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MicroRNA‑200c‑3p suppresses intervertebral disc degeneration by targeting RAP2C/ERK signaling

  • Authors:
    • Jianping Cao
    • Meng Jiang
    • Huafeng Ren
    • Kai Xu
  • View Affiliations / Copyright

    Affiliations: Department of Anesthesiology, Qingdao No. 6 People's Hospital, Qingdao, Shandong 266033, P.R. China, Department of Orthopedics, Qingdao No. 6 People's Hospital, Qingdao, Shandong 266033, P.R. China, Department of Functional Inspection, Qingdao Haici Medical Treatment Group, Qingdao, Shandong 266033, P.R. China
    Copyright: © Cao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 865
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    Published online on: October 20, 2021
       https://doi.org/10.3892/mmr.2021.12505
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Abstract

Intervertebral disc degeneration (IDD) is a major cause of lower back pain. The high morbidity associated with this disease diminishes the quality of life of those who are affected. MicroRNAs (miRs) play crucial roles in various diseases, including IDD. However, the mechanism via which miR‑200c‑3p plays a role in the development of IDD remains unknown. The present study aimed to investigate the effect of miR‑200c‑3p on the progression of IDD and the underlying mechanism. The expression level of miR‑200c‑3p was evaluated in intervertebral disc tissues from patients with IDD. To construct the IDD cell model, the nucleus pulposus (NP) cells were treated with lipopolysaccharide (LPS) 24 h following transfection with miR‑200c‑3p mimic or inhibitor. A luciferase activity assay was performed, while reverse transcription‑quantitative PCR and western blotting were conducted to determine the RNA and protein expression levels, respectively. The expression level of miR‑200c‑3p in the intervertebral disc tissues of patients with IDD was lower than that of normal subjects. LPS treatment reduced the expression level of miR‑200c‑3p in NP cells. Moreover, miR‑200c‑3p mimic inhibited LPS‑induced NP cell apoptosis. It was found that miR‑200c‑3p attenuated inflammatory cytokine levels and extracellular matrix (ECM) degradation in NP cells. Furthermore, miR‑200c‑3p targeted Ras‑related protein 2C (RAP2C) in NP cells. RAP2C promoted apoptosis, inflammatory cytokine levels and ECM degradation by activating ERK signaling. Knockdown of RAP2C and inhibition of ERK signaling by SCH772984 partially reversed the proinflammatory effect of the miR‑200c‑3p inhibitor on LPS‑treated NP cells. Thus, miR‑200c‑3p inhibits NP cell apoptosis, inflammatory cytokine levels and ECM degradation in IDD by targeting RAP2C/ERK signaling.
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Copy and paste a formatted citation
Spandidos Publications style
Cao J, Jiang M, Ren H and Xu K: MicroRNA‑200c‑3p suppresses intervertebral disc degeneration by targeting RAP2C/ERK signaling. Mol Med Rep 24: 865, 2021.
APA
Cao, J., Jiang, M., Ren, H., & Xu, K. (2021). MicroRNA‑200c‑3p suppresses intervertebral disc degeneration by targeting RAP2C/ERK signaling. Molecular Medicine Reports, 24, 865. https://doi.org/10.3892/mmr.2021.12505
MLA
Cao, J., Jiang, M., Ren, H., Xu, K."MicroRNA‑200c‑3p suppresses intervertebral disc degeneration by targeting RAP2C/ERK signaling". Molecular Medicine Reports 24.6 (2021): 865.
Chicago
Cao, J., Jiang, M., Ren, H., Xu, K."MicroRNA‑200c‑3p suppresses intervertebral disc degeneration by targeting RAP2C/ERK signaling". Molecular Medicine Reports 24, no. 6 (2021): 865. https://doi.org/10.3892/mmr.2021.12505
Copy and paste a formatted citation
x
Spandidos Publications style
Cao J, Jiang M, Ren H and Xu K: MicroRNA‑200c‑3p suppresses intervertebral disc degeneration by targeting RAP2C/ERK signaling. Mol Med Rep 24: 865, 2021.
APA
Cao, J., Jiang, M., Ren, H., & Xu, K. (2021). MicroRNA‑200c‑3p suppresses intervertebral disc degeneration by targeting RAP2C/ERK signaling. Molecular Medicine Reports, 24, 865. https://doi.org/10.3892/mmr.2021.12505
MLA
Cao, J., Jiang, M., Ren, H., Xu, K."MicroRNA‑200c‑3p suppresses intervertebral disc degeneration by targeting RAP2C/ERK signaling". Molecular Medicine Reports 24.6 (2021): 865.
Chicago
Cao, J., Jiang, M., Ren, H., Xu, K."MicroRNA‑200c‑3p suppresses intervertebral disc degeneration by targeting RAP2C/ERK signaling". Molecular Medicine Reports 24, no. 6 (2021): 865. https://doi.org/10.3892/mmr.2021.12505
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