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DCXR promotes cell proliferation by promoting the activity of aerobic glycolysis in breast cancer

  • Authors:
    • Yongmei Jin
    • Miao Zhang
    • Yang Tong
    • Lin Qiu
    • Ying Ye
    • Bin Zhao
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, The Seventh People's Hospital of Shanghai University of Traditional Chinese Medicine, Shanghai 200137, P.R. China, Central Laboratory, The Seventh People's Hospital of Shanghai University of Traditional Chinese Medicine, Shanghai 200137, P.R. China
    Copyright: © Jin et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 31
    |
    Published online on: December 16, 2022
       https://doi.org/10.3892/mmr.2022.12918
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Abstract

The function of human dicarbonyl/L‑xylulose reductase (DCXR) in the pathophysiology of breast cancer is yet to be elucidated. The present study aimed to investigate the function of DCXR in glycolysis and the cell cycle of breast cancer cells with respect to cell proliferation. Differential expressed DCXR was identified in The Cancer Genome Atlas (TCGA) database and verified in clinical breast cancer tissue. DCXR silencing and overexpression were induced by RNA interference and lentiviral vectors, respectively. Cell cycle progression, proliferation and glycolytic activity of breast cancer cells were detected by flow cytometry, Cell Counting Kit‑8 assay and chemical methods, respectively. Tumorigenicity was detected using nude mice xenograft models. The expression of DCXR was increased in TCGA breast cancer database and the function of DCXR was enriched in ‘glycolysis’ and ‘cell cycle’. Further analysis using clinical breast cancer samples confirmed upregulation of DCXR. The silencing of DCXR suppressed proliferation and cell cycle progression of breast cancer cells and significantly decreased the capacity for glycolysis, thereby demonstrating the effect of DCXR on the function of breast cancer cells. Similar conclusions were obtained in DCXR overexpressing cells; notably, DCXR overexpression promoted proliferation, cell cycle progression at S phase and glycolysis. 2‑Deoxy‑D‑glucose inhibited the effect of DCXR on the proliferation and cell cycle progression of breast cancer cells. The present study revealed that DCXR regulated breast cancer cell cycle progression and proliferation by increasing glycolysis activity and thus may serve as an oncogene for breast cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Jin Y, Zhang M, Tong Y, Qiu L, Ye Y and Zhao B: DCXR promotes cell proliferation by promoting the activity of aerobic glycolysis in breast cancer. Mol Med Rep 27: 31, 2023.
APA
Jin, Y., Zhang, M., Tong, Y., Qiu, L., Ye, Y., & Zhao, B. (2023). DCXR promotes cell proliferation by promoting the activity of aerobic glycolysis in breast cancer. Molecular Medicine Reports, 27, 31. https://doi.org/10.3892/mmr.2022.12918
MLA
Jin, Y., Zhang, M., Tong, Y., Qiu, L., Ye, Y., Zhao, B."DCXR promotes cell proliferation by promoting the activity of aerobic glycolysis in breast cancer". Molecular Medicine Reports 27.2 (2023): 31.
Chicago
Jin, Y., Zhang, M., Tong, Y., Qiu, L., Ye, Y., Zhao, B."DCXR promotes cell proliferation by promoting the activity of aerobic glycolysis in breast cancer". Molecular Medicine Reports 27, no. 2 (2023): 31. https://doi.org/10.3892/mmr.2022.12918
Copy and paste a formatted citation
x
Spandidos Publications style
Jin Y, Zhang M, Tong Y, Qiu L, Ye Y and Zhao B: DCXR promotes cell proliferation by promoting the activity of aerobic glycolysis in breast cancer. Mol Med Rep 27: 31, 2023.
APA
Jin, Y., Zhang, M., Tong, Y., Qiu, L., Ye, Y., & Zhao, B. (2023). DCXR promotes cell proliferation by promoting the activity of aerobic glycolysis in breast cancer. Molecular Medicine Reports, 27, 31. https://doi.org/10.3892/mmr.2022.12918
MLA
Jin, Y., Zhang, M., Tong, Y., Qiu, L., Ye, Y., Zhao, B."DCXR promotes cell proliferation by promoting the activity of aerobic glycolysis in breast cancer". Molecular Medicine Reports 27.2 (2023): 31.
Chicago
Jin, Y., Zhang, M., Tong, Y., Qiu, L., Ye, Y., Zhao, B."DCXR promotes cell proliferation by promoting the activity of aerobic glycolysis in breast cancer". Molecular Medicine Reports 27, no. 2 (2023): 31. https://doi.org/10.3892/mmr.2022.12918
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