Regulation of dendrite growth by Cdc42 effector protein‑4 in hippocampal neurons <em>in vitro</em>

Hou,Lei; Wang,Lufeng; Zhao,Zhijie; Xu,Wei; Wang,Yang; Cui,Gang

doi:10.3892/mmr.2022.12644

Regulation of dendrite growth by Cdc42 effector protein‑4 in hippocampal neurons in vitro

Authors:
- Lei Hou
- Lufeng Wang
- Zhijie Zhao
- Wei Xu
- Yang Wang
- Gang Cui
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Affiliations: Department of Neurosurgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, P.R. China, Department of Neurology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 200120, P.R. China, Department of Neurosurgery, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200336, P.R. China, Department of Neurosurgery, Huashan Hospital, Fudan University, Shanghai 200040, P.R. China, Department of Emergency, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Yangpu, Shanghai 200092, P.R. China
Published online on: February 15, 2022 https://doi.org/10.3892/mmr.2022.12644
Article Number: 128
Copyright: © Hou et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Cell division control protein 42 homolog (Cdc42), one of the most characteristic members of the Rho protein family, is required for multiple aspects of dendritic morphogenesis. However, the proteins mediating the regulatory effects of Cdc42 activity on neuronal morphology are largely unknown. Cdc42 effector protein‑4 (CEP4) was identified to be a binding partner of Rho GTPase 4 and is ubiquitously expressed in all adult tissues. However, the physiological function of CEP4 in neurons is unknown. In the present study, immunofluorescence and western blot analysis were conducted, revealing that CEP4 is highly expressed in the brain, and that the expression of CEP4 is gradually increased during neurodevelopment. Knockdown of CEP4 with short hairpin RNA suppressed dendrite growth, whereas overexpression of wild‑type CEP4 promoted dendrite growth in primary isolated mouse hippocampal neurons. Collectively, these results indicated an important role for CEP4 in dendrite growth in hippocampal neurons.

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