Involvement of the phosphoinositide 3‑kinase/Akt pathway in apoptosis induced by capsaicin in the human pancreatic cancer cell line PANC-1

Introduction

Pancreatic cancer, one of the most fatal types of solid malignancy, is the fourth leading cause of cancer-related mortality in the USA and other industrialized countries, leading to an estimated 227,000 deaths per year worldwide (1,2). Certain studies have demonstrated that the mortality rates of pancreatic cancer in China have constantly increased over the past decade. Presently, it is the eighth-leading cause of cancer-related mortality in China (3). Due to difficulties in early diagnosis and its highly aggressive malignant behavior, only 10–20% of pancreatic cancers can be surgically resected with curative intent at the time of diagnosis and the majority of patients experience local recurrence and metastasis (4). Despite therapeutic advances, the prognosis of patients with pancreatic ductal adenocarcinoma is extremely poor; the median survival time is 6 months and less than 5% survive 5 years following the initial diagnosis (1,5). Gemcitabine is the current standard therapy for advanced pancreatic cancer. However, gemcitabine treatment results in an objective tumor response rate of less than 20%, and only a marginal survival advantage is associated with multiple adverse events and drug resistance (6,7). It is therefore of particular interest to impose new therapeutic strategies and to improve the prognosis of this potentially fatal disease.

The phosphoinositide 3-kinase (PI3K) pathway is a key signal transduction system that links oncogenes and multiple receptor classes to a number of essential cellular functions, and is possibly the most commonly activated signaling pathway in human cancer (8,9). The activation of the PI3K pathway is relatively well understood; it is known to be a multi-step process involving the PI3K-dependent phosphorylation of phospholipids localized at the plasma membrane, and the subsequent membrane localization of phosphoinositide-dependent kinase 1 (PDK1) and Ser/Thr kinase Akt (also known as protein kinase B, PKB) via their pleckstrin homology (PH) domains (9,10). The activation of PI3K ultimately leads to Akt phosphorylation, and activated Akt controls fundamental cellular processes including cell survival by phosphorylating and inactivating several downstream pro-apoptotic target molecules (9,11). Another study has demonstrated that the PI3K/Akt pathway is constitutively activated in a majority of human pancreatic cancer cell lines (12). It has been revealed that inhibition of the PI3K/Akt pathway results in inhibition of tumor growth and apoptosis, and the PI3K/Akt pathway is considered as a viable and effective target for pancreatic cancer therapy (9,12,13).

Capsaicin is one of the major pungent ingredients found in red peppers, which are among the most commonly and frequently used spices in the world (14,15). Due to its analgesic activity, topical application of capsaicin has been used in clinical practice for the treatment of neuropathic pain (16,17). Capsaicin has been revealed to inhibit growth and induce apoptosis in various malignant cell lines (18–21). Moreover, capsaicin treatment has significantly suppressed the growth of tumors in athymic nude mice transplanted with cancer cells (22,23). Capsaicin was also revealed to inhibit the PI3K/Akt pathway in B16-F10 melanoma cells (24). However, data regarding the properties of capsaicin in pancreatic cancer cells remain limited and the mechanisms of apoptosis have not yet been fully elucidated. Based on these studies, we hypothesized that capsaicin may exhibit an antitumor effect and induce apoptosis in pancreatic cancer cells, via downregulation of the PI3K/Akt pathway.

In the present study, we show that capsaicin significantly inhibits the growth of PANC-1 cells and triggers apoptosis in a dose-dependent manner. Treatment of PANC-1 cells with capsaicin resulted in downregulation of phospho-PI3 Kinase p85 (Tyr458) and phospho-Akt (Ser473). Furthermore, capsaicin also inhibited the growth of pancreatic cancer PANC-1 xenograft tumors induced in athymic nude mice. These results suggest that capsaicin may be an effective and promising antitumor agent against pancreatic cancer.

Materials and methods

Reagents and antibodies

Capsaicin, dimethyl sulfoxide (DMSO), and propidium iodide (PI) were obtained from Sigma-Aldrich (St. Louis, MO, USA). RNase was obtained from Fermentas (St. Leon-Rot, Germany). Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), penicillin-streptomycin and trypsin-EDTA were obtained from Gibco BRL (Invitrogen, Grand Island, NY, USA). Rabbit cleaved caspase-3 antibody, phospho-PI3 Kinase p85 (Tyr458) antibody, phospho-Akt (Ser473) antibody and β-tubulin antibody were purchased from Cell Signaling Technology, Inc. (Beverly, MA, USA). Horseradish peroxidase (HRP)-conjugated goat anti-rabbit secondary antibody was purchased from Beyotime Biotechnology (Haimen, China).

