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Long non‑coding RNA CTBP1‑AS2 upregulates USP22 to promote pancreatic carcinoma progression by sponging miR‑141‑3p

  • Authors:
    • Mingliang Zhang
    • Songbo Ma
    • Xuzhao Li
    • Henghai Yu
    • Yizheng Tan
    • Jun He
    • Xiaoping Wei
    • Junming Ma
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, People's Hospital of Ningxia Hui Autonomous Region, Yinchuan, Ningxia Hui Autonomous Region 750021, P.R. China, Department of Hepatopancreatobiliary Surgery, The 2nd Hospital of Kunming Medical University, Kunming, Yunnan 650101, P.R. China
    Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY 4.0].
  • Article Number: 86
    |
    Published online on: January 14, 2022
       https://doi.org/10.3892/mmr.2022.12602
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Abstract

Long non‑coding RNAs (lncRNAs) feature prominently in pancreatic carcinoma progression. The present study aimed to clarify the biological functions, clinical significance and underlying mechanism of lncRNA CTBP1 antisense RNA 2 (CTBP1‑AS2) in pancreatic carcinoma. Reverse transcription‑quantitative PCR was performed to assess the expression levels of CTBP1‑AS2, microRNA (miR)‑141‑3p and ubiquitin‑specific protease 22 (USP22) mRNA in pancreatic carcinoma tissues and cell lines. Western blotting was used to examine USP22 protein expression in pancreatic carcinoma cell lines. Loss‑of‑function experiments were used to analyze the regulatory effects of CTBP1‑AS2 on proliferation, apoptosis, migration and invasion of pancreatic carcinoma cells. Dual‑luciferase reporter assay was used to examine the binding relationship between CTBP1‑AS2 and miR‑141‑3p, as well as between miR‑141‑3p and USP22. It was demonstrated that CTBP1‑AS2 expression was markedly increased in pancreatic carcinoma tissues and cell lines. High CTBP1‑AS2 expression was associated with advanced clinical stage and lymph node metastasis of patients. Functional experiments confirmed that knocking down CTBP1‑AS2 significantly inhibited pancreatic carcinoma cell proliferation, migration and invasion, and promoted cell apoptosis. In terms of mechanism, it was found that CTBP1‑AS2 adsorbed miR‑141‑3p as a molecular sponge to upregulate the expression level of USP22. In conclusion, lncRNA CTBP1‑AS2 may be involved in pancreatic carcinoma progression by regulating miR‑141‑3p and USP22 expressions; in addition, CTBP1‑AS2 may be a diagnostic biomarker and treatment target for pancreatic carcinoma.
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1 

Grossberg AJ, Chu LC, Deig CR, Fishman EK, Hwang WL, Maitra A, Marks DL, Mehta A, Nabavizadeh N, Simeone DM, et al: Multidisciplinary standards of care and recent progress in pancreatic ductal adenocarcinoma. CA Cancer J Clin. 70:375–403. 2020. View Article : Google Scholar : PubMed/NCBI

2 

Wang P, Wang J, Tan H, Weng S, Cheng L, Zhou Z and Wen S: Acid- and reduction-sensitive micelles for improving the drug delivery efficacy for pancreatic cancer therapy. Biomater Sci. 6:1262–1270. 2018. View Article : Google Scholar : PubMed/NCBI

3 

Sheng J, Wang L, Han Y, Chen W, Liu H, Zhang M, Deng L and Liu YN: Dual roles of protein as a template and a sulfur provider: A general approach to metal sulfides for efficient photothermal therapy of cancer. Small. 14:Nov 17–2017.doi: 10.1002/smll.201702529. PubMed/NCBI

4 

Batista IA and Melo SA: Exosomes and the future of immunotherapy in pancreatic cancer. Int J Mol Sci. 20:5672019. View Article : Google Scholar : PubMed/NCBI

5 

Chan JJ and Tay Y: Noncoding RNA: RNA regulatory networks in cancer. Int J Mol Sci. 19:13102018. View Article : Google Scholar : PubMed/NCBI

6 

Xu T, Lei T, Li SQ, Mai EH, Ding FH and Niu B: DNAH17-AS1 promotes pancreatic carcinoma by increasing PPME1 expression via inhibition of miR-432-5p. World J Gastroenterol. 26:1745–1757. 2020. View Article : Google Scholar : PubMed/NCBI

7 

Zhang H, Feng X, Zhang M, Liu A, Tian L, Bo W, Wang H and Hu Y: Long non-coding RNA CASC2 upregulates PTEN to suppress pancreatic carcinoma cell metastasis by downregulating miR-21. Cancer Cell Int. 19:182019. View Article : Google Scholar : PubMed/NCBI

