Lycium barbarum polysaccharide protects ARPE‑19 cells against H<sub>2</sub>O<sub>2</sub>‑induced oxidative stress via the Nrf2/HO‑1 pathway

Liang,Ran; Zhao,Qi; Zhu,Qing; He,Xin; Gao,Mingjun; Wang,Yiru

doi:10.3892/mmr.2021.12409

November-2021 Volume 24 Issue 5

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November-2021 Volume 24 Issue 5

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Article Open Access

Lycium barbarum polysaccharide protects ARPE‑19 cells against H₂O₂‑induced oxidative stress via the Nrf2/HO‑1 pathway

Authors:
- Ran Liang
- Qi Zhao
- Qing Zhu
- Xin He
- Mingjun Gao
- Yiru Wang
View Affiliations / Copyright

Affiliations: Department of Ophthalmology, The Second Hospital of Dalian Medical University, Dalian, Liaoning 116021, P.R. China

Copyright: © Liang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Article Number: 769
|
Published online on: September 3, 2021

https://doi.org/10.3892/mmr.2021.12409
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Abstract

Age‑related macular degeneration (AMD) is a global health problem. Lycium barbarum polysaccharide (LBP), a traditional Chinese herbal medicine, has been proven to be effective against several eye diseases. However, only a few studies have investigated the effectiveness of LBP for AMD. In the present study, the human retinal epithelial cell line, ARPE‑19, was pretreated with LBP for 24 h before exposure to H₂O₂ (500 µM). Cell viability was assessed, and a series of oxidative and antioxidant indicators were evaluated to determine the influence of LBP on H2O2‑triggered oxidative stress. The present study also determined the apoptosis status, as well as the expression levels of apoptotic proteins and nuclear factor erythroid 2‑related factor 2 (Nrf2)/heme oxygenase‑1 (HO‑1) pathway proteins. The present study aimed to determine the protective role for LBP pretreatment and its underlying molecular mechanism. The results of the present study suggest that pretreatment of ARPE‑19 cells with LBP exhibit high efficacy at reducing oxidative damage and inhibiting cell apoptosis. Furthermore, LBP may modulate the expression of proteins involved in the apoptotic pathway and activate the Nrf2 signaling pathway.

