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Article

Factors affecting the sensitivity of human-derived esophageal carcinoma cell lines to 5-fluorouracil and cisplatin

  • Authors:
    • Tetsuya Minegaki
    • Kohji Takara
    • Ryohei Hamaguchi
    • Masayuki Tsujimoto
    • Kohshi Nishiguchi
  • View Affiliations / Copyright

    Affiliations: Department of Clinical Pharmacy, Faculty of Pharmaceutical Sciences, Kyoto Pharmaceutical University, Kyoto 607-8414, Japan, Department of Clinical Pharmaceutics, Faculty of Pharmaceutical Sciences, Himeji Dokkyo University, Himeji 670-8524, Japan
  • Pages: 427-434
    |
    Published online on: November 5, 2012
       https://doi.org/10.3892/ol.2012.1014
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Abstract

Effective chemotherapy against esophageal carcinoma is considered achievable with a combination of 5-fluorouracil (5-FU) and cisplatin (CDDP). However, chemotherapy remains ineffective in certain patients. The aim of this study was to clarify the factors which affect sensitivity to 5-FU and CDDP. The effects of factors known to influence sensitivity to 5-FU and CDDP, namely transporters, DNA repair enzymes and metabolic enzymes, were examined. mRNA levels of four transporters, SLC22A2, SLC23A2, ABCB1 and ABCC2, two DNA repair-related enzymes, Rad51 and MSH2, and one metabolic enzyme, dihydropyrimidine dehydrogenase (DPYD), showed a strong correlation (|r|>0.7) with IC50 values for 5-FU. In addition, the mRNA levels of ABCC2, MSH2 and DPYD showed a strong correlation (|r|>0.7) with the IC50 values for CDDP. Gimeracil, a DPYD inhibitor, enhanced the sensitivity of some cells to 5-FU but decreased the sensitivity of all the cells to CDDP. The inhibitory effects of ABCC2 with MK571 did not correspond to those observed in the correlation analysis. In conclusion, mRNA levels of SLC22A2, SLC23A2, ABCB1, ABCC2, Rad51, MSH2 and DPYD were confirmed to be strongly correlated with IC50 values for 5-FU, and mRNA levels of ABCC2, MSH2 and DPYD were confirmed to be strongly correlated with IC50 values for CDDP. In addition, the inhibition of DPYD appeared to affect the cytotoxicity of CDDP.
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View References

1. 

National Cancer Center: Cancer statistics in Japan: 2011, http://ganjoho.jp/public/statistics/backnumber/2011_jp.html. Accessed June 29, 2012.

2. 

Tahara M, Ohtsu A, Hironaka S, Boku N, Ishikura S, Miyata Y, Ogino T and Yoshida S: Clinical impact of criteria for complete response (CR) of primary site to treatment of esophageal cancer. Jpn J Clin Oncol. 35:316–323. 2005. View Article : Google Scholar : PubMed/NCBI

3. 

Pratt S, Shepard RL, Kandasamy RA, Johnston PA, Perry W III and Dantzig AH: The multidrug resistance protein 5 (ABCC5) confers resistance to 5-fluorouracil and transports its monophosphorylated metabolites. Mol Cancer Ther. 4:855–863. 2005. View Article : Google Scholar : PubMed/NCBI

4. 

Kornmann M, Schwabe W, Sander S, Kron M, Sträter J, Polat S, Kettner E, Weiser HF, Baumann W, Schramm H, Häusler P, Ott K, Behnke D, Staib L, Beger HG and Link KH: Thymidylate synthase and dihydropyrimidine dehydrogenase mRNA expression levels: predictors for survival in colorectal cancer patients receiving adjuvant 5-fluorouracil. Clin Cancer Res. 9:4116–4124. 2003.

5. 

Ciaparrone M, Quirino M, Schinzari G, Zannoni G, Corsi DC, Vecchio FM, Cassano A, La Torre G and Barone C: Predictive role of thymidylate synthase, dihydropyrimidine dehydrogenase and thymidine phosphorylase expression in colorectal cancer patients receiving adjuvant 5-fluorouracil. Oncology. 70:366–377. 2006. View Article : Google Scholar

6. 

Larminat F and Bohr VA: Role of the human ERCC-1 gene in gene-specific repair of cisplatin-induced DNA damage. Nucleic Acids Res. 22:3005–3010. 1994. View Article : Google Scholar : PubMed/NCBI

7. 