8 

Guo JQ, Yang ZJ, Wang S, Wu ZZ, Yin LL and Wang DC: LncRNA SNHG16 functions as an oncogene by sponging miR-200a-3p in pancreatic cancer. Eur Rev Med Pharmacol Sci. 24:72182020.PubMed/NCBI

9 

Wang M and Zhao H: LncRNA CTBP1-AS2 promotes cell proliferation in hepatocellular carcinoma by regulating miR-623/Cyclin d1 axis. Cancer Biother Radiopharm. 35:765–770. 2020. View Article : Google Scholar : PubMed/NCBI

10 

Wang QA, Yang Y and Liang X: LncRNA CTBP1-AS2 sponges miR-216a to upregulate PTEN and suppress endometrial cancer cell invasion and migration. J Ovarian Res. 13:372020. View Article : Google Scholar : PubMed/NCBI

11 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) Method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

12 

Tang M, Xie X, Shi M, Xin W, Zheng G, Zhang Y, Zhang Z and Lian X: Antileukemic effect of caffeic acid 3,4-dihydroxyphenetyl ester. Evidences for its mechanisms of action. Phytomedicine. 80:1533832021. View Article : Google Scholar : PubMed/NCBI

13 

Salmena L, Poliseno L, Tay Y, Kats L and Pandolfi PP: A ceRNA hypothesis: The Rosetta Stone of a hidden RNA language? Cell. 146:353–358. 2011. View Article : Google Scholar : PubMed/NCBI

14 

Ning Z, Wang A, Liang J, Xie Y, Liu J, Yan Q and Wang Z: USP22 promotes epithelial-mesenchymal transition via the FAK pathway in pancreatic cancer cells. Oncol Rep. 32:1451–1458. 2014. View Article : Google Scholar : PubMed/NCBI

15 

Mattiuzzi C and Lippi G: Cancer statistics: A comparison between World Health Organization (WHO) and global burden of disease (GBD). Eur J Public Health. 30:1026–1027. 2020. View Article : Google Scholar : PubMed/NCBI

16 

Weng W, Zhang Z, Huang W, Xu X, Wu B, Ye T, Shan Y, Shi K and Lin Z: Identification of a competing endogenous RNA network associated with prognosis of pancreatic adenocarcinoma. Cancer Cell Int. 20:2312020. View Article : Google Scholar : PubMed/NCBI

17 

Yang Y, Gao M, Li Y, Li M and Ma Q: LncRNA CTBP1-AS2 facilitates gastric cancer progression via regulating the miR-139-3p/MMP11 axis. Onco Targets Ther. 13:11537–11547. 2020. View Article : Google Scholar : PubMed/NCBI

18 

Zhang H, Li J, Shao W and Shen N: LncRNA CTBP1-AS2 is upregulated in osteoarthritis and increases the methylation of miR-130a gene to inhibit chondrocyte proliferation. Clin Rheumatol. 39:3473–3478. 2020. View Article : Google Scholar : PubMed/NCBI

19 

Li Y, Zong J and Zhao C: lncRNA CTBP1-AS2 promotes proliferation and migration of glioma by modulating miR-370-3p-Wnt7a-mediated epithelial-mesenchymal transition. Biochem Cell Biol. 98:661–668. 2020. View Article : Google Scholar : PubMed/NCBI

20 

Cui K and Zhu G: LncRNA CTBP1-AS2 regulates miR-216a/PTEN to suppress ovarian cancer cell proliferation. J Ovarian Res. 13:842020. View Article : Google Scholar : PubMed/NCBI

21 

Yang S, Shi F, Du Y, Wang Z, Feng Y, Song J, Liu Y and Xiao M: Long non-coding RNA CTBP1-AS2 enhances cervical cancer progression via up-regulation of ZNF217 through sponging miR-3163. Cancer Cell Int. 20:3432020. View Article : Google Scholar : PubMed/NCBI

22 

Wang L: MiR-141-3p overexpression suppresses the malignancy of osteosarcoma by targeting FUS to degrade LDHB. Biosci Rep. 40:BSR201934042020. View Article : Google Scholar : PubMed/NCBI

23 

Liang Z, Li X, Liu S, Li C, Wang X and Xing J: MiR-141-3p inhibits cell proliferation, migration and invasion by targeting TRAF5 in colorectal cancer. Biochem Biophys Res Commun. 514:699–705. 2019. View Article : Google Scholar : PubMed/NCBI

