Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Letters
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-1074 Online ISSN: 1792-1082
Journal Cover
March 2013 Volume 5 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
March 2013 Volume 5 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Isolation and enrichment of PC-3 prostate cancer stem-like cells using MACS and serum-free medium

  • Authors:
    • Xia Sheng
    • Zeng Li
    • De-Lin Wang
    • Wen-Bin Li
    • Zhao Luo
    • Ke-Hong Chen
    • Jian-Jia Cao
    • Chao Yu
    • Wu-Jiang Liu
  • View Affiliations / Copyright

    Affiliations: Department of Urology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, P.R. China, Life Science Research Institute of Chongqing Medical University, Chongqing 400016, P.R. China, Institute of Urology, Peking University First Hospital Street 8 of Xishiku, Xicheng, Beijing 100034, P.R. China
  • Pages: 787-792
    |
    Published online on: December 21, 2012
       https://doi.org/10.3892/ol.2012.1090
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Prostate cancer stem-like cells (PCSLCs) are considered to be the ‘seed’ of prostate cancer. The aim of this study was to confirm that the PC‑3 cells, which we isolated and enriched from PC-3 cells through magnetic bead cell sorting (MACS) and serum-free medium (SFM) culture, were PCSLCs. Combinations of MACS, flow cytometry (FCM), SFM and immunocytochemistry (ICC) were used to ensure the positive expression of CD133 and CD44 on PC‑3 and sphere‑forming cell membranes. Self-renewal, multi-potential differentiation, unlimited proliferation and permanency assays were also applied to indentify whether the PC‑3 cells exhibited the characteristics of cancer stem cells (CSCs). As a result, there was a low proportion of PCSLCs in the PC-3 cells. In the FCM assay, the proportion of cells expressing CD133 or CD44 in the PC-3 cells was 0.51 and 0.31%, respectively. In addition, we found that the proportion of PC‑3 cells sorted by MACS that expressed CD133 was significantly increased compared with that of the sphere‑forming cells cultured in SFM (99.09 vs. 84.80%, P<0.05), while no difference was observed in the proportion of cells expressing CD44 between them (99.88 vs. 99.82%, P>0.05). The expression of PAP and AR as detected by western blot analysis of induced PCSLCs was significantly increased compared with that of uninduced PCSLCs (P<0.05); the proliferation capacity of PCSLCs was significantly higher than that of both the PC-3 cells (P<0.05) and induced PCSLCs (P<0.05). Furthermore, the PCSLCs that were isolated from SFM and MACS both demonstrated certain characteristics of stem cells and should be considered as stem cell‑like. These data may hold potential for further exploring the role of PCSLCs.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

View References

1 

Dean M, Fojo T and Bates S: Tumor stem cells and drug resistance. Nat Rev Cancer. 5:275–284. 2005. View Article : Google Scholar

2 

Hadnagy A, Gaboury L, Beaulieu R and Balicki D: SP analysis may be used to identify cancer stem cell populations. Exp Cell Res. 312:701–710. 2006. View Article : Google Scholar

3 

Piccirillo SG and Vescovi AL: Brain tumor stem cells: possibilities of new therapeutic strategies. Expert Opin Biol Ther. 7:1129–1135. 2007. View Article : Google Scholar : PubMed/NCBI

4 

Collins AT, Berry PA, Hyde C, Stower MJ and Maitland NJ: Prospective identification of tumorigenic prostate cancer stem cells. Cancer Res. 65:10946–10951. 2005. View Article : Google Scholar : PubMed/NCBI

5 

Bonnet D and Dick JE: Human acute myeloid leukemia is organized as a hierarchy that originates from a primitive hematopoietic cell. Nat Med. 3:730–737. 1997. View Article : Google Scholar : PubMed/NCBI

6 

Clarke MF, Morrison SJ, Wicha MS, et al: Isolation and use of solid tumor stem cells. US Patent Application 119565. Filed December 28, 2002; issued April 21, 2011.

7 

Suetsugu A, Nagaki M, Aoki H, et al: Characterization of CD133+ hepatocellular carcinoma cells as cancer stem/progenitor cells. Biochem Biophys Res Commun. 351:820–824. 2006.

