In vivo evaluation of the synthesized novel 2-benzyloxybenzaldehyde analog CCY-1a-E2 for the treatment of leukemia in the BALB/c mouse WEHI-3 allograft model

Lin,Chingju; Yang,Jai-Sing; Tsai,Shih-Chang; Lin,Chin-Fen; Lee,Miau-Rong

doi:10.3892/ol.2012.1079

In vivo evaluation of the synthesized novel 2-benzyloxybenzaldehyde analog CCY-1a-E2 for the treatment of leukemia in the BALB/c mouse WEHI-3 allograft model

Authors:
- Chingju Lin
- Jai-Sing Yang
- Shih-Chang Tsai
- Chin-Fen Lin
- Miau-Rong Lee
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Affiliations: Department of Physiology, China Medical University, Taichung 40402, Taiwan, R.O.C., Department of Pharmacology, China Medical University, Taichung 40402, Taiwan, R.O.C., Department of Biological Science and Technology, China Medical University, Taichung 40402, Taiwan, R.O.C., Department of Biochemistry, China Medical University, Taichung 40402, Taiwan, R.O.C.
Published online on: December 17, 2012 https://doi.org/10.3892/ol.2012.1079
Pages: 777-782

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Abstract

Our previous study demonstrated that the 2-benzyloxybenzaldehyde analog CCY-1a-E2 is a potent compound against HL-60 human leukemia cell lines. To investigate the potential therapeutic application of CCY-1a-E2 for leukemia, we analyzed the antileukemic effects and safety of CCY-1a-E2 in the BALB/c mouse WEHI-3 allograft model. Our results showed that CCY-1a-E2 decreased the percentage of viable cells in a concentration-dependent manner. The IC50 of CCY-1a-E2 was 5 µM for the 24-h treatment of WEHI-3 cells. We examined the antileukemic activity of CCY-1a-E2 in the BALB/c mouse WEHI-3 allograft model. The CCY-1a-E2 (100 mg/kg) group was not found to have significantly decreased body weight compared with the control group, while the leukemia group was found to have significantly decreased body weight compared with the control mice. The CCY-1a-E2 (100 mg/kg) group showed no difference in spleen and liver weight, but significantly decreased levels of CD11b and CD45 compared with the leukemia group. In safety evaluation analysis, CCY-1a-E2 had no adverse effects on renal, hepatic and hematological parameters. Based on these observations, CCY-1a-E2 has efficacious antileukemic activity in the BALB/c mouse WEHI-3 allograft model.

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