the CD44−CD117− group (P<0.001; Fig. 5C).
H&E staining analysis tumors of the CD44+CD117+ groups revealed poorly differentiated morphology compared with the CD44−CD117− groups, as characterized by the number of small, round cells with hyperchromatic nuclei and scanty cytoplasm, after treatment with PTX. CCL20 treatment could notably decrease tumor differentiation in the CD44+CD117+ group compared with in the CD44−CD117− group (Fig. 5D). A higher expression level of CCR6 in the tumors was identified in the CD44+CD117+ group compared with the CD44−CD117− group via the IHC analysis. Furthermore, CCL20 treatment could markedly increase the expression level of CCR6 in tumors of the CD44+CD117+ group compared with in the CD44−CD117− group (Fig. 5E).
As observed in further investigations, the CD44+CD117+ group tissue presented with higher proliferation but lower apoptosis compared with the tumor tissue of the CD44−CD117− group after treatment with PTX (Fig. 6A and B). In the CD44+CD117+ and CD44−CD117− groups, CCL20 treatment could significantly accelerate proliferation but inhibit apoptosis after treatment with PTX. However, these changes in the CD44+CD117+ group were higher compared with those in the CD44−CD117− group (Fig. 6A and B). Furthermore, ROS levels of tumors in the CD44+CD117+ group were markedly lower compared with those in the CD44−CD117− group after treatment with PTX or CCL20 (Fig. 6C). The stemness and involved signaling pathway were also determined in tumor tissue to further confirm the underlying mechanism. The results indicated that the expression levels of Oct4, CCR6, ABCG1 and Notch1 in tumor of CD44+CD117+ and CD44−CD117− groups could be significantly accelerated by CCL20 treatment, while such facilitation was increased in the CD44+CD117+ group compared with the CD44−CD117− group (P<0.0001; Fig. 6D and E). Taken together, these findings suggested that CCL20/CCR6/Notch1 signaling could markedly facilitate the stemness and proliferation of the CD44+CD117+ subgroup cancer cells to promote ovarian cancer resistance to PTX.
Discussion
Cancer stem-like cells are widely accepted to be responsible for drug resistance of ovarian cancer (21). Therefore, it is of great significance to reveal the role of cancer stem-like cells in drug resistance. It has been shown that two surface markers of cancer cells in ovarian cancer are CD44 and CD117 (22). In the present study, CD44+CD117+ subgroup cells were isolated from SKOV3 cells and used for PTX resistance investigation.
Several studies have reported that CD44 and CD117 are two stem cell markers in ovarian cancer, and that CD44+CD117+ cancer cells present with a powerful survival ability and tumorigenic capability (23,24). In ovarian cancer cells, the overexpression of miR-199a could significantly inhibit CD44 expression and attenuate multidrug resistance and tumorigenicity (25). Moreover, a clinical meta-analysis revealed that a marker for the poor prognosis of ovarian cancer could be the upregulation of CD117 (26). In the present study, CD44+CD117+ cells were isolated from SKOV3 cells. IC50 analysis identified that CD44+CD117+ cells had a higher IC50 concentration of PTX compared with CD44−CD117− cells, suggesting that CD44+CD117+ cells showed a higher PTX resistance. In addition, the expression levels of other cancer stem-like cell markers, including Oct4 and ABCG1, were higher compared with those in the CD44−CD117− cells, indicating that CD44+CD117+ cells had a more powerful stemness ability than CD44−CD117− cells. After treatment with PTX in both in vitro and in vivo, CD44+CD117+ cells also presented higher sphere formation proliferation abilities, and lower apoptosis and ROS levels. It is well known that oxidative stress is directly assessed by measuring ROS. Moreover, ROS are involved in the regulation of cell inventory and death (27). ROS is also reported to be closely associated with inflammation, aging and chronic diseases, including cancer (28). Collectively, the present results suggested that CD44+CD117+ cells presented a powerful stemness and tumorigenic capability than CD44−CD117− cells.
