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November-December 2011 Volume 2 Issue 6

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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

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Article

Mutations of the epidermal growth factor receptor gene in NSCLC patients

  • Authors:
    • Bing Han
    • Xiang Zhou
    • Rong-Xin Zhang
    • Wang-Fu Zang
    • Zhong-Yuan Chen
    • Huai-Dong Song
    • Huan-Ying Wan
    • Cui-Xia Zheng
  • View Affiliations / Copyright

    Affiliations: Department of Respiration, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, P.R. China, Department of Thoracic Surgery, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, P.R. China, Department of Oncosurgery, Bengbu Medical College, Bengbu, P.R. China, Center of Molecular Medicine, Shanghai Institute of Endocrinology, State Key Laboratory of Medical Genomics, Shanghai, P.R. China, Department of Respiration, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, P.R. China
  • Pages: 1233-1237
    |
    Published online on: July 25, 2011
       https://doi.org/10.3892/ol.2011.366
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Abstract

Mutations of the epidermal growth factor receptor (EGFR) in patients with non-small cell lung cancer (NSCLC) were identified by re-sequencing all exons of this gene to evaluate the frequencies of EGFR gene mutation and identify rare or novel EGFR mutations. A total of 55 NSCLC samples from 55 patients were included in the study. Genomic DNA was extracted and exons 1-28 of the EGFR gene were sequenced to identify mutations. The cDNA of the EGFR gene with P848L and T790M double mutants was constructed by introducing point mutations into the wild‑type EGFR vector using a site‑directed mutagenesis kit. Among the 55 patients with NSCLC, 8 patients carried mutations of the EGFR gene. Notably, of the mutation‑harboring patients with a pathological type of adenocarcinoma, 6 were non-smokers. The in vitro study demonstrated that the P848L mutant had a similar response to that of the wild‑type EGFR after gefitinib treatment, and the P848L and T790M double mutant exhibited high resistance to gefitinib. These EGFR mutations preferentially occurred in lung adenocarcinoma patients, most of whom were non‑smokers. In the in vitro study, P848L mutant EGFR had a similar response as the wild‑type EGFR to gefitinib treatment, suggesting that lung cancer patients with a rare mutation of EGFR, such as the P848L mutation, do not respond to gefitinib treatment.
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Copy and paste a formatted citation
Spandidos Publications style
Han B, Zhou X, Zhang R, Zang W, Chen Z, Song H, Wan H and Zheng C: Mutations of the epidermal growth factor receptor gene in NSCLC patients. Oncol Lett 2: 1233-1237, 2011.
APA
Han, B., Zhou, X., Zhang, R., Zang, W., Chen, Z., Song, H. ... Zheng, C. (2011). Mutations of the epidermal growth factor receptor gene in NSCLC patients. Oncology Letters, 2, 1233-1237. https://doi.org/10.3892/ol.2011.366
MLA
Han, B., Zhou, X., Zhang, R., Zang, W., Chen, Z., Song, H., Wan, H., Zheng, C."Mutations of the epidermal growth factor receptor gene in NSCLC patients". Oncology Letters 2.6 (2011): 1233-1237.
Chicago
Han, B., Zhou, X., Zhang, R., Zang, W., Chen, Z., Song, H., Wan, H., Zheng, C."Mutations of the epidermal growth factor receptor gene in NSCLC patients". Oncology Letters 2, no. 6 (2011): 1233-1237. https://doi.org/10.3892/ol.2011.366
Copy and paste a formatted citation
x
Spandidos Publications style
Han B, Zhou X, Zhang R, Zang W, Chen Z, Song H, Wan H and Zheng C: Mutations of the epidermal growth factor receptor gene in NSCLC patients. Oncol Lett 2: 1233-1237, 2011.
APA
Han, B., Zhou, X., Zhang, R., Zang, W., Chen, Z., Song, H. ... Zheng, C. (2011). Mutations of the epidermal growth factor receptor gene in NSCLC patients. Oncology Letters, 2, 1233-1237. https://doi.org/10.3892/ol.2011.366
MLA
Han, B., Zhou, X., Zhang, R., Zang, W., Chen, Z., Song, H., Wan, H., Zheng, C."Mutations of the epidermal growth factor receptor gene in NSCLC patients". Oncology Letters 2.6 (2011): 1233-1237.
Chicago
Han, B., Zhou, X., Zhang, R., Zang, W., Chen, Z., Song, H., Wan, H., Zheng, C."Mutations of the epidermal growth factor receptor gene in NSCLC patients". Oncology Letters 2, no. 6 (2011): 1233-1237. https://doi.org/10.3892/ol.2011.366
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