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Tong‑fu‑li‑fei decoction attenuates immunosuppression to protect the intestinal‑mucosal barrier in sepsis by inhibiting the PD‑1/PD‑L1 signaling pathway

  • Authors:
    • Li Chen
    • Lan Li
    • Suzhao Zou
    • Qianhua Liao
    • Bo Lv
  • View Affiliations / Copyright

    Affiliations: Department of Intensive Care Unit, First Affiliated Hospital of Guizhou University of Chinese Medicine, Guiyang, Guizhou 550001, P.R. China, Department of Intensive Care Unit, First Affiliated Hospital of Guizhou University of Chinese Medicine, Guiyang, Guizhou 550001, P.R. China
    Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 840
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    Published online on: October 11, 2021
       https://doi.org/10.3892/mmr.2021.12480
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Abstract

The aim of the present study was to investigate the therapeutic effects of Tong‑fu‑li‑fei (TFL) decoction on sepsis‑induced injury to the intestinal mucosal barrier and the underlying mechanism. Cecal ligation and puncture (CLP) was used to establish a sepsis model in rats. The post‑surgery death of the rats was recorded to calculate the survival rate. A 4‑kD fluorescein isothiocyanate (FITC)‑dextran assay was used to evaluate the intestinal permeability of the rats. The pathological state of the intestine tissues was detected by hematoxylin and eosin staining and the ultrastructural changes in the endometrium were evaluated by transmission electron microscopy. Enzyme‑linked immunosorbent assay was used to determine the concentrations of interleukin (IL)‑6 and tumor necrosis factor (TNF)‑α in the intestinal tissues and cells. The expression levels of SHP‑2 and PI3K were detected by reverse transcription‑quantitative PCR and western blotting. Sorting by flow cytometry was used to obtain pure dendritic cells (DC), CD8+ T cells and natural killer cells. Western blotting was used to evaluate the expression levels of phosphorylated (p)‑AKT and AKT. The results demonstrated that the significantly decreased survival rate caused by CLP surgery was elevated by glutamine (Gln) and TFL treatment. Intestinal permeability was increased by CLP, and greatly suppressed by Gln or TFL treatment. Histopathological changes in the intestinal tissues, such as thinner barrier and atrophied mucosa, and ultrastructure changes such as sharply decreased microvilli and mitochondria dropsy, were observed on sepsis animals; these effects were ameliorated by the introduction of Gln or TFL. The upregulation of SHP‑2, PI3K and p‑AKT induced by CLP was reversed by TFL. The release of IL‑6 and TNF‑α was elevated and the expression of SHP‑2, PI3K and p‑AKT was suppressed in the co‑cultural system of DC cells and CD8+ T cells by TFL. Overall, TFL decoction may attenuate immunosuppression to protect intestinal mucosal barrier in sepsis via inhibiting the programmed death1/programmed cell death ligand 1 signal pathway.
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1 

Uchimido R, Schmidt EP and Shapiro NI: The glycocalyx: A novel diagnostic and therapeutic target in sepsis. Crit Care. 23:162019. View Article : Google Scholar

2 

Riedemann NC, Guo RF and Ward PA: The enigma of sepsis. J Clin Invest. 112:460–467. 2003. View Article : Google Scholar

3 

Osuchowski MF, Welch K, Siddiqui J and Remick DG: Circulating cytokine/inhibitor profiles reshape the understanding of the SIRS/CARS continuum in sepsis and predict mortality. J Immunol. 177:1967–1974. 2006. View Article : Google Scholar

4 

Gotts JE and Matthay MA: Sepsis: Pathophysiology and clinical management. BMJ. 353:i15852016. View Article : Google Scholar

5 

Hotchkiss RS, Tinsley KW, Swanson PE, Schmieg RE Jr, Hui JJ, Chang KC, Osborne DF, Freeman BD, Cobb JP, Buchman TG and Karl IE: Sepsis-induced apoptosis causes progressive profound depletion of B and CD4+ T lymphocytes in humans. J Immunol. 166:6952–6963. 2001. View Article : Google Scholar

6 

Munoz C, Carlet J, Fitting C, Misset B, Bleriot JP and Cavaillon JM: Dysregulation of in vitro cytokine production by monocytes during sepsis. J Clin Invest. 88:1747–1754. 1991. View Article : Google Scholar

7 

Wang HW, Yang W, Gao L, Kang JR, Qin JJ, Liu YP and Lu JY: Adoptive transfer of bone marrow-derived dendritic cells decreases inhibitory and regulatory T-cell differentiation and improves survival in murine polymicrobial sepsis. Immunology. 145:50–59. 2015. View Article : Google Scholar

8 

Bouras M, Asehnoune K and Roquilly A: Contribution of dendritic cell responses to sepsis-induced immunosuppression and to susceptibility to secondary pneumonia. Front Immunol. 9:25902018. View Article : Google Scholar

9 

Manjuck J, Saha DC, Astiz M, Eales LJ and Rackow EC: Decreased response to recall antigens is associated with depressed costimulatory receptor expression in septic critically ill patients. J Lab Clin Med. 135:153–160. 2000. View Article : Google Scholar