Andersson BS, Sadeghi T, Siciliano MJ, Legerski R and Murray D: Nucleotide excision repair genes as determinants of cellular sensitivity to cyclophosphamide analogs. Cancer Chemother Pharmacol. 38:406–416. 1996. View Article : Google Scholar : PubMed/NCBI

8. 

Hsu DS, Lan HY, Huang CH, Tai SK, Chang SY, Tsai TL, Chang CC, Tzeng CH, Wu KJ, Kao JY and Yang MH: Regulation of excision repair cross-complementation group 1 by Snail contributes to cisplatin resistance in head and neck cancer. Clin Cancer Res. 16:4561–4571. 2010. View Article : Google Scholar : PubMed/NCBI

9. 

Shimada Y, Imamura M, Wagata T, Yamaguchi N and Tobe T: Characterization of 21 newly established esophageal cancer cell lines. Cancer. 69:277–284. 1992. View Article : Google Scholar : PubMed/NCBI

10. 

Kitada N, Takara K, Minegaki T, Itoh C, Tsujimoto M, Sakaeda T and Yokoyama T: Factors affecting sensitivity to antitumor platinum derivatives of human colorectal tumor cell lines. Cancer Chemother Pharmacol. 62:577–584. 2008. View Article : Google Scholar : PubMed/NCBI

11. 

Kimura N, Masuda S, Tanihara Y, Ueo H, Okuda M, Katsura T and Inui K: Metformin is a superior substrate for renal organic cation transporter OCT2 rather than hepatic OCT1. Drug Metab Pharmacokinet. 20:379–386. 2005. View Article : Google Scholar : PubMed/NCBI

12. 

Filipski KK, Loos WJ, Verweij J and Sparreboom A: Interaction of Cisplatin with the human organic cation transporter 2. Clin Cancer Res. 14:3875–3880. 2008. View Article : Google Scholar : PubMed/NCBI

13. 

Karasawa H, Miura K, Fujibuchi W, Ishida K, Kaneko N, Kinouchi M, Okabe M, Ando T, Murata Y, Sasaki H, Takami K, Yamamura A, Shibata C and Sasaki I: Down-regulation of cIAP2 enhances 5-FU sensitivity through the apoptotic pathway in human colon cancer cells. Cancer Sci. 100:903–913. 2009. View Article : Google Scholar : PubMed/NCBI

14. 

Takara K, Obata Y, Yoshikawa E, Kitada N, Sakaeda T, Ohnishi N and Yokoyama T: Molecular changes to HeLa cells on continuous exposure to cisplatin or paclitaxel. Cancer Chemother Pharmacol. 58:785–793. 2006. View Article : Google Scholar : PubMed/NCBI

15. 

Takenaka T, Yoshino I, Kouso H, Ohba T, Yohena T, Osoegawa A, Shoji F and Maehara Y: Combined evaluation of Rad51 and ERCC1 expressions for sensitivity to platinum agents in non-small cell lung cancer. Int J Cancer. 121:895–900. 2007. View Article : Google Scholar : PubMed/NCBI

16. 

Noma B, Sasaki T, Fujimoto Y, Serikawa M, Kobayashi K, Inoue M, Itsuki H, Kamigaki M, Minami T and Chayama K: Expression of multidrug resistance-associated protein 2 is involved in chemotherapy resistance in human pancreatic cancer. Int J Oncol. 33:1187–1194. 2008.PubMed/NCBI

17. 

Lan L, Hayashi T, Rabeya RM, Nakajima S, Kanno S, Takao M, Matsunaga T, Yoshino M, Ichikawa M, Riele H, Tsuchiya S, Tanaka K and Yasui A: Functional and physical interactions between ERCC1 and MSH2 complexes for resistance to cisdiamminedichloroplatinum(II) in mammalian cells. DNA Repair (Amst). 3:135–143. 2004. View Article : Google Scholar : PubMed/NCBI

18. 

Takizawa M, Kawakami K, Obata T, Matsumoto I, Ohta Y, Oda M, Sasaki T and Watanabe G: In vitro sensitivity to platinum-derived drugs is associated with expression of thymidylate synthase and dihydropyrimidine dehydrogenase in human lung cancer. Oncol Rep. 15:1533–1539. 2006.

19. 

Pettersen HS, Visnes T, Vågbø CB, Svaasand EK, Doseth B, Slupphaug G, Kavli B and Krokan HE: UNG-initiated base excision repair is the major repair route for 5-fluorouracil in DNA, but 5-fluorouracil cytotoxicity depends mainly on RNA incorporation. Nucleic Acids Res. 39:8430–8444. 2011. View Article : Google Scholar : PubMed/NCBI

20. 

