Assessing the protective effects of cryptotanshinone on CoCl<sub>2</sub>‑induced hypoxia in RPE cells

Gu,Yu; Liu,Weiming; Liu,Gaoqin; Li,Xin; Lu,Peirong

doi:10.3892/mmr.2021.12379

October-2021 Volume 24 Issue 4

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October-2021 Volume 24 Issue 4

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Article Open Access

Assessing the protective effects of cryptotanshinone on CoCl₂‑induced hypoxia in RPE cells

Authors:
- Yu Gu
- Weiming Liu
- Gaoqin Liu
- Xin Li
- Peirong Lu
View Affiliations / Copyright

Affiliations: Department of Ophthalmology, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, P.R. China

Copyright: © Gu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Article Number: 739
|
Published online on: August 20, 2021

https://doi.org/10.3892/mmr.2021.12379
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Abstract

The development of several retinal diseases is closely related to hypoxia. As a component of the Traditional Chinese medicine Salvia miltiorrhiza, the effects of cryptotanshinone (CT) on retinal cells under hypoxic conditions are not well understood. The aim of the present study was to explore how CT exerted its protective effects on retinal pigment epithelium (RPE) cells under hypoxic conditions induced by cobalt chloride (CoCl₂). The effects of CT were investigated using a Cell Counting Kit‑8 assay, Annexin V‑FITC/PI staining, reverse transcription‑quantitative PCR and western blotting in ARPE‑19 cells. CT (10 and 20 µM) reduced the CoCl₂‑induced increase in vascular endothelial growth factor expression and hypoxia‑inducible transcription factor‑1α expression in ARPE‑19 cells. Additionally, CT alleviated hypoxia‑induced apoptosis by regulating Bcl‑2 and Bax protein expression. CT treatment also reduced the increase in the mRNA levels of IL‑6, IL‑1β and TNF‑α induced by CoCl₂. In summary, CT may protect RPE cells against apoptosis and inflammation in CoCl₂‑induced hypoxia, and these results warrant further in vivo study into its value as a drug for treating hypoxic eye diseases.

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