10 

Le Tulzo Y, Pangault C, Amiot L, Guilloux V, Tribut O, Arvieux C, Camus C, Fauchet R, Thomas R and Drénou B: Monocyte human leukocyte antigen-DR transcriptional downregulation by cortisol during septic shock. Am J Respir Crit Care Med. 169:1144–1151. 2004. View Article : Google Scholar

11 

Ishida Y, Agata Y, Shibahara K and Honjo T: Induced expression of PD-1, a novel member of the immunoglobulin gene superfamily, upon programmed cell death. EMBO J. 11:3887–3895. 1992. View Article : Google Scholar

12 

Keir ME, Butte MJ, Freeman GJ and Sharpe AH: PD-1 and its ligands in tolerance and immunity. Annu Rev Immunol. 26:677–704. 2008. View Article : Google Scholar

13 

Naidoo J, Schindler K, Querfeld C, Busam K, Cunningham J, Page DB, Postow MA, Weinstein A, Lucas AS, Ciccolini KT, et al: Autoimmune bullous skin disorders with immune checkpoint inhibitors targeting PD-1 and PD-L1. Cancer Immunol Res. 4:383–389. 2016. View Article : Google Scholar

14 

Dai S, Jia R, Zhang X, Fang Q and Huang L: The PD-1/PD-Ls pathway and autoimmune diseases. Cell Immunol. 290:72–79. 2014. View Article : Google Scholar

15 

Brunner-Weinzierl MC and Rudd CE: CTLA-4 and PD-1 control of T-Cell motility and migration: Implications for tumor immunotherapy. Front Immunol. 9:27372018. View Article : Google Scholar

16 

Chen L, Li L, Han Y, Lv B, Zou S and Yu Q: Tong-fu-li-fei decoction exerts a protective effect on intestinal barrier of sepsis in rats through upregulating ZO-1/occludin/claudin-1 expression. J Pharmacol Sci. 143:89–96. 2020. View Article : Google Scholar

17 

Fu J, Li G, Wu X and Zang B: Sodium butyrate ameliorates intestinal injury and improves survival in a rat model of cecal ligation and puncture-induced sepsis. Inflammation. 42:1276–1286. 2019. View Article : Google Scholar

18 

Rendon JL, Li X, Akhtar S and Choudhry MA: Interleukin-22 modulates gut epithelial and immune barrier functions following acute alcohol exposure and burn injury. Shock. 39:11–18. 2013. View Article : Google Scholar

19 

Ke J, Bian X, Liu H, Li B and Huo R: Edaravone reduces oxidative stress and intestinal cell apoptosis after burn through up-regulating miR-320 expression. Mol Med. 25:542019. View Article : Google Scholar

20 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar

21 

Wichterman KA, Baue AE and Chaudry IH: Sepsis and septic shock-a review of laboratory models and a proposal. J Surg Res. 29:189–201. 1980. View Article : Google Scholar

22 

Singer M, Deutschman CS, Seymour CW, Shankar-Hari M, Annane D, Bauer M, Bellomo R, Bernard GR, Chiche JD, Coopersmith CM, et al: The Third International consensus definitions for sepsis and septic shock (Sepsis-3). JAMA. 315:801–810. 2016. View Article : Google Scholar

23 

Shankar-Hari M, Harrison DA, Rubenfeld GD and Rowan K: Epidemiology of sepsis and septic shock in critical care units: Comparison between sepsis-2 and sepsis-3 populations using a National critical care database. Br J Anaesth. 119:626–636. 2017. View Article : Google Scholar

24 

Thomas H: Sepsis: Bile acids promote inflammation in cholestasis-associated sepsis. Nat Rev Gastroenterol Hepatol. 14:324–325. 2017. View Article : Google Scholar

25 

Rosenthal MD and Moore FA: Persistent inflammation, immunosuppression, and catabolism: Evolution of multiple organ dysfunction. Surg Infect (Larchmt). 17:167–172. 2016. View Article : Google Scholar

26 

Zhu Y, Wang Y, Teng W, Shan Y, Yi S, Zhu S and Li Y: Role of aquaporin-3 in intestinal injury induced by sepsis. Biol Pharm Bull. 42:1641–1650. 2019. View Article : Google Scholar

27 

Chang X, Wang LL, Lian SJ, Tang Q, Chen P and Wang H: Effect of oral glutamine on intestinal barrier function in young rats with endotoxemia. Zhongguo Dang Dai Er Ke Za Zhi. 12:809–811. 2010.(In Chinese).

