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Parallel evaluation of cell‑based phage display panning strategies: Optimized selection and depletion steps result in AML blast‑binding consensus antibodies

  • Authors:
    • Theresa Weber
    • Sibylle Pscherer
    • Ulrike Gamerdinger
    • Andrea Teigler‑Schlegel
    • Natalja Rutz
    • Wolfgang Blau
    • Mathias Rummel
    • Stefan Gattenlöhner
    • Mehmet Kemal Tur
  • View Affiliations / Copyright

    Affiliations: Institute of Pathology, Justus Liebig University Giessen, University Hospital Giessen and Marburg, D‑35392 Giessen, Germany, Department for Hematology, Oncology and Palliative Care, Helios Dr Horst Schmidt Kliniken, D‑65199 Wiesbaden, Germany, Department for Hematology, Justus Liebig University Giessen, University Hospital Giessen and Marburg, D‑35392 Giessen, Germany
    Copyright: © Weber et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 767
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    Published online on: September 3, 2021
       https://doi.org/10.3892/mmr.2021.12407
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Abstract

Phage display technology (PD) is a powerful technique for the generation of tumor‑targeting antibodies. However, there are a number of different selection methods established in different laboratories around the world. Cell‑based PD panning methods using primary tumor cells are particularly heterogeneous between laboratories, which can lead to inconsistent results. Therefore, the present study evaluated different cell‑based PD selection methods regarding their potential to generate acute myeloid leukemia (AML) blast‑binding antibodies. In addition to this evaluation, the present study improved the PD procedure by optimizing selection as well as depletion strategies. To the best of our knowledge, the current study demonstrated for the first time that antigen diversity during the depletion step is of importance for the enrichment of tumor‑targeting phage antibodies. It is demonstrated that medium levels of depletion antigen diversity led to the most promising antibody candidates. In addition, it was determined that purification of blast cells from patients with AML by immunomagnetic separation ameliorated the selection of AML‑binding phages during panning. Furthermore, suggesting a common design‑related mechanism using a ‘single‑pot’ PD library, such as the well‑known Tomlinson single‑chain fragment variable (scFv) library, the present study identified specific binding consensus phage particles in independent panning procedures. By means of these optimized strategies, four promising AML blast‑binding phage particles were isolated and soluble scFv‑Fc (scFv cloned to a fragment crystallizable of an IgG2a mouse antibody) fusion proteins were produced. These scFv‑Fc antibodies bound the surface of AML blasts and were successfully internalized into their cytoplasm, indicating that they are potential immunoconjugate candidates for AML immunotherapy.
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1 

Medinger M, Lengerke C and Passweg J: Novel therapeutic options in acute myeloid leukemia. Leuk Res Rep. 6:39–49. 2016.PubMed/NCBI

2 

Southam CM, Craver LF, Dargeon HW and Burchenal JH: A study of the natural history of acute leukemia with special reference to the duration of the disease and the occurrence of remissions. Cancer. 4:39–59. 1951. View Article : Google Scholar : PubMed/NCBI

3 

Juliusson G, Antunovic P, Derolf A, Lehmann S, Mollgard L, Stockelberg D, Tidefelt U, Wahlin A and Höglund M: Age and acute myeloid leukemia: Real world data on decision to treat and outcomes from the Swedish acute leukemia registry. Blood. 30:4179–4187. 2009. View Article : Google Scholar : PubMed/NCBI

4 

Berger DP, Mertelsmann R and Duyster J; Tumorzentrum Freiburg- CCCF (eds). Das Rote Buch, : Hämatologie und internistische Onkologie. überarbeitete und erweiterte Auflage. Landsberg am Lech: ecomed Medizin. p14002017.

5 

Patel JP, Gönen M, Figueroa ME, Fernandez H, Sun Z, Racevskis J, Van Vlierberghe P, Dolgalev I, Thomas S, Aminova O, et al: Prognostic relevance of integrated genetic profiling in acute myeloid leukemia. N Engl J Med. 22:1079–1089. 2012. View Article : Google Scholar : PubMed/NCBI

6 

Papaemmanuil E, Gerstung M, Bullinger L, Gaidzik VI, Paschka P, Roberts ND, Potter NE, Heuser M, Thol F, Bolli N, et al: Genomic classification and prognosis in acute myeloid leukemia. N Engl J Med. 9:2209–2221. 2016. View Article : Google Scholar : PubMed/NCBI