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1	Mitchell P, Liew G, Gopinath B and Wong TY: Age-related macular degeneration. Lancet. 392:1147–1159. 2018. View Article : Google Scholar : PubMed/NCBI
2	Yang M, So KF, Lo ACY and Lam WC: The effect of Lycium barbarum polysaccharides on pyroptosis-associated amyloid β1-40 oligomers-induced adult retinal pigment epithelium 19 cell damage. Int J Mol Sci. 21:46582020. View Article : Google Scholar : PubMed/NCBI
3	Bhutto IA, Baba T, Merges C, McLeod DS and Lutty GA: Low nitric oxide synthases (NOSs) in eyes with age-related macular degeneration (AMD). Exp Eye Res. 90:155–167. 2010. View Article : Google Scholar : PubMed/NCBI
4	Strauss O: The retinal pigment epithelium in visual function. Physiol Rev. 85:845–881. 2005. View Article : Google Scholar : PubMed/NCBI
5	Anderson RE, Rapp LM and Wiegand RD: Lipid peroxidation and retinal degeneration. Curr Eye Res. 3:223–227. 1984. View Article : Google Scholar : PubMed/NCBI
6	Catalá A: An overview of lipid peroxidation with emphasis in outer segments of photoreceptors and the chemiluminescence assay. Int J Biochem Cell Biol. 38:1482–1495. 2006. View Article : Google Scholar : PubMed/NCBI
7	Cai J, Nelson KC, Wu M, Sternberg P Jr and Jones DP: Oxidative damage and protection of the RPE. Prog Retin Eye Res. 19:205–221. 2000. View Article : Google Scholar : PubMed/NCBI
8	Bellezza I, Giambanco I, Minelli A and Donato R: Nrf2-Keap1 signaling in oxidative and reductive stress. Biochim Biophys Acta Mol Cell Res. 1865:721–733. 2018. View Article : Google Scholar : PubMed/NCBI
9	Kobayashi M and Yamamoto M: Molecular mechanisms activating the Nrf2-Keap1 pathway of antioxidant gene regulation. Antioxid Redox Signal. 7:385–394. 2005. View Article : Google Scholar : PubMed/NCBI
10	Shelton LM, Kevin Park B and Copple IM: Role of Nrf2 in protection against acute kidney injury. Kidney Int. 84:1090–1095. 2013. View Article : Google Scholar : PubMed/NCBI
11	Zhou J, Chen F, Yan A and Xia X: Madecassoside protects retinal pigment epithelial cells against hydrogen peroxide-induced oxidative stress and apoptosis through the activation of Nrf2/HO-1 pathway. Biosci Rep. 40:BSR201943472020. View Article : Google Scholar : PubMed/NCBI
12	Cameron BD, Sekhar KR, Ofori M and Freeman ML: The role of Nrf2 in the response to normal tissue radiation injury. Radiat Res. 190:99–106. 2018. View Article : Google Scholar : PubMed/NCBI
13	Liu W, Liu Y, Zhu R, Yu J, Lu W, Pan C, Yao W and Gao X: Structure characterization, chemical and enzymatic degradation, and chain conformation of an acidic polysaccharide from Lycium barbarum L. Carbohydr Polym. 147:114–124. 2016. View Article : Google Scholar : PubMed/NCBI
14	Qi B, Ji Q, Wen Y, Liu L, Guo X, Hou G, Wang G and Zhong J: Lycium barbarum polysaccharides protect human lens epithelial cells against oxidative stress-induced apoptosis and senescence. PLoS One. 9:e1102752014. View Article : Google Scholar : PubMed/NCBI
15	Pan H, Shi Z, Yang TG, Yu LM and Xu AL: The protective effects of lycium barbarum polysaccharides on retinal neurons in diabetic rats and its mechanism. Zhongguo Ying Yong Sheng Li Xue Za Zhi. 35:55–59. 2019.(In Chinese). PubMed/NCBI
16	Curcio CA, Zanzottera EC, Ach T, Balaratnasingam C and Freund KB: Activated retinal pigment epithelium, an optical coherence tomography biomarker for progression in age-related macular degeneration. Invest Ophthalmol Vis Sci. 58:BIO211–BIO226. 2017.PubMed/NCBI
17	Kopitz J, Holz FG, Kaemmerer E and Schutt F: Lipids and lipid peroxidation products in the pathogenesis of age-related macular degeneration. Biochimie. 86:825–831. 2004. View Article : Google Scholar : PubMed/NCBI
18	Li S, Gaur U, Chong CM, Lin S, Fang J, Zeng Z, Wang H and Zheng W: Berberine protects human retinal pigment epithelial cells from hydrogen peroxide-induced oxidative damage through activation of AMPK. Int J Mol Sci. 19:17362018. View Article : Google Scholar : PubMed/NCBI
19	Golestaneh N, Chu Y, Xiao YY, Stoleru GL and Theos AC: Dysfunctional autophagy in RPE, a contributing factor in age-related macular degeneration. Cell Death Dis. 8:e25372017. View Article : Google Scholar : PubMed/NCBI
20	Golestaneh N, Chu Y, Cheng SK, Cao H, Poliakov E and Berinstein DM: Repressed SIRT1/PGC-1α pathway and mitochondrial disintegration in iPSC-derived RPE disease model of age-related macular degeneration. J Transl Med. 14:3442016. View Article : Google Scholar : PubMed/NCBI
21	Mi XS, Chiu K, Van G, Leung JW, Lo AC, Chung SK, Chang RC and So KF: Effect of Lycium barbarum Polysaccharides on the expression of endothelin-1 and its receptors in an ocular hypertension model of rat glaucoma. Neural Regen Res. 7:645–651. 2012.PubMed/NCBI
