Open Access

Macrophage migration inhibitory factor takes part in the lumbar ligamentum flavum hypertrophy

  • Authors:
    • Qi-Lin Lu
    • Zi-Xuan Zheng
    • Yu-Hui Ye
    • Jiang-Yun Lu
    • Yu-Qi Zhong
    • Chao Sun
    • Cheng-Jie Xiong
    • Gong-Xu Yang
    • Feng Xu
  • View Affiliations

  • Published online on: July 27, 2022     https://doi.org/10.3892/mmr.2022.12805
  • Article Number: 289
  • Copyright: © Lu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study aimed to observe the content difference of macrophage migration inhibitory factor [MIF; novoprotein recombinant human MIF (n‑6his) (ch33)], TGFβ1 and MMP13 in patients with and without ligamentum flavum (LF) hypertrophy and investigate the roles of MIF in LF hypertrophy. The concentration of MIF, TGFβ1 and MMP13 in LF were detected by ELISA in a lumbar spinal stenosis (LSS) group and a lumbar disc herniation (LDH) group. Culture of primary LFs and identification were performed for the subsequent study. Cell treatments and cell proliferation assay by CCK‑8 was performed. Western blot and quantitative PCR analysis were used to detect the expression of TGFβ1, MMP13, type I collagen (COL‑1) and type III collagen (COL‑3) and Src which were promoted by MIF. The concentration of MIF, TGFβ1 and MMP13 were higher in the LSS group compared with the LDH group. Culture of primary LFs and identification were performed. Significant difference in LFs proliferation occurred with treatment by MIF at a concentration of 40 nM for 48 h (P<0.05). The gene and protein expression of TGFβ1, MMP13, COL‑1, COL‑3 and Src were promoted by MIF (P<0.05). Proliferation of LFs was induced by MIF and MIF‑induced proliferation of LFs was inhibited by PP1 (a Src inhibitor). MIF may promote the proliferation of LFs through the Src kinase signaling pathway and can promote extracellular matrix changes by its pro‑inflammatory effect. MIF and its mediated inflammatory reaction are driving factors of LF hypertrophy.
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September-2022
Volume 26 Issue 3

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Copy and paste a formatted citation
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Spandidos Publications style
Lu Q, Zheng Z, Ye Y, Lu J, Zhong Y, Sun C, Xiong C, Yang G and Xu F: Macrophage migration inhibitory factor takes part in the lumbar ligamentum flavum hypertrophy. Mol Med Rep 26: 289, 2022
APA
Lu, Q., Zheng, Z., Ye, Y., Lu, J., Zhong, Y., Sun, C. ... Xu, F. (2022). Macrophage migration inhibitory factor takes part in the lumbar ligamentum flavum hypertrophy. Molecular Medicine Reports, 26, 289. https://doi.org/10.3892/mmr.2022.12805
MLA
Lu, Q., Zheng, Z., Ye, Y., Lu, J., Zhong, Y., Sun, C., Xiong, C., Yang, G., Xu, F."Macrophage migration inhibitory factor takes part in the lumbar ligamentum flavum hypertrophy". Molecular Medicine Reports 26.3 (2022): 289.
Chicago
Lu, Q., Zheng, Z., Ye, Y., Lu, J., Zhong, Y., Sun, C., Xiong, C., Yang, G., Xu, F."Macrophage migration inhibitory factor takes part in the lumbar ligamentum flavum hypertrophy". Molecular Medicine Reports 26, no. 3 (2022): 289. https://doi.org/10.3892/mmr.2022.12805