7 

Cancer Genome Atlas Research Network, ; Ley TJ, Miller C, Ding L, Raphael BJ, Mungall AJ, Robertson AG, Hoadley K, Triche TJ Jr, Laird PW, et al: Genomic and epigenomic landscapes of adult de novo acute myeloid leukemia. N Engl J Med. 30:2059–2074. 2013.PubMed/NCBI

8 

Talati C and Sweet K: Recently approved therapies in acute myeloid leukemia: A complex treatment landscape. Leuk Res. 73:58–66. 2018. View Article : Google Scholar : PubMed/NCBI

9 

Dugan J and Pollyea D: Enasidenib for the treatment of acute myeloid leukemia. Expert Rev Clin Pharmacol. 3:755–760. 2018. View Article : Google Scholar : PubMed/NCBI

10 

Pollyea DA: New drugs for acute myeloid leukemia inspired by genomics and when to use them. Hematology Am Soc Hematol Educ Program. 30:45–50. 2018. View Article : Google Scholar : PubMed/NCBI

11 

Egan PC and Reagan JL: The return of gemtuzumab ozogamicin: A humanized anti-CD33 monoclonal antibody-drug conjugate for the treatment of newly diagnosed acute myeloid leukemia. Onco Targets Ther. 11:8265–8272. 2018. View Article : Google Scholar : PubMed/NCBI

12 

McCafferty J, Griffiths AD, Winter G and Chiswell DJ: Phage antibodies: Filamentous phage displaying antibody variable domains. Nature. 348:552–524. 1990. View Article : Google Scholar : PubMed/NCBI

13 

Breitling F, Dübel S, Seehaus T, Klewinghaus I and Little M: A surface expression vector for antibody screening. Gene. 15:147–153. 1991. View Article : Google Scholar : PubMed/NCBI

14 

Frei JC and Lai JR: Protein and antibody engineering by phage display. Methods Enzymol. 5806:45–87. 2016. View Article : Google Scholar : PubMed/NCBI

15 

Barbas III CF, Burton DR, Scott JK and Silverman GJ: Phage Display: A Laboratory Manual. Cold Spring Harbor Laboratory; New York, NY: 2004, PubMed/NCBI

16 

Smith GP and Petrenko VA: Phage display. Chem Rev. 97:391–410. 1997. View Article : Google Scholar : PubMed/NCBI

17 

Tur MK, Rothe A, Huhn M, Goerres U, Klimka A, Stöcker M, Engert A, Fischer R, Finner R and Barth S: A novel approach for immunization, screening and characterization of selected scFv libraries using membrane fractions of tumor cells. Int J Mol Med. 11:523–527. 2003.PubMed/NCBI

18 

Ten Haaf A, Gattenlöhner S and Tur MK: Antibody selection on FFPE tissue slides. Methods Mol Biol. 1701:381–391. 2018. View Article : Google Scholar : PubMed/NCBI

19 

Fitting J, Blume T, Ten Haaf A, Blau W, Gattenlöhner S, Tur MK and Barth S: Phage display-based generation of novel internalizing antibody fragments for immunotoxin-based treatment of acute myeloid leukemia. MAbs. 7:390–402. 2015. View Article : Google Scholar : PubMed/NCBI

20 

Ljungars A, Svensson C, Carlsson A, Birgersson E, Tornberg UC, Frendéus B, Ohlin M and Mattsson M: Deep mining of complex antibody phage pools generated by cell panning enables discovery of rare antibodies binding new targets and epitopes. Front Pharmacol. 10:8472019. View Article : Google Scholar : PubMed/NCBI

21 

Bakker ABH, van den Oudenrijn S, Bakker AQ, Feller N, van Meijer M, Bia JA, Jongeneelen MA, Visser TJ, Bijl N, Geuijen CA, et al: C-type lectin-like molecule-1: A novel myeloid cell surface marker associated with acute myeloid leukemia. Cancer Res. 15:8443–8450. 2004. View Article : Google Scholar : PubMed/NCBI

22 

Geuijen CAW, Bijl N, Smit RCM, Cox F, Throsby M, Visser TJ, Jongeneelen MA, Bakker AB, Kruisbeek AM, Goudsmit J and de Kruif J: A proteomic approach to tumour target identification using phage display, affinity purification and mass spectrometry. Eur J Cancer. 41:178–187. 1990. View Article : Google Scholar : PubMed/NCBI

