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Article

Analgesic-antitumor peptide induces apoptosis and inhibits the proliferation of SW480 human colon cancer cells

  • Authors:
    • Yu Gu
    • Shen-Lin Liu
    • Wen-Zheng Ju
    • Chang-Yin Li
    • Peng Cao
  • View Affiliations / Copyright

    Affiliations: The First Clinical Medical College, Nanjing University of Traditional Medicine, Nanjing, Jiangsu, P.R. China, Laboratory of Clinical Pharmacokinetics of Traditional Chinese Medicine, Jiangsu Province Hospital of Traditional Chinese Medicine, Nanjing, Jiangsu, P.R. China, Laboratory of Cellular and Molecular Biology, Jiangsu Province Institute of Traditional Chinese Medicine, Nanjing, Jiangsu, P.R. China
  • Pages: 483-488
    |
    Published online on: November 27, 2012
       https://doi.org/10.3892/ol.2012.1049
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Abstract

Colorectal cancer is one of the most common malignant tumors, and is associated with significant morbidity and mortality. In this study, recombinant analgesic-antitumor peptide (rAGAP), a protein consisting of small ubiquitin-related modifier (SUMO) linked with a hexa-histidine tag, was used as an antitumor analgesic peptide. The purpose of the present study was to investigate the antitumor activity of rAGAP in human colon adenocarcinoma SW480 cells and its potential molecular mechanisms of action. In this study, cell viability and apoptosis of rAGAP-treated SW480 cells was evaluated by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, flow cytometry and 4',6-diamidino-2-phenylindole (DAPI) staining. Western blotting was used to investigate the effects of rAGAP on p27, Bcl-2/Bax and PTEN/PI3K/Akt cellular signal transduction. Our results showed that rAGAP not only enhanced apoptosis, but also inhibited the proliferation of SW480 cells. rAGAP upregulates the expression of p27 in SW480 cells and leads to cell cycle arrest in the G1 phase. Furthermore, rAGAP significantly increases the production of Bax and PTEN and suppresses the activation of Bcl-2, phosphatidylinositol 3-kinase (PI3K) and phospho-Akt (p-Akt) in SW480 cells. These results suggest that rAGAP may be a potential new anti-colorectal cancer drug.
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Copy and paste a formatted citation
Spandidos Publications style
Gu Y, Liu S, Ju W, Li C and Cao P: Analgesic-antitumor peptide induces apoptosis and inhibits the proliferation of SW480 human colon cancer cells. Oncol Lett 5: 483-488, 2013.
APA
Gu, Y., Liu, S., Ju, W., Li, C., & Cao, P. (2013). Analgesic-antitumor peptide induces apoptosis and inhibits the proliferation of SW480 human colon cancer cells. Oncology Letters, 5, 483-488. https://doi.org/10.3892/ol.2012.1049
MLA
Gu, Y., Liu, S., Ju, W., Li, C., Cao, P."Analgesic-antitumor peptide induces apoptosis and inhibits the proliferation of SW480 human colon cancer cells". Oncology Letters 5.2 (2013): 483-488.
Chicago
Gu, Y., Liu, S., Ju, W., Li, C., Cao, P."Analgesic-antitumor peptide induces apoptosis and inhibits the proliferation of SW480 human colon cancer cells". Oncology Letters 5, no. 2 (2013): 483-488. https://doi.org/10.3892/ol.2012.1049
Copy and paste a formatted citation
x
Spandidos Publications style
Gu Y, Liu S, Ju W, Li C and Cao P: Analgesic-antitumor peptide induces apoptosis and inhibits the proliferation of SW480 human colon cancer cells. Oncol Lett 5: 483-488, 2013.
APA
Gu, Y., Liu, S., Ju, W., Li, C., & Cao, P. (2013). Analgesic-antitumor peptide induces apoptosis and inhibits the proliferation of SW480 human colon cancer cells. Oncology Letters, 5, 483-488. https://doi.org/10.3892/ol.2012.1049
MLA
Gu, Y., Liu, S., Ju, W., Li, C., Cao, P."Analgesic-antitumor peptide induces apoptosis and inhibits the proliferation of SW480 human colon cancer cells". Oncology Letters 5.2 (2013): 483-488.
Chicago
Gu, Y., Liu, S., Ju, W., Li, C., Cao, P."Analgesic-antitumor peptide induces apoptosis and inhibits the proliferation of SW480 human colon cancer cells". Oncology Letters 5, no. 2 (2013): 483-488. https://doi.org/10.3892/ol.2012.1049
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