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Article

Involvement of the phosphoinositide 3‑kinase/Akt pathway in apoptosis induced by capsaicin in the human pancreatic cancer cell line PANC-1

  • Authors:
    • Jian-Hong Zhang
    • Fu-Ji Lai
    • Hui Chen
    • Jiang Luo
    • Ri-Yuan Zhang
    • He‑Qi  Bu
    • Zhao-Hong Wang
    • Hong-Hai Lin
    • Sheng-Zhang Lin
  • View Affiliations / Copyright

    Affiliations: Department of Hepatobiliary-Pancreatic Surgery, The Second Affiliated Hospital of Wenzhou Medical College, Wenzhou, Zhejiang 325027, P.R. China
  • Pages: 43-48
    |
    Published online on: October 24, 2012
       https://doi.org/10.3892/ol.2012.991
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Abstract

Capsaicin, one of the major pungent ingredients found in red peppers, has been recently demonstrated to induce apoptosis in various malignant cell lines through an unclear mechanism. In this study, the effect of capsaicin on proliferation and apoptosis in the human pancreatic cancer cell line PANC‑1 and its possible mechanism(s) of action were investigated. The results of a Cell Counting Kit-8 (CCK‑8) assay revealed that capsaicin significantly decreased the viability of PANC‑1 cells in a dose-dependent manner. Capsaicin induced G0/G1 phase cell cycle arrest and apoptosis in PANC‑1 cells as demonstrated by a flow cytometric assessment. Caspase‑3 expression at both the protein and mRNA level was promoted following capsaicin treatment. Furthermore, we revealed that phospho‑PI3 Kinase p85 (Tyr458) and phospho‑Akt (Ser473) in PANC‑1 cells were downregulated in response to capsaicin. Moreover, capsaicin gavage significantly inhibited the growth of pancreatic cancer PANC‑1 cell xenografts in athymic nude mice. An increased number of TUNEL‑positive cells and cleaved caspase‑3 were observed in capsaicin-treated mice. In vivo, capsaicin downregulated the expression of phospho‑PI3 Kinase p85 (Tyr458) and phospho‑Akt (Ser473). In conclusion, we have demonstrated that capsaicin is an inhibitor of growth of PANC‑1 cells, and downregulation of the phosphoinositide 3‑kinase/Akt pathway may be involved in capsaicin-induced apoptosis in vitro and in vivo.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang J, Lai F, Chen H, Luo J, Zhang R, Bu HQ, Wang Z, Lin H and Lin S: Involvement of the phosphoinositide 3‑kinase/Akt pathway in apoptosis induced by capsaicin in the human pancreatic cancer cell line PANC-1. Oncol Lett 5: 43-48, 2013.
APA
Zhang, J., Lai, F., Chen, H., Luo, J., Zhang, R., Bu, H. ... Lin, S. (2013). Involvement of the phosphoinositide 3‑kinase/Akt pathway in apoptosis induced by capsaicin in the human pancreatic cancer cell line PANC-1. Oncology Letters, 5, 43-48. https://doi.org/10.3892/ol.2012.991
MLA
Zhang, J., Lai, F., Chen, H., Luo, J., Zhang, R., Bu, H., Wang, Z., Lin, H., Lin, S."Involvement of the phosphoinositide 3‑kinase/Akt pathway in apoptosis induced by capsaicin in the human pancreatic cancer cell line PANC-1". Oncology Letters 5.1 (2013): 43-48.
Chicago
Zhang, J., Lai, F., Chen, H., Luo, J., Zhang, R., Bu, H., Wang, Z., Lin, H., Lin, S."Involvement of the phosphoinositide 3‑kinase/Akt pathway in apoptosis induced by capsaicin in the human pancreatic cancer cell line PANC-1". Oncology Letters 5, no. 1 (2013): 43-48. https://doi.org/10.3892/ol.2012.991
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang J, Lai F, Chen H, Luo J, Zhang R, Bu HQ, Wang Z, Lin H and Lin S: Involvement of the phosphoinositide 3‑kinase/Akt pathway in apoptosis induced by capsaicin in the human pancreatic cancer cell line PANC-1. Oncol Lett 5: 43-48, 2013.
APA
Zhang, J., Lai, F., Chen, H., Luo, J., Zhang, R., Bu, H. ... Lin, S. (2013). Involvement of the phosphoinositide 3‑kinase/Akt pathway in apoptosis induced by capsaicin in the human pancreatic cancer cell line PANC-1. Oncology Letters, 5, 43-48. https://doi.org/10.3892/ol.2012.991
MLA
Zhang, J., Lai, F., Chen, H., Luo, J., Zhang, R., Bu, H., Wang, Z., Lin, H., Lin, S."Involvement of the phosphoinositide 3‑kinase/Akt pathway in apoptosis induced by capsaicin in the human pancreatic cancer cell line PANC-1". Oncology Letters 5.1 (2013): 43-48.
Chicago
Zhang, J., Lai, F., Chen, H., Luo, J., Zhang, R., Bu, H., Wang, Z., Lin, H., Lin, S."Involvement of the phosphoinositide 3‑kinase/Akt pathway in apoptosis induced by capsaicin in the human pancreatic cancer cell line PANC-1". Oncology Letters 5, no. 1 (2013): 43-48. https://doi.org/10.3892/ol.2012.991
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