Open Access

Interactions between the ERK1/2 signaling pathway and PCAF play a key role in PE‑induced cardiomyocyte hypertrophy

  • Authors:
    • Qian Mao
    • Shuqi Wu
    • Chang Peng
    • Bohui Peng
    • Xiaomei Luo
    • Lixin Huang
    • Huanting Zhang
  • View Affiliations

  • Published online on: July 6, 2021     https://doi.org/10.3892/mmr.2021.12275
  • Article Number: 636
  • Copyright: © Mao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Cardiomyocyte hypertrophy is a compensatory phase of chronic heart failure that is induced by the activation of multiple signaling pathways. The extracellular signal‑regulated protein kinase (ERK) signaling pathway is an important regulator of cardiomyocyte hypertrophy. In our previous study, it was demonstrated that phenylephrine (PE)‑induced cardiomyocyte hypertrophy involves the hyperacetylation of histone H3K9ac by P300/CBP‑associated factor (PCAF). However, the upstream signaling pathway has yet to be fully identified. In the present study, the role of the extracellular signal‑regulated protein kinase (ERK)1/2 signaling pathway in PE‑induced cardiomyocyte hypertrophy was investigated. The mice cardiomyocyte hypertrophy model was successfully established by treating cells with PE in vitro. The results showed that phospho‑(p‑)ERK1/2 interacted with PCAF and modified the pattern of histone H3K9ac acetylation. An ERK inhibitor (U0126) and/or a histone acetylase inhibitor (anacardic acid; AA) attenuated the overexpression of phospho‑ERK1/2 and H3K9ac hyperacetylation by inhibiting the expression of PCAF in PE‑induced cardiomyocyte hypertrophy. Moreover, U0126 and/or AA could attenuate the overexpression of several biomarker genes related to cardiac hypertrophy (myocyte enhancer factor 2C, atrial natriuretic peptide, brain natriuretic peptide and β‑myosin heavy chain) and prevented cardiomyocyte hypertrophy. These results revealed a novel mechanism in that AA protects against PE‑induced cardiomyocyte hypertrophy in mice via the ERK1/2 signaling pathway, and by modifying the acetylation of H3K9ac. These findings may assist in the development of novel methods for preventing and treating hypertrophic cardiomyopathy.
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September-2021
Volume 24 Issue 3

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Mao Q, Wu S, Peng C, Peng B, Luo X, Huang L and Zhang H: Interactions between the ERK1/2 signaling pathway and PCAF play a key role in PE‑induced cardiomyocyte hypertrophy. Mol Med Rep 24: 636, 2021
APA
Mao, Q., Wu, S., Peng, C., Peng, B., Luo, X., Huang, L., & Zhang, H. (2021). Interactions between the ERK1/2 signaling pathway and PCAF play a key role in PE‑induced cardiomyocyte hypertrophy. Molecular Medicine Reports, 24, 636. https://doi.org/10.3892/mmr.2021.12275
MLA
Mao, Q., Wu, S., Peng, C., Peng, B., Luo, X., Huang, L., Zhang, H."Interactions between the ERK1/2 signaling pathway and PCAF play a key role in PE‑induced cardiomyocyte hypertrophy". Molecular Medicine Reports 24.3 (2021): 636.
Chicago
Mao, Q., Wu, S., Peng, C., Peng, B., Luo, X., Huang, L., Zhang, H."Interactions between the ERK1/2 signaling pathway and PCAF play a key role in PE‑induced cardiomyocyte hypertrophy". Molecular Medicine Reports 24, no. 3 (2021): 636. https://doi.org/10.3892/mmr.2021.12275