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Article

Expression of stem cell factor in gastrointestinal stromal tumors: Implications for proliferation and imatinib resistance

  • Authors:
    • Xiao-Wei Hou
    • Chen-Guang Bai
    • Xiao-Hong Liu
    • Cen Qiu
    • Ling Huang
    • Jing-Jing Xu
    • Da-Lie Ma
  • View Affiliations / Copyright

    Affiliations: Department of Pathology, Changhai Hospital, Second Military Medical University, Shanghai 200433, P.R. China, Institute of Cardiothoracic Surgery, Changhai Hospital, Second Military Medical University, Shanghai 200433, P.R. China
  • Pages: 552-558
    |
    Published online on: November 9, 2012
       https://doi.org/10.3892/ol.2012.1019
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Abstract

KIT autophosphorylation caused by mutation of KIT is considered to be a critical mechanism for the oncogenesis of gastrointestinal stromal tumors (GISTs). However, little is known regarding whether stem cell factor (SCF), the KIT ligand, is able to induce the proliferation of GIST cells by activating the wild-type KIT receptor in GISTs. Imatinib, a tyrosine kinase inhibitor, has been demonstrated to be effective as treatment for the majority of GISTs. However, primary resistance to imatinib in GISTs with wild-type KIT and acquired resistance in GISTs with mutant KIT are becoming increasingly significant problems. The aims of this study were to detect the expression and function of SCF in 68 GIST samples, and to explore the relationship between SCF activity and imatinib resistance using immunohistochemical staining and western blot analysis. Results showed abundant expression of SCF in GISTs and demonstrated that SCF is capable of enhancing GIST cell proliferation. Similar to its ineffectiveness in wild‑type GISTs, imatinib also failed to inhibit SCF‑induced KIT activation in GISTs with mutant KIT. We also found increased SCF expression in GIST cells treated with imatinib. Overall, our results indicated that SCF‑induced KIT activation is a novel essential pathway for the proliferation of GISTs. Imatinib was not able to inhibit the activity of SCF, while it promoted the expression of SCF, which may have contributed to acquired imatinib resistance.
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Copy and paste a formatted citation
Spandidos Publications style
Hou X, Bai C, Liu X, Qiu C, Huang L, Xu J and Ma D: Expression of stem cell factor in gastrointestinal stromal tumors: Implications for proliferation and imatinib resistance. Oncol Lett 5: 552-558, 2013.
APA
Hou, X., Bai, C., Liu, X., Qiu, C., Huang, L., Xu, J., & Ma, D. (2013). Expression of stem cell factor in gastrointestinal stromal tumors: Implications for proliferation and imatinib resistance. Oncology Letters, 5, 552-558. https://doi.org/10.3892/ol.2012.1019
MLA
Hou, X., Bai, C., Liu, X., Qiu, C., Huang, L., Xu, J., Ma, D."Expression of stem cell factor in gastrointestinal stromal tumors: Implications for proliferation and imatinib resistance". Oncology Letters 5.2 (2013): 552-558.
Chicago
Hou, X., Bai, C., Liu, X., Qiu, C., Huang, L., Xu, J., Ma, D."Expression of stem cell factor in gastrointestinal stromal tumors: Implications for proliferation and imatinib resistance". Oncology Letters 5, no. 2 (2013): 552-558. https://doi.org/10.3892/ol.2012.1019
Copy and paste a formatted citation
x
Spandidos Publications style
Hou X, Bai C, Liu X, Qiu C, Huang L, Xu J and Ma D: Expression of stem cell factor in gastrointestinal stromal tumors: Implications for proliferation and imatinib resistance. Oncol Lett 5: 552-558, 2013.
APA
Hou, X., Bai, C., Liu, X., Qiu, C., Huang, L., Xu, J., & Ma, D. (2013). Expression of stem cell factor in gastrointestinal stromal tumors: Implications for proliferation and imatinib resistance. Oncology Letters, 5, 552-558. https://doi.org/10.3892/ol.2012.1019
MLA
Hou, X., Bai, C., Liu, X., Qiu, C., Huang, L., Xu, J., Ma, D."Expression of stem cell factor in gastrointestinal stromal tumors: Implications for proliferation and imatinib resistance". Oncology Letters 5.2 (2013): 552-558.
Chicago
Hou, X., Bai, C., Liu, X., Qiu, C., Huang, L., Xu, J., Ma, D."Expression of stem cell factor in gastrointestinal stromal tumors: Implications for proliferation and imatinib resistance". Oncology Letters 5, no. 2 (2013): 552-558. https://doi.org/10.3892/ol.2012.1019
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