Cell culture

The human pancreatic cancer cell line PANC-1 was purchased from Shanghai Cell Bank (Shanghai, China). The cell line was maintained in continuous exponential growth in DMEM supplemented with 10% FBS, 100 U/ml penicillin and 100 μg/ml streptomycin, at 37°C under a humidified 5% CO2 atmosphere.

Cell viability

PANC-1 cells were seeded at a density of 5×103/well in 96-well plates. Following incubation overnight, the medium was removed and replaced with fresh medium containing different concentrations of capsaicin (50, 100, 150, 200, 250 or 300 μM) or DMSO (control) for 24 h. On completion of incubation, cell viability was determined using Cell Counting Kit-8 (CCK-8; Dojindo Molecular Technologies, Kumamoto, Japan) according to the manufacturer’s instructions. CCK-8 reagent (10 μl) was added to 100 μl media in each well and incubation was continued for a further 3 h. The absorbance (A) of each well was read at 450 nm using an enzyme-linked immunosorbant assay (ELISA) reader (Bio-Tek ELx808, Winooski, VT, USA). Percentage suvival rate was calculated using the following equation: Survival rate (%) = (Asample−Ablank)/(Acontrol−Ablank).

Cell cycle analysis

PANC-1 cells were seeded at a density of approximately 5×105 cells/well into 6-well plates, cultured overnight and then 150, 200 or 250 μM capsaicin or DMSO (control) was added. Following 24 h of incubation, cells were harvested, washed with PBS and then fixed with 70% ethanol overnight at 4°C. Cells were stained with 20 μg/ml RNase and 20 μg/ml PI for 30 min at 37°C in the dark, and then analyzed by flow cytometry (Becton-Dickinson, San Jose, CA, USA).

Apoptosis assay

The measurement of phosphatidylserine redistribution in a plasma membrane was conducted according to the manufacturer’s instructions for the Annexin V-FITC/PI Apoptosis Detection kit (BioVision, Mountain View, CA, USA). Following 150, 200 or 250 μM capsaicin or DMSO (control) treatment, harvested cells were suspended in 500 μl Annexin V binding buffer. Then, 5 μl Annexin V-FITC and 10 μl PI were added and incubated with the cells for 5 min in the dark. The stained cells were analyzed directly by flow cytometry using the Cell Quest program (Becton-Dickinson).

Western blot analysis

PANC-1 cells were treated with 150, 200 or 250 μM capsaicin or DMSO (control). Following incubation, the cells were lysed in Cell Lysis buffer (20 mM Tris-HCl pH 7.5, 150 mM NaCl, 1 mM Na2 EDTA, 1 mM EGTA, 1% Triton, 2.5 mM sodium pyrophosphate, 1 mM beta-glycerophosphate, 1 mM Na3VO4, 1 μg/ml leupeptin and 1 mM PMSF; Cell Signaling Technology, Beverly, MA, USA) for 5 min on ice and then subjected to sonication for 20 sec. Protein concentrations were measured using the BCA Protein Assay kit (Pierce Biotechnology, Inc., Rockford, IL, USA). Equal amounts of protein were separated on 8 or 12% SDS-PAGE, and transferred onto a polyvinylidene difluoride membrane. Next, the membrane was blocked with 5% BSA and then incubated overnight with cleaved caspase-3 antibody, phospho-PI3 Kinase p85 (Tyr458) antibody or phospho-Akt (Ser473) antibody. Following extensive washing, the membrane was incubated with appropriate secondary antibodies conjugated with horseradish peroxidase for 1 h at room temperature. Following washing, immunoblots were developed using the Enhanced Chemiluminescence kit (Pierce).

Real-time polymerase cahin reaction (PCR)

PANC-1 cells were treated with 200 μM capsaicin or DMSO (control) for 24 h and then total RNA was isolated from treated cells using TRIzol reagent (Invitrogen). Total RNA (1 μg) was reverse transcribed in 20 μl volume, using RevertAid™ First Strand cDNA Synthesis kit (Fermentas). Reverse transcriptase reaction mixture (1 μl) was then real-time PCR-amplified in Mastercycler® ep Realplex (Eppendorf, Germany). The initial denaturation step was 95°C for 60 sec, followed by 40 cycles of amplification at 95°C for 15 sec, 60°C for 15 sec and 72°C for 45 sec. The primers used were: Caspase-3 forward 5′-CAGTGGAGGCCGACTTCTTG-3′ and reverse 5′-TGGCACAAAGCGACTGGAT-3′; RPLP0 forward 5′-GAGACAAAGTGGGAGCCAGCGA-3′ and reverse 5′-ACCCTCCAGGAAGCGAGAATGC-3′. All samples were performed in triplicate and the relative quantity of the target gene was normalized with the housekeeping gene RPLP0.

