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E. coli JM83 damages the mucosal barrier in Ednrb knockout mice to promote the development of Hirschsprung‑associated enterocolitis via activation of TLR4/p‑p38/NF‑κB signaling

  • Authors:
    • Zebing Zheng
    • Mingjuan Gao
    • Chengyan Tang
    • Lu Huang
    • Yuan Gong
    • Yuanmei Liu
    • Jian Wang
  • View Affiliations / Copyright

    Affiliations: Department of Pediatric Surgery, Children's Hospital of Soochow University, Pediatric Research Institute of Soochow University, Suzhou, Jiangsu 215123, P.R. China, Department of Pediatric Surgery, Affiliated Hospital of Zunyi Medical University, Zunyi, Guizhou 563000, P.R. China, Department of Pediatric Surgery, Affiliated Hospital of Zunyi Medical University, Zunyi, Guizhou 563000, P.R. China, Department of Pediatric Surgery, Children's Hospital of Soochow University, Pediatric Research Institute of Soochow University, Suzhou, Jiangsu 215123, P.R. China
    Copyright: © Zheng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 168
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    Published online on: March 16, 2022
       https://doi.org/10.3892/mmr.2022.12684
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Abstract

Hirschsprung‑associated enterocolitis (HAEC) is characterized by intestinal mucosal damage and an imbalance in the intestinal microbiota. Recent studies have indicated that the TLR4/p‑p38/NF‑κB signaling pathway in the intestine is of great importance to intestinal mucosal integrity. The present study aimed to investigate the role of TLR4/phosphorylated (p‑)38/NF‑κB signaling in the pathogenesis of HAEC in E. coli JM83‑infected endothelin receptor B (Ednrb)‑/‑ mice. Ednrb‑/‑ mice were infected with E. coli JM83 by oral gavage to establish the HAEC model. Wild‑type and Ednrb‑/‑ mice were randomly divided into uninfected and E. coli groups. The role of TLR4/p‑p38/NF‑κB signaling was further evaluated by in vivo and in vitro analyses. The activation of the TLR4/p‑p38/NF‑κB signaling pathway induced by E. coli JM83 resulted in HAEC in Ednrb‑/‑ mice, which was evidenced by a significant increase in the expression of TNF‑α, TGF‑β and IL‑10, and a decreased density of F‑actin protein expression. TLR4 knockdown reduced the severity of enterocolitis and attenuated the expression of IL‑10, TNF‑α and TGF‑β, whilst increasing the density of F‑actin protein in Ednrb‑/‑ mice after E. coli infection. These results indicated that E. coli JM83 activates TLR4/p‑p38/NF‑κB signaling in Ednrb‑/‑ to promote the development of HAEC. Thus, inhibition of this signaling pathway may benefit the treatment and prevention of HAEC.
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Copy and paste a formatted citation
Spandidos Publications style
Zheng Z, Gao M, Tang C, Huang L, Gong Y, Liu Y and Wang J: <em>E. coli</em> JM83 damages the mucosal barrier in Ednrb knockout mice to promote the development of Hirschsprung‑associated enterocolitis via activation of TLR4/p‑p38/NF‑κB signaling. Mol Med Rep 25: 168, 2022.
APA
Zheng, Z., Gao, M., Tang, C., Huang, L., Gong, Y., Liu, Y., & Wang, J. (2022). <em>E. coli</em> JM83 damages the mucosal barrier in Ednrb knockout mice to promote the development of Hirschsprung‑associated enterocolitis via activation of TLR4/p‑p38/NF‑κB signaling. Molecular Medicine Reports, 25, 168. https://doi.org/10.3892/mmr.2022.12684
MLA
Zheng, Z., Gao, M., Tang, C., Huang, L., Gong, Y., Liu, Y., Wang, J."<em>E. coli</em> JM83 damages the mucosal barrier in Ednrb knockout mice to promote the development of Hirschsprung‑associated enterocolitis via activation of TLR4/p‑p38/NF‑κB signaling". Molecular Medicine Reports 25.5 (2022): 168.
Chicago
Zheng, Z., Gao, M., Tang, C., Huang, L., Gong, Y., Liu, Y., Wang, J."<em>E. coli</em> JM83 damages the mucosal barrier in Ednrb knockout mice to promote the development of Hirschsprung‑associated enterocolitis via activation of TLR4/p‑p38/NF‑κB signaling". Molecular Medicine Reports 25, no. 5 (2022): 168. https://doi.org/10.3892/mmr.2022.12684
Copy and paste a formatted citation
x
Spandidos Publications style
Zheng Z, Gao M, Tang C, Huang L, Gong Y, Liu Y and Wang J: <em>E. coli</em> JM83 damages the mucosal barrier in Ednrb knockout mice to promote the development of Hirschsprung‑associated enterocolitis via activation of TLR4/p‑p38/NF‑κB signaling. Mol Med Rep 25: 168, 2022.
APA
Zheng, Z., Gao, M., Tang, C., Huang, L., Gong, Y., Liu, Y., & Wang, J. (2022). <em>E. coli</em> JM83 damages the mucosal barrier in Ednrb knockout mice to promote the development of Hirschsprung‑associated enterocolitis via activation of TLR4/p‑p38/NF‑κB signaling. Molecular Medicine Reports, 25, 168. https://doi.org/10.3892/mmr.2022.12684
MLA
Zheng, Z., Gao, M., Tang, C., Huang, L., Gong, Y., Liu, Y., Wang, J."<em>E. coli</em> JM83 damages the mucosal barrier in Ednrb knockout mice to promote the development of Hirschsprung‑associated enterocolitis via activation of TLR4/p‑p38/NF‑κB signaling". Molecular Medicine Reports 25.5 (2022): 168.
Chicago
Zheng, Z., Gao, M., Tang, C., Huang, L., Gong, Y., Liu, Y., Wang, J."<em>E. coli</em> JM83 damages the mucosal barrier in Ednrb knockout mice to promote the development of Hirschsprung‑associated enterocolitis via activation of TLR4/p‑p38/NF‑κB signaling". Molecular Medicine Reports 25, no. 5 (2022): 168. https://doi.org/10.3892/mmr.2022.12684
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