Open Access

miR‑125a‑5p and miR‑7 inhibits the proliferation, migration and invasion of vascular smooth muscle cell by targeting EGFR

  • Authors:
    • Hualan Zhou
    • Sen Lin
    • Youdong Hu
    • Dianxuan Guo
    • Yun Wang
    • Xia Li
  • View Affiliations

  • Published online on: August 9, 2021     https://doi.org/10.3892/mmr.2021.12347
  • Article Number: 708
  • Copyright: © Zhou et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The ectopic proliferation, migration and invasion of vascular smooth muscle cells (VSMCs) contributes to the progression of various human vascular diseases. Accumulating evidence has demonstrated that microRNAs (miRs) exert vital functions in the proliferation and invasion of VSMCs. The current study aimed to elucidate the functions of miR‑125a‑5p and miR‑7 in VSMCs and investigate the associated molecular mechanisms. The results of EdU and reverse transcription‑quantitative PCR assays revealed that platelet‑derived growth factor (PDGF)‑BB enhanced the proliferation of VSMCs and significantly reduced the expression of miR‑125a‑5p and miR‑7. miR‑125a‑5p or miR‑7 overexpression significantly ameliorated PDGF‑BB‑induced proliferation, migration and invasion of VSMCs. Furthermore, the results demonstrated that epidermal growth factor receptor (EGFR) may be a target mRNA of miR‑125a‑5p and miR‑7 in VSMCs. The results of western blot analysis indicated that co‑transfection of miR‑125a‑5p mimics or miR‑7 mimics distinctly decreased the protein expression of EGFR in EGFR‑overexpressed VSMCs. Moreover, rescue experiments indicated that EGFR overexpression alleviated the suppressive impact of the miR‑125a‑5p and miR‑7 s on the growth, migration and invasion of VSMCs. In conclusion, the current study identified that miR‑125a‑5p and miR‑7 repressed the growth, migration and invasion of PDGF‑BB‑stimulated VSMCs by, at least partially, targeting EGFR. The current study verified that miR‑125a‑5p and miR‑7 may be used as feasible therapeutic targets for cardiovascular diseases.
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October-2021
Volume 24 Issue 4

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Zhou H, Lin S, Hu Y, Guo D, Wang Y and Li X: miR‑125a‑5p and miR‑7 inhibits the proliferation, migration and invasion of vascular smooth muscle cell by targeting EGFR. Mol Med Rep 24: 708, 2021
APA
Zhou, H., Lin, S., Hu, Y., Guo, D., Wang, Y., & Li, X. (2021). miR‑125a‑5p and miR‑7 inhibits the proliferation, migration and invasion of vascular smooth muscle cell by targeting EGFR. Molecular Medicine Reports, 24, 708. https://doi.org/10.3892/mmr.2021.12347
MLA
Zhou, H., Lin, S., Hu, Y., Guo, D., Wang, Y., Li, X."miR‑125a‑5p and miR‑7 inhibits the proliferation, migration and invasion of vascular smooth muscle cell by targeting EGFR". Molecular Medicine Reports 24.4 (2021): 708.
Chicago
Zhou, H., Lin, S., Hu, Y., Guo, D., Wang, Y., Li, X."miR‑125a‑5p and miR‑7 inhibits the proliferation, migration and invasion of vascular smooth muscle cell by targeting EGFR". Molecular Medicine Reports 24, no. 4 (2021): 708. https://doi.org/10.3892/mmr.2021.12347