In vivo studies

BALB/c (nu/nu) four-week-old male mice were purchased from Shanghai Laboratory Animals Center (Shanghai, China) and maintained in specific pathogen-free conditions. All animal studies were approved by the Animal Research and Ethical Committee of Wenzhou Medical College (Zhejiang, China). Pancreatic cancer xenograft tumor model was performed as described in our previous studies (25,26). PANC-1 cells (5×106) in 200 μl complete culture medium were injected subcutaneously into the right flank of each mouse. Four weeks after cell inoculation, eight randomized animals for each experimental group received capsaicin (5 mg/kg body weight in 100 μl of PBS containing 0.3% ethanol) or vehicle (100 μl of PBS containing 0.3% ethanol) by gavage three days a week (Monday, Wednesday and Friday) for four weeks. One week after the last treatment, the mice were sacrificed. The tumors were weighed with an electronic balance and tumor volumes were calculated with a vernier caliper using the following formula: (4π/3) × (width/2)2 × (length/2). Half of the tumor tissue in each group was formalin-fixed and paraffin-embedded for TUNEL assay. Remaining tumor tissue was stored in liquid nitrogen for western blot analysis. Western blot analysis in tumor tissue was performed as previously described in vitro.

In situ detection of apoptotic cells in tumor tissues

Apoptotic cells in the tumor tissues were detected by TUNEL assay, according to the manufacturer’s instructions for the In Situ Cell Death Detection kit (Roche, Mannheim, Germany). Sections were deparaffinized in xylene and then treated with a graded series of alcohol (100, 95, 90, 80 and 70% ethanol in double-distilled water) and rehydrated in PBS (pH 7.5). Tissues were then treated with proteinase K solution for permeabilization and treated with TUNEL reaction mixture, then incubated at 37°C for 1 h. Apoptotic cells were photographed under a fluorescence microscope (Nikon, Tokyo, Japan).

Statistical analysis

Data are represented as mean ± standard deviation for the absolute values or percentage of controls. SPSS13.0 software (SPSS, Inc., Chicago, IL, USA) was used for statistical analysis. Differences between the capsaicin-treated and DMSO-treated (control) groups were analyzed by an unpaired Student’s t-test or ANOVA. P<0.05 was considered to indicate a statistically significant difference.

Results

Effect of capsaicin on cell proliferation

To investigate the effect of capsaicin on cell growth, PANC-1 cells were treated with increasing concentrations of capsaicin (0–300 μM) for 24 h. Cell viability was determined by CCK-8 assay. As demonstrated in Fig. 1, cell growth was inhibited by capsaicin treatment in a dose-dependent manner with an IC50 of ∼200 μM.

Figure 1 Evaluation of cell viability by Cell Counting Kit-8 (CCK-8) assay as described in Materials and methods. PANC-1 cells were treated with different concentrations of capsaicin for 24 h. On termination of incubation, viable cells were evaluated relative to dimethyl sulfoxide (DMSO)-treated cells (control) and interpreted as percentage of viable cells. Data obtained from three separate experiments are expressed as mean ± standard deviation and *P<0.01, compared with the control group.

Capsaicin induces G0/G1 phase arrest in PANC-1 cells

We investigated whether the antiproliferative activity of capsaicin in PANC-1 cells was correlated with cell cycle arrest. As demonstrated in Fig. 2, following capsaicin treatment, cell cycle analysis revealed that capsaicin increased the number of cells in the G0/G1 phase in a dose-dependent manner.

Figure 2 Capsaicin induces G0/G1 phase arrest. Treated cells were stained with propidium iodide (PI) and DNA content was analyzed by flow cytometry. The number of cells in each phase was calculated using ModFit software. The results shown are representative of three independent experiments.

Capsaicin triggers apoptosis in PANC-1 cells

As demonstrated in Fig. 3A, treatment with capsaicin induced a greater level of apoptosis in PANC-1 cells, as revealed by flow cytometric assessment. Apoptotic rates in 250 μM capsaicin-treated cells and control cells were 19.95±0.76% and 10.21±0.45%, respectively. As demonstrated in Fig. 3B, a dose-dependent increase in the cleaved caspase-3 was observed after the exposure of cells to increasing concentrations of capsaicin. Compared with DMSO-treated cells, the level of caspase-3 mRNA expression was higher (1.44-fold) following 200 μM capsaicin treatment (Fig. 3C).