8 

O’Brien CA, Pollett A, Gallinger S and Dick JE: A human colon cancer cell capable of initiating tumour growth in immunodeficient mice. Nature. 445:106–110. 2007.PubMed/NCBI

9 

Gong C, Liao H, Guo F, et al: Implication of expression of Nanog in prostate cancer cells and their stem cell. J Huazhong Univ Sci Technolog Med Sci. 32:242–246. 2012. View Article : Google Scholar : PubMed/NCBI

10 

Dubrovska A, Elliott J, Salamone RJ, et al: CXCR4 expression in prostate cancer progenitor cells. PLoS One. 7:e312262012. View Article : Google Scholar : PubMed/NCBI

11 

Fan X, Liu S, Su F, et al: Effective enrichment of prostate cancer stem cells from spheres in a suspension culture system. Urol Oncol. 30:314–318. 2012. View Article : Google Scholar : PubMed/NCBI

12 

Lu RQ, Wu JG, Zhou GC, et al: Sorting of CD133(+) subset cells in human gastric cancer and the identification of their tumor initiating cell-like properties. Zhonghua Wei Chang Wai Ke Za Zhi. 15:174–179. 2012.(In Chinese).

13 

He JQ, Vu DM, Hunt G, et al: Human cardiac stem cells isolated from atrial appendages stably express c-kit. PLoS One. 6:e277192011. View Article : Google Scholar : PubMed/NCBI

14 

Tang B, Yoo N, Vu M, et al: Transforming growth factor-beta can suppress tumorigenesis through effects on the putative cancer stem or early progenitor cell and committed progeny in a breast cancer xenograft model. Cancer Res. 67:8643–8652. 2007. View Article : Google Scholar

15 

Richardson GD, Robson CN, Lang SH, et al: CD133, a novel marker for human prostatic epithelial stem cells. J Cell Sci. 117:3539–3545. 2004. View Article : Google Scholar : PubMed/NCBI

16 

Mittal S, Mifflin R and Powell DW: Cancer stem cells: the other face of Janus. Am J Med Sci. 338:107–112. 2009. View Article : Google Scholar : PubMed/NCBI

17 

Scopelliti A, Cammareri P, Catalano V, et al: Therapeutic implications of cancer initiating cells. Expert Opin Biol Ther. 9:1005–1016. 2009. View Article : Google Scholar

18 

Uchida N, Buck DW, He D, et al: Direct isolation of human central nervous system stem cells. Proc Natl Acad Sci. 97:14720–14725. 2000. View Article : Google Scholar : PubMed/NCBI

19 

Ponti D, Costa A, Zaffaroni N, et al: Isolation and in vitro propagation of tumorigenic breast cancer cells with stem/progenitor cell properties. Cancer Res. 65:5506–5511. 2005.PubMed/NCBI

20 

Bao S, Wu Q, Li Z, et al: Targeting cancer stem cells through L1CAM suppresses glioma growth. Cancer Res. 68:6043–6048. 2008. View Article : Google Scholar : PubMed/NCBI

21 

Woodward WA, Chen MS, Behbod F, et al: On mammary stem cells. J Cell Sci. 118:3585–3594. 2005. View Article : Google Scholar : PubMed/NCBI

22 

Rizzo S, Attand G and Hudson DL: Prostate epithelial stem cells. Cell Prolif. 38:363–374. 2005. View Article : Google Scholar

23 

Guo C, Liu H, Zhang BH, et al: Epcam, CD44, and CD49f distinguish sphere-forming human prostate basal cells from a subpopulation with predominant tubule initiation capability. PLoS One. 7:e342192012. View Article : Google Scholar : PubMed/NCBI

24 

Patrawala L, Calhoun T, Schneider-Broussard R, et al: Highly purified CD44+ prostate cancer cells from xenograft human tumors are enriched in tumorigenic and metastatic progenitor cells. Oncogene. 25:1696–1708. 2006.PubMed/NCBI

25 

Vander Griend DJ, Karthaus WL, Dalrymple S, et al: The role of CD133 in normal human prostate stem cells and malignant cancer-initiating cells. Cancer Res. 68:9703–9711. 2008.PubMed/NCBI

