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Salvianolic acid B improves autophagic dysfunction and decreases the apoptosis of cholesterol crystal‑induced macrophages via inhibiting the Akt/mTOR signaling pathway

  • Authors:
    • Mengqi Sun
    • Yun Ye
    • Yilan Huang
    • Wenxian Yin
    • Zhaolan Yu
    • Shurong Wang
  • View Affiliations / Copyright

    Affiliations: Drug Clinical Trial Institution, The Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan 646000, P.R. China, Department of Pharmacy, The Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan 646000, P.R. China, Department of Pharmacy, The Affiliated Hospital of Traditional Chinese Medicine Southwest Medical University, Luzhou, Sichuan 646000, P.R. China, Department of Nephrology, The Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan 646000, P.R. China
    Copyright: © Sun et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 763
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    Published online on: September 3, 2021
       https://doi.org/10.3892/mmr.2021.12403
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Abstract

Progressive macrophage dysfunction and apoptosis are some of the major events that occur during atherogenesis. To further investigate the intrinsic association between atherosclerosis (AS) and macrophage apoptosis and autophagy, cholesterol crystals (CHCs) were used to stimulate RAW264.7 macrophages to establish a macrophage model of advanced AS. Cells in the CHC group were treated with salvianolic acid B (Sal B) to evaluate its protective effects and reveal its underlying molecular mechanism. The results demonstrated that treatments with Sal B significantly improved autophagy dysfunction and reduced the apoptotic rate of CHC‑induced macrophages. Furthermore, Sal B significantly attenuated CHC‑induced release of proinflammatory factors (TNF‑α and IL‑6) by macrophages. Treatment of macrophages with a specific inhibitor of autophagy (3‑methyladenine) significantly reversed Sal B‑mediated effects on autophagy, suggesting that Sal B‑induced autophagy may display a protective effect in CHC‑induced macrophages. Furthermore, pretreatment of CHC‑induced macrophages with insulin significantly decreased Sal B‑induced autophagy, indicating that the Akt/mTOR signaling pathway may serve as a critical mediator in regulating Sal B‑mediated cell death. Taken together, the present study demonstrated that Sal B improved autophagic dysfunction and reduced the apoptosis of CHC‑induced macrophages via inhibiting the Akt/mTOR signaling pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Sun M, Ye Y, Huang Y, Yin W, Yu Z and Wang S: Salvianolic acid B improves autophagic dysfunction and decreases the apoptosis of cholesterol crystal‑induced macrophages via inhibiting the Akt/mTOR signaling pathway. Mol Med Rep 24: 763, 2021.
APA
Sun, M., Ye, Y., Huang, Y., Yin, W., Yu, Z., & Wang, S. (2021). Salvianolic acid B improves autophagic dysfunction and decreases the apoptosis of cholesterol crystal‑induced macrophages via inhibiting the Akt/mTOR signaling pathway. Molecular Medicine Reports, 24, 763. https://doi.org/10.3892/mmr.2021.12403
MLA
Sun, M., Ye, Y., Huang, Y., Yin, W., Yu, Z., Wang, S."Salvianolic acid B improves autophagic dysfunction and decreases the apoptosis of cholesterol crystal‑induced macrophages via inhibiting the Akt/mTOR signaling pathway". Molecular Medicine Reports 24.5 (2021): 763.
Chicago
Sun, M., Ye, Y., Huang, Y., Yin, W., Yu, Z., Wang, S."Salvianolic acid B improves autophagic dysfunction and decreases the apoptosis of cholesterol crystal‑induced macrophages via inhibiting the Akt/mTOR signaling pathway". Molecular Medicine Reports 24, no. 5 (2021): 763. https://doi.org/10.3892/mmr.2021.12403
Copy and paste a formatted citation
x
Spandidos Publications style
Sun M, Ye Y, Huang Y, Yin W, Yu Z and Wang S: Salvianolic acid B improves autophagic dysfunction and decreases the apoptosis of cholesterol crystal‑induced macrophages via inhibiting the Akt/mTOR signaling pathway. Mol Med Rep 24: 763, 2021.
APA
Sun, M., Ye, Y., Huang, Y., Yin, W., Yu, Z., & Wang, S. (2021). Salvianolic acid B improves autophagic dysfunction and decreases the apoptosis of cholesterol crystal‑induced macrophages via inhibiting the Akt/mTOR signaling pathway. Molecular Medicine Reports, 24, 763. https://doi.org/10.3892/mmr.2021.12403
MLA
Sun, M., Ye, Y., Huang, Y., Yin, W., Yu, Z., Wang, S."Salvianolic acid B improves autophagic dysfunction and decreases the apoptosis of cholesterol crystal‑induced macrophages via inhibiting the Akt/mTOR signaling pathway". Molecular Medicine Reports 24.5 (2021): 763.
Chicago
Sun, M., Ye, Y., Huang, Y., Yin, W., Yu, Z., Wang, S."Salvianolic acid B improves autophagic dysfunction and decreases the apoptosis of cholesterol crystal‑induced macrophages via inhibiting the Akt/mTOR signaling pathway". Molecular Medicine Reports 24, no. 5 (2021): 763. https://doi.org/10.3892/mmr.2021.12403
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