23 

ten Haaf A, Pscherer S, Fries K, Barth S, Gattenlöhner S and Tur MK: Phage display-based on-slide selection of tumor-specific antibodies on formalin-fixed paraffin-embedded human tissue biopsies. Immunol Lett. 166:65–78. 2015. View Article : Google Scholar : PubMed/NCBI

24 

Sidney LE, Branch MJ, Dunphy SE, Dua HS and Hopkinson A: Concise review: Evidence for CD34 as a common marker for diverse progenitors. Stem Cells. 32:1380–1289. 2014. View Article : Google Scholar : PubMed/NCBI

25 

Leach RM, Drummond M, Doig A, McKay P, Jackson B and Bain BJ: Practical Flow Cytometry in Haematology: 100 worked examples. John Wiley and Sons, Inc.; Hoboken, NJ: 2015, View Article : Google Scholar

26 

Fitting J, Killian D, Junghanss C, Willenbrock S, Escobar HM, Lange S, Nolte I, Barth S and Tur MK: Generation of recombinant antibody fragments that target canine dendritic cells by phage display technology. Vet Comp Oncol. 9:183–195. 2011. View Article : Google Scholar : PubMed/NCBI

27 

Sánchez-Martín D, Sørensen MD, Lykkemark S, Sanz L, Kristensen P, Ruoslahti E and Álvarez-Vallina L: Selection strategies for anticancer antibody discovery: Searching off the beaten path. Trends Biotechnol. 33:292–301. 2015. View Article : Google Scholar : PubMed/NCBI

28 

Jäger S, Jahnke A, Wilmes T, Adebahr S, Vögtle FN, Delima-Hahn E, Pfeifer D, Berg T, Lübbert M and Trepel M: Leukemia-targeting ligands isolated from phage-display peptide libraries. Leukemia. 21:411–420. 2007. View Article : Google Scholar : PubMed/NCBI

29 

Galili N, Devemy E and Raza A: Isolation of specific and biologically active peptides that bind cells from patients with acute myeloid leukemia (AML). J Hematol Oncol. 10:82008. View Article : Google Scholar : PubMed/NCBI

30 

Karjalainen K, Jaalouk DE, Bueso-Ramos CE, Zurita AJ, Kuniyasu A, Eckhardt BL, Marini FC, Lichtiger B, O'Brien S, Kantarjian HM, et al: Targeting neuropilin-1 in human leukemia and lymphoma. Blood. 20:920–927. 2011. View Article : Google Scholar : PubMed/NCBI

31 

Nodehi SM, Repp R, Kellner C, Bräutigam J, Staudinger M, Schub N, Peipp M, Gramatzki M and Humpe A: Enhanced ADCC activity of affinity maturated and Fc-engineered mini-antibodies directed against the AML stem cell antigen CD96. PLoS One. 7:e424262012. View Article : Google Scholar : PubMed/NCBI

32 

Hoen PA, Jirka SM, ten Broeke BR, Schultes EA, Aguilera B, Pang KH, Heemskerk H, Aartsma-Rus A, van Ommen GJ and den Dunnen JT: Phage display screening without repetitious selection rounds. Anal Biochem. 421:622–631. 2012. View Article : Google Scholar : PubMed/NCBI

33 

Luttmann W, Bratke K, Küpper M and Myrtek D: Der Experimentator: Immunologie. Springer; Heidelberg: 2014, View Article : Google Scholar

34 

Ngai PK, Ackermann F, Wendt H, Savoca R and Bosshard HR: Protein A antibody-capture ELISA (PACE): An ELISA format to avoid denaturation of surface-adsorbed antigens. J Immunol Methods. 158:267–276. 1993. View Article : Google Scholar : PubMed/NCBI

35 

Nikfarjam S, Tohidkia MR, Mehdipour T, Soleimani R, Rahimi AA and Nouri M: Successful application of whole cell panning for isolation of phage antibody fragments specific to differentiated gastric cancer cells. Adv Pharm Bull. 9:624–631. 2019. View Article : Google Scholar : PubMed/NCBI

36 

Jones ML, Alfaleh MA, Kumble S, Zhang S, Osborne GW, Yeh M, Arora N, Hou JJ, Howard CB, Chin DY and Mahler SM: Targeting membrane proteins for antibody discovery using phage display. Sci Rep. 6:262402016. View Article : Google Scholar : PubMed/NCBI

37 

Derda R, Tang S, Li SC, Ng S, Matochko W and Jafari MR: Diversity of phage-displayed libraries of peptides during panning and amplification. Molecules. 21:1776–1803. 2011. View Article : Google Scholar : PubMed/NCBI