24 

Sun J and Zhang Y: LncRNA XIST enhanced TGF-β2 expression by targeting miR-141-3p to promote pancreatic cancer cells invasion. Biosci Rep. 39:BSR201903322019. View Article : Google Scholar : PubMed/NCBI

25 

Zhao G, Wang B, Liu Y, Zhang JG, Deng SC, Qin Q, Tian K, Li X, Zhu S, Niu Y, et al: MiRNA-141, downregulated in pancreatic cancer, inhibits cell proliferation and invasion by directly targeting MAP4K4. Mol Cancer Ther. 12:2569–2580. 2013. View Article : Google Scholar : PubMed/NCBI

26 

Melo-Cardenas J, Zhang Y, Zhang DD and Fang D: Ubiquitin-specific peptidase 22 functions and its involvement in disease. Oncotarget. 7:44848–44856. 2016. View Article : Google Scholar : PubMed/NCBI

27 

Lim C, Xu JC, Chen TY, Xu JX, Chen WF, Hu JW, Li QL and Zhang YQ: Ubiquitin-specific peptide 22 acts as an oncogene in gastric cancer in a son of sevenless 1-dependent manner. Cancer Cell Int. 20:452020. View Article : Google Scholar : PubMed/NCBI

28 

Zhou D, Liu P, Sun DW, Chen ZJ, Hu J, Peng SM and Liu YL: USP22 down-regulation facilitates human retinoblastoma cell aging and apoptosis via inhibiting TERT/P53 pathway. Eur Rev Med Pharmacol Sci. 21:2785–2792. 2017.PubMed/NCBI

29 

Li J, Yuan S, Norgard RJ, Yan F, Yamazoe T, Blanco A and Stanger BZ: Tumor Cell-Intrinsic USP22 suppresses antitumor immunity in pancreatic cancer. Cancer Immunol Res. 8:282–291. 2020. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Zhang M, Ma S, Li X, Yu H, Tan Y, He J, Wei X and Ma J: Long non‑coding RNA CTBP1‑AS2 upregulates USP22 to promote pancreatic carcinoma progression by sponging miR‑141‑3p. Mol Med Rep 25: 86, 2022.
APA
Zhang, M., Ma, S., Li, X., Yu, H., Tan, Y., He, J. ... Ma, J. (2022). Long non‑coding RNA CTBP1‑AS2 upregulates USP22 to promote pancreatic carcinoma progression by sponging miR‑141‑3p. Molecular Medicine Reports, 25, 86. https://doi.org/10.3892/mmr.2022.12602
MLA
Zhang, M., Ma, S., Li, X., Yu, H., Tan, Y., He, J., Wei, X., Ma, J."Long non‑coding RNA CTBP1‑AS2 upregulates USP22 to promote pancreatic carcinoma progression by sponging miR‑141‑3p". Molecular Medicine Reports 25.3 (2022): 86.
Chicago
Zhang, M., Ma, S., Li, X., Yu, H., Tan, Y., He, J., Wei, X., Ma, J."Long non‑coding RNA CTBP1‑AS2 upregulates USP22 to promote pancreatic carcinoma progression by sponging miR‑141‑3p". Molecular Medicine Reports 25, no. 3 (2022): 86. https://doi.org/10.3892/mmr.2022.12602
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang M, Ma S, Li X, Yu H, Tan Y, He J, Wei X and Ma J: Long non‑coding RNA CTBP1‑AS2 upregulates USP22 to promote pancreatic carcinoma progression by sponging miR‑141‑3p. Mol Med Rep 25: 86, 2022.
APA
Zhang, M., Ma, S., Li, X., Yu, H., Tan, Y., He, J. ... Ma, J. (2022). Long non‑coding RNA CTBP1‑AS2 upregulates USP22 to promote pancreatic carcinoma progression by sponging miR‑141‑3p. Molecular Medicine Reports, 25, 86. https://doi.org/10.3892/mmr.2022.12602
MLA
Zhang, M., Ma, S., Li, X., Yu, H., Tan, Y., He, J., Wei, X., Ma, J."Long non‑coding RNA CTBP1‑AS2 upregulates USP22 to promote pancreatic carcinoma progression by sponging miR‑141‑3p". Molecular Medicine Reports 25.3 (2022): 86.
Chicago
Zhang, M., Ma, S., Li, X., Yu, H., Tan, Y., He, J., Wei, X., Ma, J."Long non‑coding RNA CTBP1‑AS2 upregulates USP22 to promote pancreatic carcinoma progression by sponging miR‑141‑3p". Molecular Medicine Reports 25, no. 3 (2022): 86. https://doi.org/10.3892/mmr.2022.12602
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