Synovial sarcoma arising in the vulva cytogenetically confirmed by SYT break-apart rearrangement fluorescence in situ hybridization: A case report and discussion of diagnostic methods

Kawauchi,Shigeto; Ihara,Koichiro; Nishikawa,Kei; Sugino,Norihiro; Takahashi,Mutsuo; Sasaki,Kohsuke

doi:10.3892/ol.2012.857

Synovial sarcoma arising in the vulva cytogenetically confirmed by SYT break-apart rearrangement fluorescence in situ hybridization: A case report and discussion of diagnostic methods

Authors:
- Shigeto Kawauchi
- Koichiro Ihara
- Kei Nishikawa
- Norihiro Sugino
- Mutsuo Takahashi
- Kohsuke Sasaki
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Affiliations: Department of Pathology, Yamaguchi University School of Medicine, Yamaguchi 755-8505, Japan, Department of Orthopedic Surgery, Yamaguchi University School of Medicine, Yamaguchi 755-8505, Japan, Department of Gynecology, Yamaguchi University School of Medicine, Yamaguchi 755-8505, Japan, Faculty of Health Sciences, Yamaguchi University School of Medicine, Yamaguchi 755-8505, Japan
Published online on: August 8, 2012 https://doi.org/10.3892/ol.2012.857
Pages: 955-959

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Abstract

Synovial sarcoma (SS) is a soft tissue sarcoma of unknown histogenesis that rarely occurs in the female genital tract. We report a case of SS occurring in the right vulva of a young Japanese female. The tumor was composed of poorly differentiated rounded cell areas, surrounded by fibroblastic spindle-shaped cell areas. Immunohistochemically, the tumor cells were focally positive for cytokeratin, vimentin, CD99, Bcl-2 and neuron-specific enolase. The tumor was suspected, but was difficult to confirm as it was an SS based solely on light-microscopic and immunohistochemical findings. Although reverse transcription polymerase chain reaction (RT-PCR) failed to detect SS-specific SYT-SSX fusion gene transcripts using an RNA sample extracted from the formalin-fixed paraffin-embedded tumor tissue, SYT break-apart rearrangement fluorescence in situ hybridization (SYT bar-FISH) successfully confirmed our diagnosis of SS for the tumor. Thus, SYT bar-FISH may be more suitable for the purpose of the molecular diagnosis of SS than conventional RT-PCR when using archival formalin-fixed paraffin-embedded tissue specimens.

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