Open Access

β‑elemene attenuates IRI‑AKI by inhibiting inflammation and apoptosis via suppression of the TLR4/MyD88/NF‑κB/MAPK signal axis activation

  • Authors:
    • Qiming Gong
    • Yakun Wang
    • Fahui Liu
    • Yuqing Huang
    • Luxin Li
    • Dongsheng Cheng
    • Shoujun Bai
    • Wenjuan Sun
  • View Affiliations

  • Published online on: June 3, 2025     https://doi.org/10.3892/mmr.2025.13586
  • Article Number: 221
  • Copyright: © Gong et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Ischemia‑reperfusion injury‑induced acute kidney injury (IRI‑AKI) involves inflammatory cell infiltration and increased apoptosis, although the potential association between these processes remains unclear. The aim of the present study was to assess the impact of β‑elemene treatment on the IRI‑AKI using both in vivo and in vitro models, reverse transcription‑quantitative PCR, western blot assays, hematoxylin and Eosin (H&E) staining, Immunohistochemical staining and TUNEL staining. β‑elemene significantly decreased morphological and pathological kidney inflammation in mice caused by IRI. Additionally, β‑elemene prevented the expression of inflammatory factors and apoptosis proteins dose‑dependently in IRI mice and rat renal proximal tubule NRK52E cells treated with H2O2. Mechanistically, β‑elemene exerted its effects by inhibiting toll‑like receptor 4/myeloid differentiation primary response gene 88 (MyD88) signal activation and blocking NF‑κB/MAPK signal phosphorylation. Additionally, the increases in apoptosis and MAPK signal activation following hydrogen peroxide treatment were restored by MyD88 inhibition in NRK52E cells. The present study revealed that β‑elemene is a promising preclinical candidate for AKI.
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August-2025
Volume 32 Issue 2

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Gong Q, Wang Y, Liu F, Huang Y, Li L, Cheng D, Bai S and Sun W: β‑elemene attenuates IRI‑AKI by inhibiting inflammation and apoptosis via suppression of the TLR4/MyD88/NF‑κB/MAPK signal axis activation. Mol Med Rep 32: 221, 2025.
APA
Gong, Q., Wang, Y., Liu, F., Huang, Y., Li, L., Cheng, D. ... Sun, W. (2025). β‑elemene attenuates IRI‑AKI by inhibiting inflammation and apoptosis via suppression of the TLR4/MyD88/NF‑κB/MAPK signal axis activation. Molecular Medicine Reports, 32, 221. https://doi.org/10.3892/mmr.2025.13586
MLA
Gong, Q., Wang, Y., Liu, F., Huang, Y., Li, L., Cheng, D., Bai, S., Sun, W."β‑elemene attenuates IRI‑AKI by inhibiting inflammation and apoptosis via suppression of the TLR4/MyD88/NF‑κB/MAPK signal axis activation". Molecular Medicine Reports 32.2 (2025): 221.
Chicago
Gong, Q., Wang, Y., Liu, F., Huang, Y., Li, L., Cheng, D., Bai, S., Sun, W."β‑elemene attenuates IRI‑AKI by inhibiting inflammation and apoptosis via suppression of the TLR4/MyD88/NF‑κB/MAPK signal axis activation". Molecular Medicine Reports 32, no. 2 (2025): 221. https://doi.org/10.3892/mmr.2025.13586