28 

Meena AS, Shukla PK, Sheth P and Rao R: EGF receptor plays a role in the mechanism of glutamine-mediated prevention of alcohol-induced gut barrier dysfunction and liver injury. J Nutr Biochem. 64:128–143. 2019. View Article : Google Scholar

29 

Chao CY, Sung PJ, Wang WH and Kuo YH: Anti-inflammatory effect of Momordica charantia in sepsis mice. Molecules. 19:12777–12788. 2014. View Article : Google Scholar

30 

Venet F, Rimmele T and Monneret G: Management of sepsis-induced immunosuppression. Crit Care Clin. 34:97–106. 2018. View Article : Google Scholar

31 

Venet F and Monneret G: Advances in the understanding and treatment of sepsis-induced immunosuppression. Nat Rev Nephrol. 14:121–137. 2018. View Article : Google Scholar

32 

Roquilly A, McWilliam HEG, Jacqueline C, Tian Z, Cinotti R, Rimbert M, Wakim L, Caminschi I, Lahoud MH, Belz GT, et al: Local modulation of antigen-presenting cell development after resolution of pneumonia induces long-term susceptibility to secondary infections. Immunity. 47:135–147 e5. 2017. View Article : Google Scholar

33 

Chang KC, Burnham CA, Compton SM, Rasche DP, Mazuski RJ, McDonough JS, Unsinger J, Korman AJ, Green JM and Hotchkiss RS: Blockade of the negative co-stimulatory molecules PD-1 and CTLA-4 improves survival in primary and secondary fungal sepsis. Crit Care. 17:R852013. View Article : Google Scholar

34 

Chang K, Svabek C, Vazquez-Guillamet C, Sato B, Rasche D, Wilson S, Robbins P, Ulbrandt N, Suzich J, Green J, et al: Targeting the programmed cell death 1: Programmed cell death ligand 1 pathway reverses T cell exhaustion in patients with sepsis. Crit Care. 18:R32014. View Article : Google Scholar

35 

Li J, Jie HB, Lei Y, Gildener-Leapman N, Trivedi S, Green T, Kane LP and Ferris RL: PD-1/SHP-2 inhibits Tc1/Th1 phenotypic responses and the activation of T cells in the tumor microenvironment. Cancer Res. 75:508–518. 2015. View Article : Google Scholar

36 

Zhao R, Song Y, Wang Y, Huang Y, Li Z, Cui Y, Yi M, Xia L, Zhuang W, Wu X and Zhou Y: PD-1/PD-L1 blockade rescue exhausted CD8+ T cells in gastrointestinal stromal tumours via the PI3K/Akt/mTOR signalling pathway. Cell Prolif. 52:e125712019. View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Chen L, Li L, Zou S, Liao Q and Lv B: Tong‑fu‑li‑fei decoction attenuates immunosuppression to protect the intestinal‑mucosal barrier in sepsis by inhibiting the PD‑1/PD‑L1 signaling pathway. Mol Med Rep 24: 840, 2021.
APA
Chen, L., Li, L., Zou, S., Liao, Q., & Lv, B. (2021). Tong‑fu‑li‑fei decoction attenuates immunosuppression to protect the intestinal‑mucosal barrier in sepsis by inhibiting the PD‑1/PD‑L1 signaling pathway. Molecular Medicine Reports, 24, 840. https://doi.org/10.3892/mmr.2021.12480
MLA
Chen, L., Li, L., Zou, S., Liao, Q., Lv, B."Tong‑fu‑li‑fei decoction attenuates immunosuppression to protect the intestinal‑mucosal barrier in sepsis by inhibiting the PD‑1/PD‑L1 signaling pathway". Molecular Medicine Reports 24.6 (2021): 840.
Chicago
Chen, L., Li, L., Zou, S., Liao, Q., Lv, B."Tong‑fu‑li‑fei decoction attenuates immunosuppression to protect the intestinal‑mucosal barrier in sepsis by inhibiting the PD‑1/PD‑L1 signaling pathway". Molecular Medicine Reports 24, no. 6 (2021): 840. https://doi.org/10.3892/mmr.2021.12480
Copy and paste a formatted citation
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Spandidos Publications style
Chen L, Li L, Zou S, Liao Q and Lv B: Tong‑fu‑li‑fei decoction attenuates immunosuppression to protect the intestinal‑mucosal barrier in sepsis by inhibiting the PD‑1/PD‑L1 signaling pathway. Mol Med Rep 24: 840, 2021.
APA
Chen, L., Li, L., Zou, S., Liao, Q., & Lv, B. (2021). Tong‑fu‑li‑fei decoction attenuates immunosuppression to protect the intestinal‑mucosal barrier in sepsis by inhibiting the PD‑1/PD‑L1 signaling pathway. Molecular Medicine Reports, 24, 840. https://doi.org/10.3892/mmr.2021.12480
MLA
Chen, L., Li, L., Zou, S., Liao, Q., Lv, B."Tong‑fu‑li‑fei decoction attenuates immunosuppression to protect the intestinal‑mucosal barrier in sepsis by inhibiting the PD‑1/PD‑L1 signaling pathway". Molecular Medicine Reports 24.6 (2021): 840.
Chicago
Chen, L., Li, L., Zou, S., Liao, Q., Lv, B."Tong‑fu‑li‑fei decoction attenuates immunosuppression to protect the intestinal‑mucosal barrier in sepsis by inhibiting the PD‑1/PD‑L1 signaling pathway". Molecular Medicine Reports 24, no. 6 (2021): 840. https://doi.org/10.3892/mmr.2021.12480
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