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miR‑374c‑5p regulates PTTG1 and inhibits cell growth and metastasis in hepatocellular carcinoma by regulating epithelial‑mesenchymal transition

  • Authors:
    • Gang Yang
    • Yongfu Xiog
    • Guan Wang
    • Weinan Li
    • Tao Tang
    • Ji Sun
    • Jingdong Li
  • View Affiliations / Copyright

    Affiliations: First Clinical Medical College, Jinan University, Tianhe, Guangzhou, Guangdong 510632, P.R. China, Department of Hepatobiliary Surgery, Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan 637000, P.R. China, Physical Examination Center, Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan 637000, P.R. China
    Copyright: © Yang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 148
    |
    Published online on: March 1, 2022
       https://doi.org/10.3892/mmr.2022.12664
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Abstract

A large number of studies have reported that microRNA (miR)‑374c‑5p plays an important role in the occurrence and development of malignant tumors, but there is no research on the role of miR‑374c‑5p in hepatocellular carcinoma (HCC). The aim of the present study was to investigate the role of miR‑374c‑5p in HCC and the underlying molecular mechanism. The expression of miR‑374c‑5p in HCC tissues and HCC cell lines was analyzed via reverse transcription‑quantitative PCR. The association between miR‑374c‑5p and clinical pathology was also analyzed in patients with HCC. Kaplan‑Meier analysis and Cox multivariate analysis were used to evaluate the prognostic significance of miR‑374c‑5p in HCC. The biological functions of miR‑374c‑5p, including cell proliferation, migration and invasion and its potential molecular mechanism were analyzed in vivo and in vitro. In addition, the molecular mechanism of miR‑374c‑5p in HCC was further explored. The results demonstrated that miR‑374c‑5p expression was lower in HCC than in matched adjacent tissue samples. Patients with low expression of miR‑374c‑5p had poor prognosis and short survival time. Overexpression of miR‑374c‑5p inhibited HCC cell proliferation, migration and invasion in vitro. In vivo, it was found that overexpression of miR‑374c‑5p significantly inhibited the growth and proliferation of HCC cells. Dual‑luciferase reporter assays verified that miR‑374c‑5p directly targets the 3'‑untranslated region of pituitary tumor‑transforming 1 (PTTG1) and regulates PTTG1 expression. In general, it was revealed that miR‑374c‑5p regulates the malignant biological behavior of HCC through PTTG1, thereby affecting epithelial‑mesenchymal transition. Thus, miR‑374c‑5p is a potential biological indicator to predict poor prognosis in patients with HCC.
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Copy and paste a formatted citation
Spandidos Publications style
Yang G, Xiog Y, Wang G, Li W, Tang T, Sun J and Li J: miR‑374c‑5p regulates PTTG1 and inhibits cell growth and metastasis in hepatocellular carcinoma by regulating epithelial‑mesenchymal transition. Mol Med Rep 25: 148, 2022.
APA
Yang, G., Xiog, Y., Wang, G., Li, W., Tang, T., Sun, J., & Li, J. (2022). miR‑374c‑5p regulates PTTG1 and inhibits cell growth and metastasis in hepatocellular carcinoma by regulating epithelial‑mesenchymal transition. Molecular Medicine Reports, 25, 148. https://doi.org/10.3892/mmr.2022.12664
MLA
Yang, G., Xiog, Y., Wang, G., Li, W., Tang, T., Sun, J., Li, J."miR‑374c‑5p regulates PTTG1 and inhibits cell growth and metastasis in hepatocellular carcinoma by regulating epithelial‑mesenchymal transition". Molecular Medicine Reports 25.4 (2022): 148.
Chicago
Yang, G., Xiog, Y., Wang, G., Li, W., Tang, T., Sun, J., Li, J."miR‑374c‑5p regulates PTTG1 and inhibits cell growth and metastasis in hepatocellular carcinoma by regulating epithelial‑mesenchymal transition". Molecular Medicine Reports 25, no. 4 (2022): 148. https://doi.org/10.3892/mmr.2022.12664
Copy and paste a formatted citation
x
Spandidos Publications style
Yang G, Xiog Y, Wang G, Li W, Tang T, Sun J and Li J: miR‑374c‑5p regulates PTTG1 and inhibits cell growth and metastasis in hepatocellular carcinoma by regulating epithelial‑mesenchymal transition. Mol Med Rep 25: 148, 2022.
APA
Yang, G., Xiog, Y., Wang, G., Li, W., Tang, T., Sun, J., & Li, J. (2022). miR‑374c‑5p regulates PTTG1 and inhibits cell growth and metastasis in hepatocellular carcinoma by regulating epithelial‑mesenchymal transition. Molecular Medicine Reports, 25, 148. https://doi.org/10.3892/mmr.2022.12664
MLA
Yang, G., Xiog, Y., Wang, G., Li, W., Tang, T., Sun, J., Li, J."miR‑374c‑5p regulates PTTG1 and inhibits cell growth and metastasis in hepatocellular carcinoma by regulating epithelial‑mesenchymal transition". Molecular Medicine Reports 25.4 (2022): 148.
Chicago
Yang, G., Xiog, Y., Wang, G., Li, W., Tang, T., Sun, J., Li, J."miR‑374c‑5p regulates PTTG1 and inhibits cell growth and metastasis in hepatocellular carcinoma by regulating epithelial‑mesenchymal transition". Molecular Medicine Reports 25, no. 4 (2022): 148. https://doi.org/10.3892/mmr.2022.12664
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