Chemokine CCL20 promotes the paclitaxel resistance of CD44<sup>+</sup>CD117<sup>+</sup> cells via the Notch1 signaling pathway in ovarian cancer

Chen,Min; Su,Juan; Feng,Chunmei; Liu,Ying; Zhao,Li; Tian,Yongjie

doi:10.3892/mmr.2021.12274

September-2021 Volume 24 Issue 3

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September-2021 Volume 24 Issue 3

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Article Open Access

Chemokine CCL20 promotes the paclitaxel resistance of CD44⁺CD117⁺ cells via the Notch1 signaling pathway in ovarian cancer

Authors:
- Min Chen
- Juan Su
- Chunmei Feng
- Ying Liu
- Li Zhao
- Yongjie Tian
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Affiliations: Department of Obstetrics and Gynecology, Shandong Provincial Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong 250021, P.R. China, Department of Obstetrics and Gynecology, Taian City Central Hospital, Taian, Shandong 271000, P.R. China

Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Article Number: 635
|
Published online on: July 6, 2021

https://doi.org/10.3892/mmr.2021.12274
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Abstract

Studies have found that C‑C motif chemokine ligand 20 (CCL20)/C‑C motif chemokine receptor 6 (CCR6)/notch receptor 1 (Notch1) signaling serves an important role in various diseases, but its role and mechanism in ovarian cancer remains to be elucidated. The aim of the present study was to investigate the underlying mechanism of CCL20/CCR6/Notch1 signaling in paclitaxel (PTX) resistance of a CD44⁺CD117⁺ subgroup of cells in ovarian cancer. The CD44⁺CD117⁺ cells were isolated from SKOV3 cells, followed by determination of the PTX resistance and the CCR6/Notch1 axis. Notch1 was silenced in the CD44⁺CD117⁺ subgroup and these cells were treated with CCL20, followed by examination of PTX resistance and the CCR6/Notch1 axis. Furthermore, in nude mice, CD44⁺CD117⁺ and CD44^‑CD117^‑ cells were used to establish the xenograft model and cells were treated with PTX and/or CCL20, followed by proliferation, apoptosis, reactive oxygen species (ROS) and mechanism analyses. Higher expression levels of Oct4, CCR6, Notch1 and ATP binding cassette subfamily G member 1 (ABCG1), increased sphere formation ability, IC₅₀ and proliferative ability, as well as lower ROS levels and apoptosis were observed in CD44⁺CD117⁺ cells compared with the CD44^‑CD117^‑ cells. It was found that CCL20 could significantly increase the expression levels of Oct4, CCR6, Notch1 and ABCG1, enhance the IC₅₀, sphere formation ability and proliferation, as well as decrease the ROS and apoptosis levels in the CD44⁺CD117⁺ cells. However, Notch1 knockdown could markedly reverse these changes. Moreover, CCL20 could significantly increase the proliferation and expression levels of Oct4, CCR6, Notch1 and ABCG1 in the CD44⁺CD117⁺ groups compared with the CD44^‑CD117^‑ groups. After treatment with PTX, apoptosis and ROS levels were decreased in the CD44⁺CD117⁺ groups compared with the CD44^‑CD117^‑ groups. Collectively, the present results demonstrated that, via the Notch1 pathway, CCL20/CCR6 may promote the stemness and PTX resistance of CD44⁺CD117⁺ cells in ovarian cancer.

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