Li Y, Mizutani Y, Shiraishi T, Nakamura T, Mikami K, Takaha N, Okihara K, Kawauchi A, Sakai T and Miki T: The significance of the expression of dihydropyrimidine dehydrogenase in prostate cancer. BJU Int. 99:663–668. 2007. View Article : Google Scholar : PubMed/NCBI

21. 

Ando T, Ishiguro H, Kuwabara Y, Kimura M, Mitsui A, Sugito N, Mori R, Ogawa R, Katada T and Fujii Y: Relationship between expression of 5-fluorouracil metabolic enzymes and 5-fluorouracil sensitivity in esophageal carcinoma cell lines. Dis Esophagus. 21:15–20. 2008.PubMed/NCBI

22. 

Pedersen JM, Matsson P, Bergström CA, Norinder U, Hoogstraate J and Artursson P: Prediction and identification of drug interactions with the human ATP-binding cassette transporter multidrug-resistance associated protein 2 (MRP2; ABCC2). J Med Chem. 51:3275–3287. 2008. View Article : Google Scholar : PubMed/NCBI

23. 

Reidling JC, Subramanian VS, Dahhan T, Sadat M and Said HM: Mechanisms and regulation of vitamin C uptake: studies of the hSVCT systems in human liver epithelial cells. Am J Physiol Gastrointest Liver Physiol. 295:1217–1227. 2008. View Article : Google Scholar : PubMed/NCBI

24. 

Nishimura M, Koeda A, Morikawa H, Satoh T, Narimatsu S and Naito S: Comparison of inducibility of multidrug resistance (MDR)1, multidrug resistance-associated protein (MRP)1, and MRP2 mRNAs by prototypical microsomal enzyme inducers in primary cultures of human and cynomolgus monkey hepatocytes. Biol Pharm Bull. 31:2068–2072. 2008. View Article : Google Scholar

25. 

Maubon N, Le Vee M, Fossati L, Audry M, Le Ferrec E, Bolze S and Fardel O: Analysis of drug transporter expression in human intestinal Caco-2 cells by real-time PCR. Fundam Clin Pharmacol. 21:659–663. 2007. View Article : Google Scholar : PubMed/NCBI

26. 

Dauchy S, Miller F, Couraud PO, Weaver RJ, Weksler B, Romero IA, Scherrmann JM, De Waziers I and Declèves X: Expression and transcriptional regulation of ABC transporters and cytochromes P450 in hCMEC/D3 human cerebral micro-vascular endothelial cells. Biochem Pharmacol. 77:897–909. 2009. View Article : Google Scholar

27. 

Shimizu J, Horio Y, Osada H, Hida T, Hasegawa Y, Shimokata K, Takahashi T, Sekido Y and Yatabe Y: mRNA expression of RRM1, ERCC1 and ERCC2 is not associated with chemosensitivity to cisplatin, carboplatin and gemcitabine in human lung cancer cell lines. Respirology. 13:510–517. 2008. View Article : Google Scholar : PubMed/NCBI

28. 

Sliwinski T, Krupa R, Majsterek I, Rykala J, Kolacinska A, Morawiec Z, Drzewoski J, Zadrozny M and Blasiak J: Polymorphisms of the BRCA2 and RAD51 genes in breast cancer. Breast Cancer Res Treat. 94:105–109. 2005. View Article : Google Scholar : PubMed/NCBI

29. 

Amirrad M, Al-Mulla F, Varadharaj G, John B, Saji T and Anim JT: BRCA1 gene expression in breast cancer in Kuwait: correlation with prognostic parameters. Med Princ Pract. 14:67–72. 2005. View Article : Google Scholar : PubMed/NCBI

30. 

Müller A, Zielinski D, Friedrichs N, Oberschmid B, Merkelbach-Bruse S, Schackert HK, Linnebacher M, von Knebel Doeberitz M, Büttner R and Rüschoff J: Reduced mRNA expression in paraffin-embedded tissue identifies MLH1-and MSH2-deficient colorectal tumours and potential mutation carriers. Virchows Arch. 453:9–16. 2008.PubMed/NCBI

31. 

Veeriah S, Kautenburger T, Habermann N, Sauer J, Dietrich H, Will F and Pool-Zobel BL: Apple flavonoids inhibit growth of HT29 human colon cancer cells and modulate expression of genes involved in the biotransformation of xenobiotics. Mol Carcinog. 45:164–174. 2006. View Article : Google Scholar : PubMed/NCBI

32. 