The only known chemokine ligand for CCR6 is CCL20, and this axis has been reported to serve a critical role in the development of numerous types of cancer, such as lung (29), colorectal (30) and thyroid cancers (31). In a recent study, cisplatin was shown to stimulate macrophages to secrete CCL20 and promote ovarian cancer cell migration via the CCL20/CCR6 axis (16). CCL20 can also enhance the chemotherapy resistance of ovarian cancer by regulating ABCB1 expression (31). In the present study, the expression levels of CCL20 and CCR6 were significantly upregulated in CD44+CD117+ cells compared with those in CD44−CD117− cells. Moreover, CCL20 treatment could significantly increase the PTX resistance, proliferation and tumorigenic abilities of CD44+CD117+ cells, but decreased apoptosis and ROS in vitro and in vivo.
Accumulating evidence has shown that the CCL20/CCR6 axis also served a critical role in regulating cancer stem cells (15,32–34). However, there are relatively limited associated reports on ovarian cancer. In the current study, it was found that CCL20 treatment could significantly increase the expression levels of stem cell markers, including Oct4, and ABCG1. Previous studies have also suggested that the CCL20/CCR6 axis may play a key role in regulating ovarian cancer stem cells (16,35). Collectively, these findings suggested that the CCL20/CCR6 axis may act as a promotor in maintaining stemness and drug resistance of ovarian cancer. As a ligand of CCR6, CCL20 promotes ovarian stem cells and PTX resistance of ovarian stem cell-like cancer cells. However, whether PTX resistance cancels the silencing of CCR6 needs to be further examined.
A common dysregulated pathway in the development of cancer is the Notch1 signaling pathway, which has been shown to play a critical role in regulating stemness, proliferation, metastasis and drug resistance of cancer (36–38). As shown in a previous study, the hypoxia-induced Notch/SOX2 axis was crucial for maintaining cancer stem-like cells in ovarian cancer (39). It has also been reported that galectin-3 could activate the intracellular domain of Notch1 to maintain the stemness of ovarian cancer stem cells (40). In the present study, it was identified that siNotch1 could significantly decrease the expression levels of Oct4, ABCG1 and CCR6 in CD44+CD117+ cells treated with PTX. Furthermore, the effects of CCL20 could be significantly reversed by siNotch1 to enhance drug resistance and tumorigenic capability, as well as reduce the apoptosis and ROS levels of CD44+CD117+ cells treated with PTX in vivo and in vitro. Such findings also suggested that the CCL20/CCR6 axis may promote the stemness and tumorigenicity of CD44+CD117+ cells by activating the Notch1 signaling pathway. However, the lack of research on the sensitivity of CD44+CD117+ cells to platinum may be considered a potential limitation of this study.
In conclusion, the present study demonstrated that the CD44+CD117+ subgroup SKOV3 cells showed increased stemness and drug resistance compared with CD44−CD117− cells. The results indicated that the stemness, tumorigenicity and PTX resistance of CD44+CD117+ cells could significantly enhance CCL20 by activating the Notch1 signaling pathway.
Supplementary Material
Supporting Data
Acknowledgements
Not applicable.
Funding
This study was supported by the Natural Science Foundation of Shandong Province (grant no. 2015GSF118140).
Availability of data and materials
The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.
Authors' contribution
MC, YT and LZ conceived and designed the research. MC, JS, CF and YL performed the experiments. MC, YT and LZ confirm the authenticity of all the raw data. JS, CF and YL analyzed the data and prepared the figures. MC and JS edited and revised the manuscript. All authors approved the final version of the manuscript.
Ethics approval and consent to participate
The Animal Care and Use Committee of the Shandong Provincial Hospital, Cheeloo College of Medicine, Shandong University, authorized the present study. All methods were carried out in accordance with relevant guidelines and regulations. The animal study was carried out in compliance with the ARRIVE guidelines.
Patient consent for publication
Not applicable.
Competing interests
The authors declare that they have no competing interests.
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