38 

Naeim F, Nagesh Rao P, Song SX and Phan RT: Principles of Immunophenotyping. Atlas of Hematopathology. Elsevier; pp. 29–56. 2018, View Article : Google Scholar

39 

Ricart AD: Antibody-drug conjugates of calicheamicin derivative: Gemtuzumab ozogamicin and inotuzumab ozogamicin. Clin Cancer Res. 15:6417–6427. 2011. View Article : Google Scholar : PubMed/NCBI

40 

Vercauteren SM, Zapf R and Sutherland HJ: Primitive AML progenitors from most CD34(+) patients lack CD33 expression but progenitors from many CD34(−) AML patients express CD33. Cytotherapy. 9:194–204. 2007. View Article : Google Scholar : PubMed/NCBI

41 

Matsui H, Takeshita A, Naito K, Shinjo K, Shigeno K, Maekawa M, Yamakawa Y, Tanimoto M, Kobayashi M, Ohnishi K and Ohno R: Reduced effect of gemtuzumab ozogamicin (CMA-676) on P-glycoprotein and/or CD34-positive leukemia cells and its restoration by multidrug resistance modifiers. Leukemia. 16:813–819. 2002. View Article : Google Scholar : PubMed/NCBI

42 

Wells SJ, Bray RA, Stempora LL and Farhi DC: CD117/CD34 expression in leukemic blasts. Am J Clin Pathol. 1:192–195. 1996. View Article : Google Scholar : PubMed/NCBI

43 

Alfaleh MA, Jones ML, Howard CB and Mahler SM: Strategies for selecting membrane protein-specific antibodies using phage display with cell-based panning. Antibodies (Basel). 5:102017. View Article : Google Scholar : PubMed/NCBI

44 

Xu Y, McKenna RW, Wilson KS, Karandikar NJ, Schultz RA and Kroft SH: Immunophenotypic identification of acute myeloid leukemia with monocytic differentiation. Leukemia. 20:1321–1324. 2006. View Article : Google Scholar : PubMed/NCBI

45 

Reader RH, Workman RG, Maddison BC and Gough KC: Advances in the production and batch reformatting of phage antibody libraries. Mol Biotechnol. 61:801–815. 2019. View Article : Google Scholar : PubMed/NCBI

46 

Pokorny NJ, Boulter-Bitzer JI, Hall JC, Trevors JT and Lee H: Inhibition of Cryptosporidium parvum infection of a mammalian cell culture by recombinant scFv antibodies. Antonie Van Leeuwenhoek. 94:353–364. 2008. View Article : Google Scholar : PubMed/NCBI

47 

Wu S, Ke A and Doudna JA: A fast and efficient procedure to produce scFvs specific for large macromolecular complexes. J Immunol Methods. 318:95–101. 2007. View Article : Google Scholar : PubMed/NCBI

48 

Boulter-Bitzer JI, Lee H and Trevors JT: Single-chain variable fragment antibodies selected by phage display against the sporozoite surface antigen P23 of cryptosporidium parvum. J Parasitol. 95:75–81. 2009. View Article : Google Scholar : PubMed/NCBI

49 

Marcus WD, Lindsay SM and Sierks MR: Identification and repair of positive binding antibodies containing randomly generated amber codons from synthetic phage display libraries. Biotechnol Prog. 2:919–922. 2006. View Article : Google Scholar : PubMed/NCBI

50 

Huang W, Samanta M, Crawford SE, Estes MK, Neill FH, Atmar RL and Palzkill T: Identification of human single-chain antibodies with broad reactivity for noroviruses. Protein Eng Des Sel. 27:339–349. 2014. View Article : Google Scholar : PubMed/NCBI

51 

Vodnik M, Zager U, Strukelj B and Lunder M: Phage display: Selecting straws instead of a needle from a haystack. Molecules. 19:790–817. 2011. View Article : Google Scholar : PubMed/NCBI

52 

Thomas WD, Golomb M and Smith GP: Corruption of phage display libraries by target-unrelated clones: Diagnosis and countermeasures. Anal Biochem. 407:237–240. 2010. View Article : Google Scholar : PubMed/NCBI

53 

Kuzmicheva GA, Jayanna PK, Sorokulova IB and Petrenko VA: Diversity and censoring of landscape phage libraries. Protein Eng Des Sel. 22:9–18. 2009. View Article : Google Scholar : PubMed/NCBI

