DNAJC25 is downregulated in hepatocellular carcinoma and is a novel tumor suppressor gene

Liu,Tingting; Jiang,Wei; Han,Dingding; Yu,Long

doi:10.3892/ol.2012.903

December 2012 Volume 4 Issue 6

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December 2012 Volume 4 Issue 6

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Article

DNAJC25 is downregulated in hepatocellular carcinoma and is a novel tumor suppressor gene

Authors:
- Tingting Liu
- Wei Jiang
- Dingding Han
- Long Yu
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Affiliations: State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200433, P.R. China
Pages: 1274-1280
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Published online on: September 10, 2012

https://doi.org/10.3892/ol.2012.903
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Abstract

HSP40, also known as DnaJ, is one of the subfamilies of the heat shock protein family. DnaJ/Hsp40 proteins act as co‑chaperones by binding to the chaperone Hsp70 through their J domain and stimulating ATP hydrolysis to aid protein translation, folding, unfolding, translocation and degradation. They are implicated in various human diseases, including neurodegenerative disorders and cancer. In the present study, we cloned and identified a new gene, DnaJ (HSP40) homolog, subfamily C, member 25 (DNAJC25), which is localized to the cytoplasm. Real‑time PCR revealed that the expression of DNAJC25 is particularly high in the liver and is downregulated in hepatocellular carcinoma (HCC) compared with adjacent normal tissues. The overexpression of DNAJC25 led to an inhibition of colony growth both in quantity and size. Flow cytometry analysis indicated that DNAJC25 also significantly increased cell apoptosis. Our data, therefore, indicate that DNAJC25 plays an important role in hepatocellular carcinogenesis, and should be further studied as a potential tumor suppressor candidate.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

DNAJC25 is downregulated in hepatocellular carcinoma and is a novel tumor suppressor gene

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