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Atrial natriuretic peptide protects vertebral endplate chondrocytes against H2O2‑induced apoptosis and oxidative stress through activation of the Nrf2/HO‑1 signaling pathway

  • Authors:
    • Feiping He
    • Jingying Gai
    • Jun Wang
    • Lei Tang
    • Yifeng Liu
    • Qingchun Feng
  • View Affiliations / Copyright

    Affiliations: Department of Spinal Surgery, The Second People's Hospital of Hunan Province, Brain Hospital of Hunan Province, Changsha, Hunan 410007, P.R. China, Department of Cardiovascular Medicine, The Second People's Hospital of Hunan Province, Brain Hospital of Hunan Province, Changsha, Hunan 410007, P.R. China
    Copyright: © He et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 754
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    Published online on: September 1, 2021
       https://doi.org/10.3892/mmr.2021.12394
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Abstract

The present study aimed to investigate the effect of atrial natriuretic peptide (ANP) on cell apoptosis and oxidative stress in H2O2‑induced vertebral endplate chondrocytes (EPCs), and to assess the associated mechanisms involved. Cell viability and apoptosis were evaluated using the Cell Counting Kit‑8 method and TUNEL assay, respectively. In addition, the scavenging capability was detected using various enzymatic assays, and the quantity of nitric oxide (NO) and malondialdehyde (MDA), and activity of superoxide dismutase (SOD) were assessed. The expression levels of apoptosis‑related proteins, activation of the nuclear factor erythroid 2‑related factor 2 (Nrf2)/heme oxygenase‑1 (HO‑1) signaling pathway induced by H2O2 and the effect of treatment with ANP on vertebral EPCs were detected by western blotting. The results revealed that ANP protected EPCs from H2O2‑induced cell damage. H2O2‑induced intracellular MDA was decreased by ANP, and the levels of SOD and NO were increased in the presence of ANP. ANP also inhibited the H2O2‑induced alterations in the expression levels of cleaved‑caspase‑3, Bax and Bcl‑2. Finally, ANP blocked H2O2‑induced oxidative stress through activating the Nrf2/HO‑1 signaling pathway. These findings suggested that ANP may effectively protect EPCs through inhibition of H2O2‑induced oxidant injury and cell death by activating the Nrf2/HO‑1 signaling pathway.
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Copy and paste a formatted citation
Spandidos Publications style
He F, Gai J, Wang J, Tang L, Liu Y and Feng Q: Atrial natriuretic peptide protects vertebral endplate chondrocytes against H<sub>2</sub>O<sub>2</sub>‑induced apoptosis and oxidative stress through activation of the Nrf2/HO‑1 signaling pathway. Mol Med Rep 24: 754, 2021.
APA
He, F., Gai, J., Wang, J., Tang, L., Liu, Y., & Feng, Q. (2021). Atrial natriuretic peptide protects vertebral endplate chondrocytes against H<sub>2</sub>O<sub>2</sub>‑induced apoptosis and oxidative stress through activation of the Nrf2/HO‑1 signaling pathway. Molecular Medicine Reports, 24, 754. https://doi.org/10.3892/mmr.2021.12394
MLA
He, F., Gai, J., Wang, J., Tang, L., Liu, Y., Feng, Q."Atrial natriuretic peptide protects vertebral endplate chondrocytes against H<sub>2</sub>O<sub>2</sub>‑induced apoptosis and oxidative stress through activation of the Nrf2/HO‑1 signaling pathway". Molecular Medicine Reports 24.5 (2021): 754.
Chicago
He, F., Gai, J., Wang, J., Tang, L., Liu, Y., Feng, Q."Atrial natriuretic peptide protects vertebral endplate chondrocytes against H<sub>2</sub>O<sub>2</sub>‑induced apoptosis and oxidative stress through activation of the Nrf2/HO‑1 signaling pathway". Molecular Medicine Reports 24, no. 5 (2021): 754. https://doi.org/10.3892/mmr.2021.12394
Copy and paste a formatted citation
x
Spandidos Publications style
He F, Gai J, Wang J, Tang L, Liu Y and Feng Q: Atrial natriuretic peptide protects vertebral endplate chondrocytes against H<sub>2</sub>O<sub>2</sub>‑induced apoptosis and oxidative stress through activation of the Nrf2/HO‑1 signaling pathway. Mol Med Rep 24: 754, 2021.
APA
He, F., Gai, J., Wang, J., Tang, L., Liu, Y., & Feng, Q. (2021). Atrial natriuretic peptide protects vertebral endplate chondrocytes against H<sub>2</sub>O<sub>2</sub>‑induced apoptosis and oxidative stress through activation of the Nrf2/HO‑1 signaling pathway. Molecular Medicine Reports, 24, 754. https://doi.org/10.3892/mmr.2021.12394
MLA
He, F., Gai, J., Wang, J., Tang, L., Liu, Y., Feng, Q."Atrial natriuretic peptide protects vertebral endplate chondrocytes against H<sub>2</sub>O<sub>2</sub>‑induced apoptosis and oxidative stress through activation of the Nrf2/HO‑1 signaling pathway". Molecular Medicine Reports 24.5 (2021): 754.
Chicago
He, F., Gai, J., Wang, J., Tang, L., Liu, Y., Feng, Q."Atrial natriuretic peptide protects vertebral endplate chondrocytes against H<sub>2</sub>O<sub>2</sub>‑induced apoptosis and oxidative stress through activation of the Nrf2/HO‑1 signaling pathway". Molecular Medicine Reports 24, no. 5 (2021): 754. https://doi.org/10.3892/mmr.2021.12394
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