22	Li SY, Yang D, Yeung CM, Yu WY, Chang RC, So KF, Wong D and Lo AC: Lycium barbarum polysaccharides reduce neuronal damage, blood-retinal barrier disruption and oxidative stress in retinal ischemia/reperfusion injury. PLoS One. 6:e163802011. View Article : Google Scholar : PubMed/NCBI
23	Yao Q, Yang Y, Lu X, Zhang Q, Luo M, Li PA and Pan Y: Lycium barbarum polysaccharides improve retinopathy in diabetic sprague-dawley rats. Evid Based Complement Alternat Med. 2018:79432122018. View Article : Google Scholar : PubMed/NCBI
24	Varoni MV, Pasciu V, Gadau SD, Baralla E, Serra E, Palomba D and Demontis MP: Possible antioxidant effect of Lycium barbarum polysaccharides on hepatic cadmium-induced oxidative stress in rats. Environ Sci Pollut Res Int. 24:2946–2955. 2017. View Article : Google Scholar : PubMed/NCBI
25	Chen L, Li W, Qi D and Wang D: Lycium barbarum polysaccharide protects against LPS-induced ARDS by inhibiting apoptosis, oxidative stress, and inflammation in pulmonary endothelial cells. Free Radic Res. 52:480–490. 2018. View Article : Google Scholar : PubMed/NCBI
26	Schoppet M, Tailhades J, Kulkarni K and Cryle MJ: Precursor manipulation in glycopeptide antibiotic biosynthesis: Are β-amino acids compatible with the oxidative cyclization cascade? J Org Chem. 83:7206–7214. 2018. View Article : Google Scholar : PubMed/NCBI
27	Kaczara P, Sarna T and Burke JM: Dynamics of H2O2 availability to ARPE-19 cultures in models of oxidative stress. Free Radic Biol Med. 48:1064–1070. 2010. View Article : Google Scholar : PubMed/NCBI
28	Geiger RC, Waters CM, Kamp DW and Glucksberg MR: KGF prevents oxygen-mediated damage in ARPE-19 cells. Invest Ophthalmol Vis Sci. 46:3435–3442. 2005. View Article : Google Scholar : PubMed/NCBI
29	Zareba M, Raciti MW, Henry MM, Sarna T and Burke JM: Oxidative stress in ARPE-19 cultures: Do melanosomes confer cytoprotection? Free Radic Biol Med. 40:87–100. 2006. View Article : Google Scholar : PubMed/NCBI
30	Zhao H, Wang R, Ye M and Zhang L: Genipin protects against H2O2-induced oxidative damage in retinal pigment epithelial cells by promoting Nrf2 signaling. Int J Mol Med. 43:936–944. 2019.PubMed/NCBI
31	Pintea A, Rugină DO, Pop R, Bunea A and Socaciu C: Xanthophylls protect against induced oxidation in cultured human retinal pigment epithelial cells. J Food Compos Anal. 24:830–836. 2011. View Article : Google Scholar
32	Musat O, Ochinciuc U, Gutu T, Cristescu TR and Coman C: Pathophysiology and treatment of ARMD. Oftalmologia. 56:45–50. 2012.(In Romanian). PubMed/NCBI
33	Qu S, Zhang C, Liu D, Wu J, Tian H, Lu L, Xu GT, Liu F and Zhang J: Metformin protects ARPE-19 cells from glyoxal-induced oxidative stress. Oxid Med Cell Longev. 2020:17409432020.PubMed/NCBI
34	Sakai E, Shimada-Sugawara M, Yamaguchi Y, Sakamoto H, Fumimoto R, Fukuma Y, Nishishita K, Okamoto K and Tsukuba T: Fisetin inhibits osteoclastogenesis through prevention of RANKL-induced ROS production by Nrf2-mediated up-regulation of phase II antioxidant enzymes. J Pharmacol Sci. 121:288–298. 2013. View Article : Google Scholar : PubMed/NCBI
35	Jiang P, Chen L, Sun J, Li J, Xu J, Liu W, Feng F and Qu W: Chotosan ameliorates cognitive impairment and hippocampus neuronal loss in experimental vascular dementia via activating the Nrf2-mediated antioxidant pathway. J Pharmacol Sci. 139:105–111. 2019. View Article : Google Scholar : PubMed/NCBI
36	Xiong GF, Li DW, Zheng MB and Liu SC: The effects of Lycium barbarum polysaccharide (LBP) in a mouse model of cerulein-induced acute pancreatitis. Med Sci Monit. 25:3880–3886. 2019. View Article : Google Scholar : PubMed/NCBI
37	Huang Y, Zhou F, Shen C, Wang H and Xiao Y: LBP reduces theinflammatory injuryof kidney in septic rat and regulates the Keap1-Nrf2/ARE signaling pathway1. Acta Cir Bras. 34:e201900100000032019. View Article : Google Scholar : PubMed/NCBI
38	Chen B, Lu Y, Chen Y and Cheng J: The role of Nrf2 in oxidative stress-induced endothelial injuries. J Endocrinol. 225:R83–R99. 2015. View Article : Google Scholar : PubMed/NCBI
39	Hiramatsu K, Tsuneyoshi T, Ogawa T and Morihara N: Aged garlic extract enhances heme oxygenase-1 and glutamate-cysteine ligase modifier subunit expression via the nuclear factor erythroid 2-related factor 2-antioxidant response element signaling pathway in human endothelial cells. Nutr Res. 36:143–149. 2016. View Article : Google Scholar : PubMed/NCBI
40	Hao Y, Liu J, Wang Z, Yu LL and Wang J: Piceatannol protects human retinal pigment epithelial cells against hydrogen peroxide induced oxidative stress and apoptosis through modulating PI3K/Akt signaling pathway. Nutrients. 11:15152019. View Article : Google Scholar : PubMed/NCBI
41	Cao S, Du J and Hei Q: Lycium barbarum polysaccharide protects against neurotoxicity via the Nrf2-HO-1 pathway. Exp Ther Med. 14:4919–4927. 2017.PubMed/NCBI

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