Oridonin attenuates dextran sulfate sodium‑induced ulcerative colitis in mice via the Sirt1/NF‑κB/p53 pathway

Wang,Maonan; Xu,Bo; Liu,Lintao; Wang,Dawei

doi:10.3892/mmr.2022.12828

Oridonin attenuates dextran sulfate sodium‑induced ulcerative colitis in mice via the Sirt1/NF‑κB/p53 pathway

Authors:
- Maonan Wang
- Bo Xu
- Lintao Liu
- Dawei Wang
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Affiliations: General Surgery Department, Jilin Provincial People's Hospital, Changchun, Jilin 130021, P.R. China, Faculty of Medicine, Beihua University, Jilin, Jilin 132013, P.R. China
Published online on: August 17, 2022 https://doi.org/10.3892/mmr.2022.12828
Article Number: 312
Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Ulcerative colitis (UC) is a serious chronic inflammatory bowel disease. Oridonin (Ori) has anti‑inflammatory, antibacterial and antitumor activities. The current study aimed to investigate the regulatory role of Ori in UC. BALB/C mice were induced to form a model of UC using dextran sulfate sodium (DSS), after which UC mice received high‑(Ori‑H) and low‑doses of Ori (Ori‑L). Subsequently, the length of the colon was measured and hematoxylin and, eosin staining was performed to detect colonic injury. Western blot analysis was performed to detect expression level in tight junction‑associated proteins in murine colon tissue. Additionally, myeloperoxidase activity and inflammatory factor concentration were detected in colon tissue using ELISA. TUNEL and western blot assays were also performed to detect cell apoptosis, and the expression level of Sirt1/NF‑κB/p53 pathway‑related proteins was also determined using western blot analysis. The results revealed that Ori ameliorated clinical symptoms and pathological lesions in mice with DSS‑induced UC. Furthermore, Ori protected the integrity of the colonic mucosal barrier, reduced the inflammatory response and decreased oxidative stress levels in mice with DSS‑induced UC. Ori treatment also inhibited intestinal mucosal cell apoptosis. These effects may have occurred via the Sirtuin‑1/NF‑κB/p53 pathway. In conclusion, Ori treatment inhibited DSS‑induced inflammatory response, oxidative stress and intestinal mucosal apoptosis in UC mice.

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