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Silencing the lncRNA NORAD inhibits EMT of head and neck squamous cell carcinoma stem cells via miR‑26a‑5p

  • Authors:
    • Weiming Hu
    • Yong Zhao
    • Lizhong Su
    • Zuliang Wu
    • Wenjing Jiang
    • Xiaoze Jiang
    • Ming Liu
  • View Affiliations / Copyright

    Affiliations: Department of Otorhinolaryngology, Zhejiang Provincial People's Hospital, People's Hospital of Hangzhou Medical College, Hangzhou, Zhejiang 310014, P.R. China, Department of Otorhinolaryngology, XIXI Hospital of Hangzhou, Hangzhou, Zhejiang 310012, P.R. China, Department of Otorhinolaryngology, Zhejiang Hospital, Hangzhou, Zhejiang 310012, P.R. China
    Copyright: © Hu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 743
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    Published online on: August 25, 2021
       https://doi.org/10.3892/mmr.2021.12383
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Abstract

Cancer stem cells are closely associated with tumor metastasis or recurrence. According to previous literature reports, microRNA (miR)‑26a has an inhibitory effect on head and neck squamous cell carcinoma (HNSCC), and the long non‑coding RNA (lncRNA) non‑coding RNA activated by DNA damage (NORAD) has been found to interact with miR‑26a‑5p. The present study aimed to investigate the regulation and mechanism of NORAD and miR‑26a‑5p in the epithelial‑mesenchymal transition (EMT) of HNSCC stem cells. An ALDEFLUOR stem cell detection kit, a flow cytometer, a self‑renewal ability test and western blotting were used to sort and identify HNSCC stem cells. The ENCORI website and a dual‑luciferase assay were used to assess the relationship between genes. The mRNA and protein expression levels of NORAD, miR‑26a‑5p and EMT‑related genes were detected via reverse transcription‑quantitative PCR and western blotting. Functional experiments (MTT assay, flow cytometry, wound healing assay and Transwell assay) were conducted to analyze the effects of NORAD and miR‑26a‑5p on HNSCC stem cells. The successfully sorted aldehyde dehydrogenase (ALDH)+ cells had a self‑renewal capacity and displayed upregulated expression levels of CD44, Oct‑4 and Nanog. NORAD knockdown, achieved using small interfering (si)RNA, downregulated the expression levels of tumor markers in ALDH+ cells. siNORAD inhibited cell vitality, migration and invasion, as well as promoted apoptosis, increased the expression of epithelial cell markers and decreased the expression of interstitial cell markers in HNSCC stem cells. miR‑26a‑5p was a downstream gene of NORAD, and knockdown of miR‑26a‑5p partially offset the regulatory effect of siNORAD on HNSCC stem cells. Collectively, the present study demonstrated that NORAD knockdown attenuated the migration, invasion and EMT of HNSCC stem cells via miR‑26a‑5p.
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Copy and paste a formatted citation
Spandidos Publications style
Hu W, Zhao Y, Su L, Wu Z, Jiang W, Jiang X and Liu M: Silencing the lncRNA NORAD inhibits EMT of head and neck squamous cell carcinoma stem cells via miR‑26a‑5p. Mol Med Rep 24: 743, 2021.
APA
Hu, W., Zhao, Y., Su, L., Wu, Z., Jiang, W., Jiang, X., & Liu, M. (2021). Silencing the lncRNA NORAD inhibits EMT of head and neck squamous cell carcinoma stem cells via miR‑26a‑5p. Molecular Medicine Reports, 24, 743. https://doi.org/10.3892/mmr.2021.12383
MLA
Hu, W., Zhao, Y., Su, L., Wu, Z., Jiang, W., Jiang, X., Liu, M."Silencing the lncRNA NORAD inhibits EMT of head and neck squamous cell carcinoma stem cells via miR‑26a‑5p". Molecular Medicine Reports 24.5 (2021): 743.
Chicago
Hu, W., Zhao, Y., Su, L., Wu, Z., Jiang, W., Jiang, X., Liu, M."Silencing the lncRNA NORAD inhibits EMT of head and neck squamous cell carcinoma stem cells via miR‑26a‑5p". Molecular Medicine Reports 24, no. 5 (2021): 743. https://doi.org/10.3892/mmr.2021.12383
Copy and paste a formatted citation
x
Spandidos Publications style
Hu W, Zhao Y, Su L, Wu Z, Jiang W, Jiang X and Liu M: Silencing the lncRNA NORAD inhibits EMT of head and neck squamous cell carcinoma stem cells via miR‑26a‑5p. Mol Med Rep 24: 743, 2021.
APA
Hu, W., Zhao, Y., Su, L., Wu, Z., Jiang, W., Jiang, X., & Liu, M. (2021). Silencing the lncRNA NORAD inhibits EMT of head and neck squamous cell carcinoma stem cells via miR‑26a‑5p. Molecular Medicine Reports, 24, 743. https://doi.org/10.3892/mmr.2021.12383
MLA
Hu, W., Zhao, Y., Su, L., Wu, Z., Jiang, W., Jiang, X., Liu, M."Silencing the lncRNA NORAD inhibits EMT of head and neck squamous cell carcinoma stem cells via miR‑26a‑5p". Molecular Medicine Reports 24.5 (2021): 743.
Chicago
Hu, W., Zhao, Y., Su, L., Wu, Z., Jiang, W., Jiang, X., Liu, M."Silencing the lncRNA NORAD inhibits EMT of head and neck squamous cell carcinoma stem cells via miR‑26a‑5p". Molecular Medicine Reports 24, no. 5 (2021): 743. https://doi.org/10.3892/mmr.2021.12383
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