54 

Brammer LA, Bolduc B, Kass JL, Felice KM, Noren CJ and Hall MF: A target-unrelated peptide in an M13 phage display library traced to an advantageous mutation in the gene II ribosome-binding site. Anal Biochem. 373:88–98. 2008. View Article : Google Scholar : PubMed/NCBI

55 

Menendez A and Scott JK: The nature of target-unrelated peptides recovered in the screening of phage-displayed random peptide libraries with antibodies. Anal Biochem. 336:145–157. 2005. View Article : Google Scholar : PubMed/NCBI

56 

Sasso E, D'Avino C, Passariello M, D'Alise AM, Siciliano D, Esposito ML, Froechlich G, Cortese R, Scarselli E, Zambrano N, et al: Massive parallel screening of phage libraries for the generation of repertoires of human immunomodulatory monoclonal antibodies. MAbs. 11:1–13. 2018. View Article : Google Scholar

57 

Harper J, Mao S, Strout P and Kamal A: Selecting an optimal antibody for antibody-drug conjugate therapy: Internalization and intracellular localization. Methods Mol Biol. 1045:41–49, 2013.0. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Weber T, Pscherer S, Gamerdinger U, Teigler‑Schlegel A, Rutz N, Blau W, Rummel M, Gattenlöhner S and Tur MK: Parallel evaluation of cell‑based phage display panning strategies: Optimized selection and depletion steps result in AML blast‑binding consensus antibodies. Mol Med Rep 24: 767, 2021.
APA
Weber, T., Pscherer, S., Gamerdinger, U., Teigler‑Schlegel, A., Rutz, N., Blau, W. ... Tur, M.K. (2021). Parallel evaluation of cell‑based phage display panning strategies: Optimized selection and depletion steps result in AML blast‑binding consensus antibodies. Molecular Medicine Reports, 24, 767. https://doi.org/10.3892/mmr.2021.12407
MLA
Weber, T., Pscherer, S., Gamerdinger, U., Teigler‑Schlegel, A., Rutz, N., Blau, W., Rummel, M., Gattenlöhner, S., Tur, M. K."Parallel evaluation of cell‑based phage display panning strategies: Optimized selection and depletion steps result in AML blast‑binding consensus antibodies". Molecular Medicine Reports 24.5 (2021): 767.
Chicago
Weber, T., Pscherer, S., Gamerdinger, U., Teigler‑Schlegel, A., Rutz, N., Blau, W., Rummel, M., Gattenlöhner, S., Tur, M. K."Parallel evaluation of cell‑based phage display panning strategies: Optimized selection and depletion steps result in AML blast‑binding consensus antibodies". Molecular Medicine Reports 24, no. 5 (2021): 767. https://doi.org/10.3892/mmr.2021.12407
Copy and paste a formatted citation
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Spandidos Publications style
Weber T, Pscherer S, Gamerdinger U, Teigler‑Schlegel A, Rutz N, Blau W, Rummel M, Gattenlöhner S and Tur MK: Parallel evaluation of cell‑based phage display panning strategies: Optimized selection and depletion steps result in AML blast‑binding consensus antibodies. Mol Med Rep 24: 767, 2021.
APA
Weber, T., Pscherer, S., Gamerdinger, U., Teigler‑Schlegel, A., Rutz, N., Blau, W. ... Tur, M.K. (2021). Parallel evaluation of cell‑based phage display panning strategies: Optimized selection and depletion steps result in AML blast‑binding consensus antibodies. Molecular Medicine Reports, 24, 767. https://doi.org/10.3892/mmr.2021.12407
MLA
Weber, T., Pscherer, S., Gamerdinger, U., Teigler‑Schlegel, A., Rutz, N., Blau, W., Rummel, M., Gattenlöhner, S., Tur, M. K."Parallel evaluation of cell‑based phage display panning strategies: Optimized selection and depletion steps result in AML blast‑binding consensus antibodies". Molecular Medicine Reports 24.5 (2021): 767.
Chicago
Weber, T., Pscherer, S., Gamerdinger, U., Teigler‑Schlegel, A., Rutz, N., Blau, W., Rummel, M., Gattenlöhner, S., Tur, M. K."Parallel evaluation of cell‑based phage display panning strategies: Optimized selection and depletion steps result in AML blast‑binding consensus antibodies". Molecular Medicine Reports 24, no. 5 (2021): 767. https://doi.org/10.3892/mmr.2021.12407
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