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Article Open Access

Sevoflurane: A dual modulator of miR‑211‑5p and mitochondrial apoptosis in glioma therapy

  • Authors:
    • Haili Wang
    • Guofang Cheng
    • Shuyuan Zhang
    • Haibo Qu
    • Xibo Zhao
    • Ailing Yang
    • Xuejia Sun
    • Hua Pan
  • View Affiliations / Copyright

    Affiliations: Department of Anesthesiology, Sanmenxia Central Hospital of Henan University of Science and Technology, Sanmenxia, Henan 472000, P.R. China, Department of Orthopedics, Sanmenxia Orthopedic Hospital, Sanmenxia, Henan 472000, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 179
    |
    Published online on: April 23, 2025
       https://doi.org/10.3892/mmr.2025.13544
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Abstract

The present study aimed to investigate how sevoflurane (SEV) regulated the apoptosis of glioma cells through the mitochondrial apoptosis pathway. First, an evaluation was performed on the viability, apoptosis, mitochondrial reactive oxygen species levels, mitochondrial membrane potential and apoptosis and autophagy‑related protein expression of glioma cells according to experimental groups. Next, the expression of microRNA‑211‑5p (miR‑211‑5p), silent information regulator 1 (SIRT1) and phosphatidylinositol 3‑kinase (PI3K)/protein kinase B (AKT) signaling pathway was detected by reverse transcription‑quantitative PCR or western blotting. Dual luciferase reporter gene assay confirmed the targeting relationship between miR‑211‑5p and SIRT1. In addition, SEV suppressed the proliferation and induced the apoptosis in human glioma cell line cells via the mitochondrial apoptosis pathway. In mechanistic analysis, the miR‑211‑5p level in glioma cells was low, while following SEV treatment, it was increased. Furthermore, SEV regulated SIRT1 by upregulating miR‑211‑5p expression, thereby blocking the PI3K/AKT signaling pathway activation. Moreover, functional rescue experiments showed that downregulation of SIRT1 or miR‑211‑5p could reverse the effects of SEV on glioma cells. Collectively, SEV promoted apoptosis in glioma cells by inducing miR‑211‑5p, which regulated SIRT1/PI3K/AKT pathway, mediating mitochondria‑dependent apoptosis pathway. This finding may open new possibilities for SEV as a potential treatment for glioma in the future.
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Copy and paste a formatted citation
Spandidos Publications style
Wang H, Cheng G, Zhang S, Qu H, Zhao X, Yang A, Sun X and Pan H: Sevoflurane: A dual modulator of miR‑211‑5p and mitochondrial apoptosis in glioma therapy. Mol Med Rep 32: 179, 2025.
APA
Wang, H., Cheng, G., Zhang, S., Qu, H., Zhao, X., Yang, A. ... Pan, H. (2025). Sevoflurane: A dual modulator of miR‑211‑5p and mitochondrial apoptosis in glioma therapy. Molecular Medicine Reports, 32, 179. https://doi.org/10.3892/mmr.2025.13544
MLA
Wang, H., Cheng, G., Zhang, S., Qu, H., Zhao, X., Yang, A., Sun, X., Pan, H."Sevoflurane: A dual modulator of miR‑211‑5p and mitochondrial apoptosis in glioma therapy". Molecular Medicine Reports 32.1 (2025): 179.
Chicago
Wang, H., Cheng, G., Zhang, S., Qu, H., Zhao, X., Yang, A., Sun, X., Pan, H."Sevoflurane: A dual modulator of miR‑211‑5p and mitochondrial apoptosis in glioma therapy". Molecular Medicine Reports 32, no. 1 (2025): 179. https://doi.org/10.3892/mmr.2025.13544
Copy and paste a formatted citation
x
Spandidos Publications style
Wang H, Cheng G, Zhang S, Qu H, Zhao X, Yang A, Sun X and Pan H: Sevoflurane: A dual modulator of miR‑211‑5p and mitochondrial apoptosis in glioma therapy. Mol Med Rep 32: 179, 2025.
APA
Wang, H., Cheng, G., Zhang, S., Qu, H., Zhao, X., Yang, A. ... Pan, H. (2025). Sevoflurane: A dual modulator of miR‑211‑5p and mitochondrial apoptosis in glioma therapy. Molecular Medicine Reports, 32, 179. https://doi.org/10.3892/mmr.2025.13544
MLA
Wang, H., Cheng, G., Zhang, S., Qu, H., Zhao, X., Yang, A., Sun, X., Pan, H."Sevoflurane: A dual modulator of miR‑211‑5p and mitochondrial apoptosis in glioma therapy". Molecular Medicine Reports 32.1 (2025): 179.
Chicago
Wang, H., Cheng, G., Zhang, S., Qu, H., Zhao, X., Yang, A., Sun, X., Pan, H."Sevoflurane: A dual modulator of miR‑211‑5p and mitochondrial apoptosis in glioma therapy". Molecular Medicine Reports 32, no. 1 (2025): 179. https://doi.org/10.3892/mmr.2025.13544
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