Puerarin alleviates the ototoxicity of gentamicin by inhibiting the mitochondria‑dependent apoptosis pathway

Niu,Ping; Sun,Yuxuan; Wang,Shiyi; Li,Guang; Tang,Xiaomin; Sun,Jiaqiang; Pan,Chunchen; Sun,Jingwu

doi:10.3892/mmr.2021.12491

Puerarin alleviates the ototoxicity of gentamicin by inhibiting the mitochondria‑dependent apoptosis pathway

Authors:
- Ping Niu
- Yuxuan Sun
- Shiyi Wang
- Guang Li
- Xiaomin Tang
- Jiaqiang Sun
- Chunchen Pan
- Jingwu Sun
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Affiliations: Department of Otolaryngology‑Head and Neck Surgery, Anhui Provincial Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong 250012, P.R. China, Department of Otolaryngology‑Head and Neck Surgery, Anhui Provincial Hospital, Hefei, Anhui 230001, P.R. China, Department of Otolaryngology‑Head and Neck Surgery, Affiliated Hospital of Yangzhou University, Yangzhou, Jiangsu 225001, P.R. China
Published online on: October 13, 2021 https://doi.org/10.3892/mmr.2021.12491
Article Number: 851
Copyright: © Niu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Gentamicin (GM) is a commonly used antibiotic, and ototoxicity is one of its side effects. Puerarin (PU) is an isoflavone in kudzu roots that exerts a number of pharmacological effects, including antioxidative and free radical scavenging effects. The present study investigated whether PU could protect against GM‑induced ototoxicity in C57BL/6J mice and House Ear Institute‑Organ of Corti 1 (HEI‑OC1) cells. C57BL/6J mice and HEI‑OC1 cells were used to establish models of GM‑induced ototoxicity in this study. Auditory brainstem responses were measured to assess hearing thresholds, and microscopy was used to observe the morphology of cochlear hair cells after fluorescent staining. Cell viability was examined with Cell Counting Kit‑8 assays. To evaluate cell apoptosis and reactive oxygen species (ROS) production, TUNEL assays, reverse transcription‑quantitative PCR, DCFH‑DA staining, JC‑1 staining and western blotting were performed. PU protected against GM‑induced hearing damage in C57BL/6J mice. PU ameliorated the morphological changes of mouse cochlear hair cells and reduced the apoptosis rate of HEI‑OC1 cells after GM‑mediated damage. GM‑induced ototoxicity may be closely related to the upregulation of p53 expression and the activation of endogenous mitochondrial apoptosis pathways, and PU could protect cochlear hair cells from GM‑mediated damage by reducing the production of ROS and inhibiting the mitochondria‑dependent apoptosis pathway.

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