Lycium barbarum polysaccharide protects ARPE‑19 cells against H<sub>2</sub>O<sub>2</sub>‑induced oxidative stress via the Nrf2/HO‑1 pathway

Liang,Ran; Zhao,Qi; Zhu,Qing; He,Xin; Gao,Mingjun; Wang,Yiru

doi:10.3892/mmr.2021.12409

Lycium barbarum polysaccharide protects ARPE‑19 cells against H₂O₂‑induced oxidative stress via the Nrf2/HO‑1 pathway

Authors:
- Ran Liang
- Qi Zhao
- Qing Zhu
- Xin He
- Mingjun Gao
- Yiru Wang
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Affiliations: Department of Ophthalmology, The Second Hospital of Dalian Medical University, Dalian, Liaoning 116021, P.R. China
Published online on: September 3, 2021 https://doi.org/10.3892/mmr.2021.12409
Article Number: 769
Copyright: © Liang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Age‑related macular degeneration (AMD) is a global health problem. Lycium barbarum polysaccharide (LBP), a traditional Chinese herbal medicine, has been proven to be effective against several eye diseases. However, only a few studies have investigated the effectiveness of LBP for AMD. In the present study, the human retinal epithelial cell line, ARPE‑19, was pretreated with LBP for 24 h before exposure to H₂O₂ (500 µM). Cell viability was assessed, and a series of oxidative and antioxidant indicators were evaluated to determine the influence of LBP on H2O2‑triggered oxidative stress. The present study also determined the apoptosis status, as well as the expression levels of apoptotic proteins and nuclear factor erythroid 2‑related factor 2 (Nrf2)/heme oxygenase‑1 (HO‑1) pathway proteins. The present study aimed to determine the protective role for LBP pretreatment and its underlying molecular mechanism. The results of the present study suggest that pretreatment of ARPE‑19 cells with LBP exhibit high efficacy at reducing oxidative damage and inhibiting cell apoptosis. Furthermore, LBP may modulate the expression of proteins involved in the apoptotic pathway and activate the Nrf2 signaling pathway.

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