Figure 3 Capsaicin triggers apoptosis in PANC-1 cells. (A) Apoptotic cells were assessed by qualitative flow cytometry using Annexin V and propidium iodide (PI) staining. Cells in the lower left quadrant (Annexin V-FITC−/PI−) are viable, those in the lower right quadrant (Annexin V-FITC+/PI−) are early apoptotic and those in the upper right quadrants (Annexin V-FITC+/PI+) are late apoptotic or necrotic. Data obtained from three separate experiments are expressed as mean ± standard deviation (SD) and *P<0.01, compared with the control group. (B) Cleaved caspase-3 examined by western blot analysis. β-tubulin was used as a loading control. Results demonstrated are representative of three independent experiments. (C) mRNA expression of caspase-3 examined by real-time PCR. The relative quantity of the target gene was normalized with the housekeeping gene RPLP0. Data are expressed as mean ± SD and *P<0.01, compared with control group.

Downregulation of PI3K/Akt pathway by capsaicin in PANC-1 cells

To elucidate the mechanism of antiproliferation and apoptosis of capsaicin in PANC-1 cells, we employed western blot analysis for phospho-PI3 Kinase p85 (Tyr458) and phospho-Akt (Ser473). As demonstrated in Fig. 4, capsaicin significantly downregulated the expression of phospho-PI3 Kinase p85 (Tyr458) and phospho-Akt (Ser473) in a dose-dependent manner.

Figure 4 Effect of capsaicin on protein expression of phospho-PI3 Kinase p85 (Tyr458) and phospho-Akt (Ser473) in PANC-1 cells. Western blot analysis was performed as described in Materials and methods. β-tubulin was used as a loading control. Results demonstrated are representative of three independent experiments.

Antitumoral effect of capsaicin in vivo

To investigate the antitumoral effect of capsaicin on pancreatic cancer cells in vivo, we first generated pancreatic cancer xenograft tumors in athymic nude mice. One week after the last treatment, the mice were sacrificed and the tumors were weighed. Tumor volumes were also measured. As demonstrated in Fig. 5A, the weights of tumors in vehicle-treated mice were ∼1.43-fold greater than that of capsaicin-treated mice. Tumor volumes in capsaicin-treated mice and vehicle-treated mice were 617.25±85.07 mm3 and 921.16±110.29 mm3, respectively (Fig. 5B). As demonstrated in Fig. 5C, increased TUNEL-positive cells were observed in capsaicin-treated mice. Consistent with our studies in vitro, cleaved caspase-3 was observed in capsaicin-treated mice and capsaicin downregulated the expression of phospho-PI3 Kinase p85 (Tyr458) and phospho-Akt (Ser473) (Fig. 5D).

Figure 5 Effect of capsaicin on a pancreatic cancer xenograft in nude mice. (A) Tumor weights. Data are expressed as mean ± standard deviation (SD) and *P<0.05, compared with vehicle-treated mice. (B) Tumor volumes. Data are expressed as mean ± SD and *P<0.05, compared with vehicle-treated mice. (C) Apoptotic cells evaluated by TUNEL assay in tumor tissues. (D) Western blot analysis for cleaved caspase-3, phospho-PI3 Kinase p85 (Tyr458) and phospho-Akt (Ser473). β-tubulin was used as a loading control. The results shown are representative of three independent experiments.

Discussion

Epidemiologic studies have revealed that several dietary agents modulate diverse biochemical processes involved in carcinogenesis (27). These include inhibition of carcinogen activation, cellular proliferation and tumor metastasis, blockading tumor cell cycle progression and induction of apoptosis (27). In vitro and in vivo studies have shown that dietary chemopreventive agents may serve as potent agents for enhancing the therapeutic effects of chemotherapy, radiotherapy or other standard therapeutics for the treatment of human cancers (28). Capsaicin is the principal pungent ingredient present in red peppers, which are among the most frequently consumed spices worldwide (14,15). Capsaicin has been revealed to possess inhibitory effects in various cancer cells (18–20,22,24). However, the precise molecular mechanisms have not been well elucidated in pancreatic cancer cells. In this study, we first used a CCK-8 assay to detect cell viability following capsaicin treatment. The results revealed that capsaicin significantly inhibited PANC-1 cell proliferation in a dose-dependent manner. Furthermore, capsaicin gavage significantly inhibited the growth of pancreatic cancer PANC-1 cell xenografts in athymic nude mice.

