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Article

hTERT methylation is necessary but not sufficient for telomerase activity in colorectal cells

  • Authors:
    • Cristina Valls-Bautista
    • Stéphanie Bougel
    • Carme  Piñol‑Felis
    • Joan Viñas-Salas
    • Jean Benhattar
  • View Affiliations / Copyright

    Affiliations: Department of Medicine, Medical School, Lleida University, Lleida, Spain, Institute of Pathology, CHUV and University of Lausanne, 1011 Lausanne, Switzerland
  • Pages: 1257-1260
    |
    Published online on: August 18, 2011
       https://doi.org/10.3892/ol.2011.386
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Abstract

Colorectal cancers exhibit a high telomerase activity, usually correlated with the hypermethylation of the promoter of its hTERT catalytic subunit. Although telome-rase is not expressed in normal tissue, certain proliferative somatic cells such as intestinal crypt cells have demonstrated telomerase activity. The aim of this study was to determine whether a correlation exists between telomerase activity, levels of hTERT methylation and telomere length in tumoral and normal colorectal tissues. Tumor, transitional and normal tissues were obtained from 11 patients with a colorectal cancer. After bisulfite modification of genomic DNA, hTERT promoter methylation was analyzed by methylation‑sensitive single‑strand conformation analysis (MS-SSCA). Telomerase activity and telomere length were measured by a fluorescent‑telomeric repeat amplification protocol assay and by Southern blotting, respectively. A significant increase of hTERT methy-lation and telomerase activity, and a reduction of the mean telomere length were observed in the tumor tissues compared to the transitional and normal mucosa. In the transitional and normal mucosa, telomerase activity was significantly lower than that in tumor tissues, even with high levels of hTERT methylation. Nevertheless, hTERT promoter methylation was not linearly correlated to telomerase activity. These data indicate that hTERT promoter methylation is a necessary event for hTERT expression, as is telomerase activity. However, methy-lation is not sufficient for hTERT activation, particularly in normal colorectal cells.
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Copy and paste a formatted citation
Spandidos Publications style
Valls-Bautista C, Bougel S, Piñol‑Felis C, Viñas-Salas J and Benhattar J: hTERT methylation is necessary but not sufficient for telomerase activity in colorectal cells. Oncol Lett 2: 1257-1260, 2011.
APA
Valls-Bautista, C., Bougel, S., Piñol‑Felis, C., Viñas-Salas, J., & Benhattar, J. (2011). hTERT methylation is necessary but not sufficient for telomerase activity in colorectal cells. Oncology Letters, 2, 1257-1260. https://doi.org/10.3892/ol.2011.386
MLA
Valls-Bautista, C., Bougel, S., Piñol‑Felis, C., Viñas-Salas, J., Benhattar, J."hTERT methylation is necessary but not sufficient for telomerase activity in colorectal cells". Oncology Letters 2.6 (2011): 1257-1260.
Chicago
Valls-Bautista, C., Bougel, S., Piñol‑Felis, C., Viñas-Salas, J., Benhattar, J."hTERT methylation is necessary but not sufficient for telomerase activity in colorectal cells". Oncology Letters 2, no. 6 (2011): 1257-1260. https://doi.org/10.3892/ol.2011.386
Copy and paste a formatted citation
x
Spandidos Publications style
Valls-Bautista C, Bougel S, Piñol‑Felis C, Viñas-Salas J and Benhattar J: hTERT methylation is necessary but not sufficient for telomerase activity in colorectal cells. Oncol Lett 2: 1257-1260, 2011.
APA
Valls-Bautista, C., Bougel, S., Piñol‑Felis, C., Viñas-Salas, J., & Benhattar, J. (2011). hTERT methylation is necessary but not sufficient for telomerase activity in colorectal cells. Oncology Letters, 2, 1257-1260. https://doi.org/10.3892/ol.2011.386
MLA
Valls-Bautista, C., Bougel, S., Piñol‑Felis, C., Viñas-Salas, J., Benhattar, J."hTERT methylation is necessary but not sufficient for telomerase activity in colorectal cells". Oncology Letters 2.6 (2011): 1257-1260.
Chicago
Valls-Bautista, C., Bougel, S., Piñol‑Felis, C., Viñas-Salas, J., Benhattar, J."hTERT methylation is necessary but not sufficient for telomerase activity in colorectal cells". Oncology Letters 2, no. 6 (2011): 1257-1260. https://doi.org/10.3892/ol.2011.386
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