Uthus EO, Reeves PG and Saari JT: Copper deficiency decreases plasma homocysteine in rats. J Nutr. 137:1370–1374. 2007.PubMed/NCBI

33. 

Hoang YD, Avakian AP and Luderer U: Minimal ovarian upregulation of glutamate cysteine ligase expression in response to suppression of glutathione by buthionine sulfoximine. Reprod Toxicol. 21:186–196. 2006. View Article : Google Scholar : PubMed/NCBI

34. 

Gustavsson B, Kaiser C, Carlsson G, Wettergren Y, Odin E, Lindskog EB, Niyikiza C and Ma D: Molecular determinants of efficacy for 5-FU-based treatments in advanced colorectal cancer: mRNA expression for 18 chemotherapy-related genes. Int J Cancer. 124:1220–1226. 2009. View Article : Google Scholar : PubMed/NCBI

35. 

Yoshinare K, Kubota T, Watanabe M, Wada N, Nishibori H, Hasegawa H, Kitajima M, Takechi T and Fukushima M: Gene expression in colorectal cancer and in vitro chemosensitivity to 5-fluorouracil: a study of 88 surgical specimens. Cancer Sci. 94:633–638. 2003. View Article : Google Scholar : PubMed/NCBI

36. 

Matsubara J, Nishina T, Yamada Y, Moriwaki T, Shimoda T, Kajiwara T, Nakajima TE, Kato K, Hamaguchi T, Shimada Y, Okayama Y, Oka T and Shirao K: Impacts of excision repair cross-complementing gene 1 (ERCC1), dihydropyrimidine dehydrogenase, and epidermal growth factor receptor on the outcomes of patients with advanced gastric cancer. Br J Cancer. 98:832–839. 2008. View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Minegaki T, Takara K, Hamaguchi R, Tsujimoto M and Nishiguchi K: Factors affecting the sensitivity of human-derived esophageal carcinoma cell lines to 5-fluorouracil and cisplatin. Oncol Lett 5: 427-434, 2013.
APA
Minegaki, T., Takara, K., Hamaguchi, R., Tsujimoto, M., & Nishiguchi, K. (2013). Factors affecting the sensitivity of human-derived esophageal carcinoma cell lines to 5-fluorouracil and cisplatin. Oncology Letters, 5, 427-434. https://doi.org/10.3892/ol.2012.1014
MLA
Minegaki, T., Takara, K., Hamaguchi, R., Tsujimoto, M., Nishiguchi, K."Factors affecting the sensitivity of human-derived esophageal carcinoma cell lines to 5-fluorouracil and cisplatin". Oncology Letters 5.2 (2013): 427-434.
Chicago
Minegaki, T., Takara, K., Hamaguchi, R., Tsujimoto, M., Nishiguchi, K."Factors affecting the sensitivity of human-derived esophageal carcinoma cell lines to 5-fluorouracil and cisplatin". Oncology Letters 5, no. 2 (2013): 427-434. https://doi.org/10.3892/ol.2012.1014
Copy and paste a formatted citation
x
Spandidos Publications style
Minegaki T, Takara K, Hamaguchi R, Tsujimoto M and Nishiguchi K: Factors affecting the sensitivity of human-derived esophageal carcinoma cell lines to 5-fluorouracil and cisplatin. Oncol Lett 5: 427-434, 2013.
APA
Minegaki, T., Takara, K., Hamaguchi, R., Tsujimoto, M., & Nishiguchi, K. (2013). Factors affecting the sensitivity of human-derived esophageal carcinoma cell lines to 5-fluorouracil and cisplatin. Oncology Letters, 5, 427-434. https://doi.org/10.3892/ol.2012.1014
MLA
Minegaki, T., Takara, K., Hamaguchi, R., Tsujimoto, M., Nishiguchi, K."Factors affecting the sensitivity of human-derived esophageal carcinoma cell lines to 5-fluorouracil and cisplatin". Oncology Letters 5.2 (2013): 427-434.
Chicago
Minegaki, T., Takara, K., Hamaguchi, R., Tsujimoto, M., Nishiguchi, K."Factors affecting the sensitivity of human-derived esophageal carcinoma cell lines to 5-fluorouracil and cisplatin". Oncology Letters 5, no. 2 (2013): 427-434. https://doi.org/10.3892/ol.2012.1014
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