Next, we investigated whether the antiproliferative activity of capsaicin in PANC-1 cells was due to cell cycle arrest and apoptosis. In the present study, capsaicin treatment in PANC-1 cells induced G0/G1 phase arrest and apoptosis in a dose-dependent manner. The results of the TUNEL assay revealed that increased numbers of apoptotic cells were observed in capsaicin-treated mice. Previously, accumulated evidence indicated that caspases, a family of cysteine proteases, play a pivotal role in the apoptotic process; caspase-3 is an apoptosis executioner and is activated by other activated caspases, including caspase-8 and 9 (29,30). Activated caspase-3 subsequently cleaves certain specific substrates, including poly (ADP-ribosyl) polymerase (PARP) and D4-GDI proteins, which are important for the occurrence of typical biochemical and morphological changes in apoptotic cells (29,31). To further confirm that the antiproliferative activity of capsaicin was due to apoptosis, we examined caspase-3 activation, an event that is commonly used as a hallmark of apoptosis. In this study, caspase-3 was activated after capsaicin treatment in vitro and in vivo. These results suggest that apoptosis may be a potential general mechanism and provide a mechanistic basis for the antiproliferative as well as anti-neoplastic effects of capsaicin in PANC-1 cells.

To further elucidate the mechanism of antiproliferation and apoptosis of capsaicin in PANC-1 cells, we investigated the PI3K/Akt pathway. It has been demonstrated that the PI3K/Akt signaling pathway components are frequently altered in human cancers and inappropriately activated (9,11). PI3Ks are divided into three classes according to their structural characteristics and substrate specificity. Of these, the most commonly studied are the class I enzymes that are activated directly by cell surface receptors. Class I PI3Ks are further divided into class IA enzymes, which are activated by RTKs, GPCRs and certain oncogenes including the small G protein RAS, and class IB enzymes, which are regulated exclusively by GPCRs. Class IA PI3Ks are heterodimers consisting of a p110 catalytic subunit and a p85 regulatory subunit (8,9). In the present study, the phosphorylation level of PI3K at Tyr458 of the p85 regulatory subunit was significantly reduced in capsaicin-treated cells as compared with the control experiment. Akt, a serinethreonine kinase that is directly activated in response to PI3Ks, is a major effector of PI3Ks in cancer (9). In this study, the phosphorylation level of Akt at Ser473 (one of the two target amino acids whose phosphorylation upregulates Akt kinase activity) was significantly downregulated in response to capsaicin treatment. Furthermore, downregulated expression of phospho-PI3 Kinase p85 (Tyr458) and phospho-Akt (Ser473) were observed in capsaicin-treated mice. These results suggest that downregulation of the PI3K/Akt signaling pathway may be involved in capsaicin-induced apoptosis in PANC-1 cells.

Activated Akt phosphorylates several cellular proteins, including glycogen synthase kinase-3α (GSK-3α), GSK-3β, forkhead box O transcription factors (FoxO), murine double minute 2 (MDM2), B-cell lymphoma-2 (BCL2)-interacting mediator of cell death (BIM) and BCL2-associated agonist of cell death (BAD), to facilitate cell survival and cell cycle entry (8,9). However, it remains unknown how activation of the PI3K/Akt signaling pathway promotes cell survival and suppresses apoptosis. It has been demonstrated that inhibition of GSK3β, one of the effectors downstream of Akt, leads to G0/G1 phase arrest (32,33). In this study, capsaicin treatment induced G0/G1 phase arrest and downregulation of the PI3K/Akt pathway in PANC-1 cells. Therefore, downregulation of the PI3K/Akt/GSK3β pathway may be involved in capsaicin-induced G0/G1 phase arrest and apoptosis, which indeed needs further investigation.

Together, our studies suggest that capsaicin-induced apoptosis may correlate with downregulation of the PI3K/Akt pathway. Thus, the present study provides novel insights into the molecular mechanisms of capsaicin in panreatic cancer cells. These findings strengthen the idea that capsaicin may be used as an anti-neoplastic medicine and that the PI3K/Akt pathway is a promising target for therapeutic intervention in pancreatic cancer.

Acknowledgements

The authors are grateful for funding support from the Administration of Traditional Chinese Medicine of Zhengjing Province, China (Grant No. 2011ZZ010), Zhejiang Provincial Science Fund for Distinguished Young Scholars (Grant No. LR12H280001) and The National Natural Science Foundation of China (Grant No. 81173606).

References