Synovial sarcoma arising in the vulva cytogenetically confirmed by SYT break-apart rearrangement fluorescence in situ hybridization: A case report and discussion of diagnostic methods

Kawauchi,Shigeto; Ihara,Koichiro; Nishikawa,Kei; Sugino,Norihiro; Takahashi,Mutsuo; Sasaki,Kohsuke

doi:10.3892/ol.2012.857

November 2012 Volume 4 Issue 5

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November 2012 Volume 4 Issue 5

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Case Report

Synovial sarcoma arising in the vulva cytogenetically confirmed by SYT break-apart rearrangement fluorescence in situ hybridization: A case report and discussion of diagnostic methods

Authors:
- Shigeto Kawauchi
- Koichiro Ihara
- Kei Nishikawa
- Norihiro Sugino
- Mutsuo Takahashi
- Kohsuke Sasaki
View Affiliations / Copyright

Affiliations: Department of Pathology, Yamaguchi University School of Medicine, Yamaguchi 755-8505, Japan, Department of Orthopedic Surgery, Yamaguchi University School of Medicine, Yamaguchi 755-8505, Japan, Department of Gynecology, Yamaguchi University School of Medicine, Yamaguchi 755-8505, Japan, Faculty of Health Sciences, Yamaguchi University School of Medicine, Yamaguchi 755-8505, Japan
Pages: 955-959
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Published online on: August 8, 2012

https://doi.org/10.3892/ol.2012.857
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Abstract

Synovial sarcoma (SS) is a soft tissue sarcoma of unknown histogenesis that rarely occurs in the female genital tract. We report a case of SS occurring in the right vulva of a young Japanese female. The tumor was composed of poorly differentiated rounded cell areas, surrounded by fibroblastic spindle-shaped cell areas. Immunohistochemically, the tumor cells were focally positive for cytokeratin, vimentin, CD99, Bcl-2 and neuron-specific enolase. The tumor was suspected, but was difficult to confirm as it was an SS based solely on light-microscopic and immunohistochemical findings. Although reverse transcription polymerase chain reaction (RT-PCR) failed to detect SS-specific SYT-SSX fusion gene transcripts using an RNA sample extracted from the formalin-fixed paraffin-embedded tumor tissue, SYT break-apart rearrangement fluorescence in situ hybridization (SYT bar-FISH) successfully confirmed our diagnosis of SS for the tumor. Thus, SYT bar-FISH may be more suitable for the purpose of the molecular diagnosis of SS than conventional RT-PCR when using archival formalin-fixed paraffin-embedded tissue specimens.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Synovial sarcoma arising in the vulva cytogenetically confirmed by SYT break-apart rearrangement fluorescence in situ hybridization: A case report and discussion of diagnostic methods

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