26 

Li H, Chen X, Calhoun-Davis T, Claypool K and Tang DG: PC3 human prostate carcinoma cell holoclones contain self-renewing tumor-initiating cells. Cancer Res. 68:1820–1825. 2008. View Article : Google Scholar : PubMed/NCBI

27 

Zhang K and Waxman DJ: PC3 prostate tumor-initiating cells with molecular profile FAM65Bhigh/MFI2low/LEF1low increase tumor angiogenesis. Mol Cancer. 9:3192010. View Article : Google Scholar : PubMed/NCBI

28 

Diener KR, Need EF, Buchanan G, et al: TGF-beta signalling and immunity in prostate tumourigenesis. Expert Opin Ther Targets. 14:179–192. 2010. View Article : Google Scholar : PubMed/NCBI

29 

Li X, Wang Y, Sharif-Afshar AR, et al: Urothelial transdifferentiation to prostate epithelia is mediated by paracrine TGF-beta signaling. Differentiation. 77:95–102. 2009. View Article : Google Scholar : PubMed/NCBI

30 

Tai S, Sun Y, Squires JM, et al: PC3 is a cell line characteristic of prostatic small cell carcinoma. Prostate. 71:1668–1679. 2011. View Article : Google Scholar : PubMed/NCBI

31 

Zeng Y, Kakehi Y, Nouh MA, et al: Gene expression profiles of lysophosphatidic acid-related molecules in the prostate: relevance to prostate cancer and benign hyperplasia. Prostate. 69:283–292. 2009. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Sheng X, Li Z, Wang D, Li W, Luo Z, Chen K, Cao J, Yu C and Liu W: Isolation and enrichment of PC-3 prostate cancer stem-like cells using MACS and serum-free medium. Oncol Lett 5: 787-792, 2013.
APA
Sheng, X., Li, Z., Wang, D., Li, W., Luo, Z., Chen, K. ... Liu, W. (2013). Isolation and enrichment of PC-3 prostate cancer stem-like cells using MACS and serum-free medium. Oncology Letters, 5, 787-792. https://doi.org/10.3892/ol.2012.1090
MLA
Sheng, X., Li, Z., Wang, D., Li, W., Luo, Z., Chen, K., Cao, J., Yu, C., Liu, W."Isolation and enrichment of PC-3 prostate cancer stem-like cells using MACS and serum-free medium". Oncology Letters 5.3 (2013): 787-792.
Chicago
Sheng, X., Li, Z., Wang, D., Li, W., Luo, Z., Chen, K., Cao, J., Yu, C., Liu, W."Isolation and enrichment of PC-3 prostate cancer stem-like cells using MACS and serum-free medium". Oncology Letters 5, no. 3 (2013): 787-792. https://doi.org/10.3892/ol.2012.1090
Copy and paste a formatted citation
x
Spandidos Publications style
Sheng X, Li Z, Wang D, Li W, Luo Z, Chen K, Cao J, Yu C and Liu W: Isolation and enrichment of PC-3 prostate cancer stem-like cells using MACS and serum-free medium. Oncol Lett 5: 787-792, 2013.
APA
Sheng, X., Li, Z., Wang, D., Li, W., Luo, Z., Chen, K. ... Liu, W. (2013). Isolation and enrichment of PC-3 prostate cancer stem-like cells using MACS and serum-free medium. Oncology Letters, 5, 787-792. https://doi.org/10.3892/ol.2012.1090
MLA
Sheng, X., Li, Z., Wang, D., Li, W., Luo, Z., Chen, K., Cao, J., Yu, C., Liu, W."Isolation and enrichment of PC-3 prostate cancer stem-like cells using MACS and serum-free medium". Oncology Letters 5.3 (2013): 787-792.
Chicago
Sheng, X., Li, Z., Wang, D., Li, W., Luo, Z., Chen, K., Cao, J., Yu, C., Liu, W."Isolation and enrichment of PC-3 prostate cancer stem-like cells using MACS and serum-free medium". Oncology Letters 5, no. 3 (2013): 787-792. https://doi.org/10.3892/ol.2012.1090
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team