DNA hypomethylation of interleukin 8 in clear cell renal cell carcinoma

Yoo,Koo  Han; Park,Yong-Koo; Chang,Sung-Goo

doi:10.3892/ol.2012.992

January 2013 Volume 5 Issue 1

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January 2013 Volume 5 Issue 1

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Article

DNA hypomethylation of interleukin 8 in clear cell renal cell carcinoma

Authors:
- Koo Han Yoo
- Yong-Koo Park
- Sung-Goo Chang
View Affiliations / Copyright

Affiliations: Department of Urology, School of Medicine, Kyung Hee University, Seoul 130-702, Republic of Korea, Department of Pathology, School of Medicine, Kyung Hee University, Seoul 130-702, Republic of Korea
Pages: 39-42
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Published online on: October 24, 2012

https://doi.org/10.3892/ol.2012.992
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Abstract

DNA hypomethylation is associated with carcinogenesis due to its involvement in cancer initiation and progression. In this study we analyzed the hypomethylation status of candidate genes in clear cell renal cell carcinoma (CCRCC) using a large-scale, high-throughput DNA methylation profiling technique. Results revealed that hypomethylated interleukin 8 (IL8) gene was the most prominent of the candidate genes, with a beta value difference of 0.406 (cancer tissue mean beta value, 0.346; normal tissue mean beta value, 0.752). Validation results using sequencing analysis demonstrated that the methylation rate was 2.4% in cancer tissue and 14.7% in normal tissue, whilst the non-methylation rate was 82.9% in cancer tissue and 52.9% in normal tissue. However, the hypomethylation status of IL8 had no significant relationship with Fuhrman's nuclear grade, tumor node metastasis (TNM) stage or survival (P>0.05). We revealed that the IL8 gene is maximally hypomethylated in cancer tissue compared to normal tissue.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

DNA hypomethylation of interleukin 8 in clear cell renal cell carcinoma

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