Protective effect of 17S‑epoxy‑docosapentaenoic acid against dextran sulfate sodium induced ulcerative colitis in BALB/c mice.
- Lifang Wang
- Hack Sun Choi
- Yan Su
- Binna Lee
- Jong Hyun Choi
- Sun-Hee Jang
- Yong-Suk Jang
- Jeong-Woo Seo
Affiliations: Korea Research Institute of Bioscience and Biotechnology, Microbial Biotechnology Research Center, Jeongeup, Jeollabuk‑do 56212, Republic of Korea, College of Applied Life Sciences, Jeju National University, Jeju 63243, Republic of Korea, Korea Research Institute of Bioscience and Biotechnology, Microbial Biotechnology Research Center, Jeongeup, Jeollabuk‑do 56212, Republic of Korea, Department of Bioactive Material Sciences, The Institute for Molecular Biology and Genetics, Jeonbuk National University, Jeonju, Jeollabuk‑do 54896, Republic of Korea
- Published online on: July 15, 2022 https://doi.org/10.3892/mmr.2022.12794
Copyright: © Wang
et al. This is an open access article distributed under the
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Ulcerative colitis (UC) is difficult to eradicate as it leads to chronic inflammation in the digestive tract due to immune system malfunction. The present study demonstrated the protective effect of 7S,15R‑dihydroxy‑16S,17S‑epoxy‑docosapentaenoic acid (diHEP‑DPA), which had been previously synthesized, on a dextran sulfate sodium (DSS)‑induced BALB/c mouse model of UC. UC was induced with 4% DSS drinking water for 7 days. Initially, the anti‑inflammatory effect of diHEP‑DPA was confirmed by demonstrating that lipopolysaccharide‑stimulated THP1 cells treated with diHEP‑DPA decreased IL‑6, TNF‑α and nitrite levels by fluorescence‑activated cell sorting (FACS) and Griess reagent kit. The results indicated that the administration of diHEP‑DPA at 20 µg/kg significantly reduced the severity of colitis, as determined by hematoxylin and eosin staining. The levels of TNF‑α, IL‑6 and IL‑1β in the colon tissue and serum were significantly reduced in the diHEP‑DPA + DSS‑treated group compared with in the control group, as determined by FACS and ELISA kit. It was also observed that diHEP‑DPA decreased myeloperoxidase (MPO) and nitrite levels in the colon tissues of diHEP‑DPA + DSS‑treated mice, as indicated using commercial MPO and nitric oxide kits. The diHEP‑DPA+DSS‑treated mice also exhibited decreased expression levels of phosporylated (p)‑inhibitor κB protein, p‑p65 and inducible nitric oxide synthase in the colon tissue by inhibiting inflammation, which were measured by reverse transcription‑quantitative PCR and weatern blot analysis. Overall, the present study demonstrated the protective effect of diHEP‑DPA against a severe colitis condition in vivo.